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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (5): 1143-1148.DOI: 10.16409/j.cnki.2095-039x.2022.09.012


Application of Digital PCR in the Detection of Genetically Modified Plants

YOU Yinghua1, ZHAO Zhi1, LI Yanan2, SONG Zhiguo2, CHANG Honglei3   

  1. 1. Pro-life (Henan) Biotechnology Co., Ltd, Zhengzhou 450008, China;
    2. Pro-life (Hebei) Biotechnology Co., Ltd, Shijiazhuang 050221, China;
    3. Pro-life Biotechnology Co., Ltd, Beijing 100081, China
  • Received:2021-07-15 Published:2022-10-20

Abstract: In response to the growing plantation of genetically modified plants,new demands for detecting transgenic components have arisen.Recent years have witnessed a rapid increase in the use of digital PCR to detect transgenic components as a new molecular biology technology.This paper introduces the concept of digital PCR as well as its advantages in the detection of transgenic components.It also summarizes the application of digital PCR in quantitative analysis of transgenic components,preparation of reference materials,and determination of foreign gene copy numbers in genetically modified plants.

Key words: genetically modified plants, digital PCR, detection method

CLC Number: