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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (5): 1109-1116.DOI: 10.16409/j.cnki.2095-039x.2022.09.007


Development of an Event-Specific Droplet Digital PCR Assay for Quantification of the Transgene DNAs in Trace Samples of GM Goat hLZ-J1

WANG Yijie1, XU Wenting1, YANG Litao1, LIANG Jingang2   

  1. 1. School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;
    2. Development Center of Science and Technology, Ministry of Agriculture of People's Republic of China, Beijing 100176, China
  • Received:2021-07-15 Published:2022-10-20

Abstract: Human lysozyme (hLZ) is a natural antibiotic,functioning to resist bacteria,fungi,inflammation and infection.Transgenic human lysozyme goat can express high levels of human lysozyme in milk for large scale production of lysozyme.Herein,we developed an event-specific droplet digital PCR (ddPCR) method for quantitative detection of transgenic hLZ goat and its derivates.This method presents high specificity,sensitivity and accuracy in goat blood and goat milk samples analysis.The limit of detection and limit of quantification were determined with 15 copies per reaction.Furthermore,this method was used to trace the transgenic hLZ goat content in goat feces and the living environmental soil samples,indicating that the developed ddPCR method can be used for monitoring horizontal gene transfer and environmental risk assessment of transgenic hLZ goat.

Key words: ddPCR, genetically modified human lysozyme goat, event-specific, trace samples

CLC Number: