Screening of Trichoderma and Their Efficiency Against Sunflower Sclerotinia Rot

doi:10.16409/j.cnki.2095-039x.2022.04.001

Abstract

Abstract: Three Trichoderma strains,T.atroviride N862,T.longibrachiatum N246 and T.harzianum X102,were screened for control of sunflower sclerotinia rot by plate confrontation,detached leaf detection,pot and field trials.The growth inhibition rates of the three Trichoderma strains against S.sclerotiorum,pathogen of sunflower sclerotinia rot,were all over 85%.The inhibition rates of N862,N246 and X102 on the expansion of leaf lesions on detached leaves were 90.6%,96.5% and 63.8%,respectively.Soil amendment followed by plant drenching with Trichoderma spores gave the best control effect against sunflower sclerotinia rot,with the efficacy of over 73% for all the three strains in pot trials,and 67.54%,49.96% and 49.23% by N862,N246 and X102 in field experiments,respectively.N863 showed the highest control effect and all three Trichoderma strains are promising for practical application.

Key words: Trichoderma, sunflower, Sclerotinia sclerotiorum, control efficacy

CLC Number:

S476

CHEN Yurong, CAO Qiulin, LIAN Hua, MA Guangshu, JIANG Xiliang, LI Mei. Screening of Trichoderma and Their Efficiency Against Sunflower Sclerotinia Rot[J]. Chinese Journal of Biological Control, 2022, 38(4): 846-851.

References

[1] 郑晓薇.日葵菌核病致病菌的拮抗菌株筛选与鉴定[D].北京:北京理工大学, 2017.
[2] 王景华,王红梅.向日葵菌核病防治技术[J].农业技术与装备, 2008(11):43.
[3] 赵伟权.向日葵菌核病制病机理和综合防治技术[J].农业与技术, 2017, 37(16):58-59.
[4] 张海洋,李海燕,孟庆林,等.啶酰菌胺对向日葵核盘菌生物活性的影响[J].农学学报, 2021, 11(1):71-74.
[5] 朱林,张莹,郑晓薇,等.向日葵菌核病拮抗菌的筛选、鉴定及防效测定[J].微生物学通报, 2021, 48(12):4688-4695.
[6] 张一名,李小娟,王颖,等.抑制向日葵核盘菌菌核形成的生防菌S-16的鉴定及其生防作用研究[J].中国生物防治学报, 2014, 30(1):121-127.
[7] 王祺,张一名,赵君,等.枯草芽孢杆菌S-16抑菌活性物质的理化性质及培养条件的研究[J].中国生物防治学报, 2015, 31(3):439-444.
[8] 李小娟.向日葵菌核病生防菌的筛选鉴定及其综合防治[D].呼和浩特:内蒙古农业大学, 2013
[9] 侯启会,杜秉海,靳奉理,等.向日葵菌核病拮抗菌XRK5的筛选与鉴定[J].微生物学通报, 2010, 37(8):101-105.
[10] 王春,王芊,张匀华.木霉菌株M1M2对向日葵菌核病防治效果研究[J].宁夏农林科技, 2015, 56(7):51-52.
[11] 邵红涛,许艳丽,李春杰,等.筛选用于防治大豆尖孢镰刀菌根腐病的木霉菌株[J].中国油料作物学报, 2004, 26(4):74-77.
[12] 马光恕,梁枭,李梅,等.木霉菌对黄瓜生理特性及立枯病防治效果的影响[J].中国生物防治学报, 2021, 37(2):277-285.
[13] 霍秀娟,李朝生,韩美丽,等.灰梨孢菌产孢培养基筛选[J].南方农业学报, 2012, 43(5):605-608.
[14] 刘增亮,李梅,张玉杰,等.哈茨木霉Thga1基因敲除突变株对几种植物病原真菌的离体拮抗作用[J].中国生物防治学报, 2014, 30(3):470-475.
[15] 裴丹,杭丽雪,于景苑,等.向日葵菌核病生物防治的初步研究[J].北方农业学报, 2018, 46(6):87-92.
[16] 陈娴,李咏梅,曾艳,等.油菜菌核病拮抗内生菌的筛选及其生防效果研究[J].湖南农业科学, 2021(8):63-66.
[17] 宗兆锋,康振生.植物病理学原理(2版)[M].北京:中国农业出版社, 2010:247.
[18] Harman G E, Howell C R, Viterbo A, et al. Trichoderma species:Opportunistic, avirulent plant symbionts[J]. Nature Reviews Microbiology, 2004, 2(1):43-56.
[19] Li M, Ma G, Lian H,et al. The effects of Trichoderma on preventing cucumber fusarium wilt and regulating cucumber physiology[J]. Journal of Integrative Agriculture, 2019, 18(3):607-617.
[20] Zhao J, Liu T, Liu W C, et al. Transcriptomic insights into growth promotion effect of Trichoderma afroharzianum TM2-4 microbial agent on tomato plants[J]. Journal of Integrative Agriculture, 2021, 20(5):1266-1276.
[21] Yani S, Opik T, Belinda N, et al. Role of biological agents Trichoderma sp. at growth three garlic varieties (Allium sativum)[J]. Journal of Physics:Conference Series, 2019, 1402(3):033043.
[22] Elkelish A A, Alhaithloul H A S, Qari S H, et al. Pretreatment with Trichoderma harzianum alleviates waterlogging-induced growth alterations in tomato seedlings by modulating physiological, biochemical, and molecular mechanisms[J]. Environmental and Experimental Botany, 2020, 171:103946.
[23] 孙冬梅,杨谦,宋金柱.绿木霉菌代谢产物对大豆菌核病核盘菌的抑菌能力研究[J].天然产物研究与开发, 2005(6):691-695.
[24] 郑晓薇.向日葵菌核病致病菌的拮抗菌株筛选与鉴定[D].北京:北京理工大学, 2017.