Application of Digital PCR in the Detection of Genetically Modified Plants

doi:10.16409/j.cnki.2095-039x.2022.09.012

Abstract

Abstract: In response to the growing plantation of genetically modified plants,new demands for detecting transgenic components have arisen.Recent years have witnessed a rapid increase in the use of digital PCR to detect transgenic components as a new molecular biology technology.This paper introduces the concept of digital PCR as well as its advantages in the detection of transgenic components.It also summarizes the application of digital PCR in quantitative analysis of transgenic components,preparation of reference materials,and determination of foreign gene copy numbers in genetically modified plants.

Key words: genetically modified plants, digital PCR, detection method

CLC Number:

S188

YOU Yinghua, ZHAO Zhi, LI Yanan, SONG Zhiguo, CHANG Honglei. Application of Digital PCR in the Detection of Genetically Modified Plants[J]. Chinese Journal of Biological Control, 2022, 38(5): 1143-1148.

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