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journal1 ›› 2018, Vol. 34 ›› Issue (4): 527-532.DOI: 10.16409/j.cnki.2095-039x.2018.04.005

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Cloning, Sequence and Expression Analysis of Cuticle Protein Gene of Helicoverpa armigera

WANG Xiaobing, DENG Denghui, ZHANG Kun, GUO Jianglong, HAN Weili, WU Shaoying   

  1. College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China
  • Received:2018-03-27 Online:2018-08-08 Published:2018-07-30

Abstract: Insect cuticle protein can help insects resist adverse environmental conditions and play an important role in the growth and development of insects. The cDNA sequence of the cuticle protein gene was cloned based on the information of the transcriptome of Helicoverpa armigera (Hübner) and the relative expression levels of the cuticle protein at different ages and tissues were investigated by Real-time PCR. A 612 bp HAPFL67 cDNA open reading frame (KP072002) was obtained in the bollworm, named HACPFL67, encoding 204 amino acids with a molecular weight of approximately 20.892 kDa and an isoelectric point of 7.275. The sequence contains a conserved sequence of cuticle protein, and is linked to Lepidoptera. Noctuidae Heliothis virescens and Spodoptera litura are closely related, among which the amino acid sequence similarity of the tobacco budworm is above 83%. The expression of HACPFL67 in cotton bollworms at different developmental stages and tissues was very different, and the fourth-instar larvae had the highest expression at various developmental stages. In summary, the relative expression levels of HACPFL67 in cotton bollworms indicate differences in different tissues and developmental stages.

Key words: Helicoverpa armigera, insect cuticle protein, cloning, quantitative Real-time

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