Isolation, Identification and Evaluation of Biocontrol Bacteria against Cucumber Target Spot

doi:10.16409/j.cnki.2095-039x.2023.02.002

Abstract

Abstract: Cucumber target spot caused by Corynespora cassiicola is a serious disease of cucumber in recent years. Bacterial strain HMB28521 with potential biocontrol ability for cucumber target spot was obtained by dual culture method and pot experiment in greenhouse. Strain HMB28521 was identified as Bacillus velezensis based on morphological, physiological, biochemical characteristics, 16S rDNA sequence analysis, as well as phylogenetic tree inferred from the alignment sequence of gyrA, gyrB, rpoB and rpoC. Lipopeptides iturin, fengycin, and surfactin were isolated, purified and identified from strain HMB28521 by fast protein liquid chromatography (FPLC) and UPLC-Triple TOF-MS/MS. The iturin and fengycin showed strong inhibitory abilities against the growth of C. cassiicola, as well as causing abnormal hyphal growth in vitro. This study provided new resources for the biological control of cucumber target leaf spot and a theoretical basis for further clarifying the action mechanism of the biocontrol bacteria.

Key words: Corynespora cassiicola, biocontrol bacteria, antifungal activity, lipopeptide

CLC Number:

S476

ZHANG Xiaoyun, Cong Rong, GUO Qinggang, LU Xiuyun, SU Zhenhe, CHEN Xiuye, LI Shezeng, MA Ping. Isolation, Identification and Evaluation of Biocontrol Bacteria against Cucumber Target Spot[J]. Chinese Journal of Biological Control, 2023, 39(1): 194-203.

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