Classification and Identification of Streptomyces aureoverticillatus DF06 and Its Effect on Disease Control and Growth Promotion

doi:10.16409/j.cnki.2095-039x.2023.02.011

Abstract

Abstract: This study aimed to investigate control efficiency and biocontrol mechanism of strain DF06 against Fusarium oxysporum f. sp. cucumerinum. DF06 was classified and identified by culture characteristics, physiological and biochemical characteristics and 16S rDNA sequence analysis. The agar plate method was used to determine the antibacterial activity of the strain on the growth of F. oxysporium f. sp. cucumerinum FOC mycelium. The control efficiency of strain DF06 to cucumber disease and its effect on the growth potential and the changes of defense enzymes in cucumber plants were determined by using pot experiments. Real-time quantitative technique was used to determine the resistance-related genes. The results showed that the strain DF06 was identified as Streptomyces aureoverticillatus, which had a strong inhibitory effect on F. oxysporium f. sp. cucumerinum FOC, with the inhibition rate of 80.80% and malformed hyphae. The control efficiency of strain DF06 to cucumber Fusarium wilt was 68.42%, higher than that of the control agent. Different concentrations of DF06 fermentation broth can promote the growth of cucumber seeds and seedlings. The plant height, chlorophyll content and dry weight increased significantly after treatment with 10⁷ CFU/mL of DF06 fermentation broth, reaching to 23.56 cm, 2.16 mg/g and 0.84 g, respectively. At the same time, the bacteria induced the up-regulation of the expression of disease resistance-related genes and improved the activities of related defense enzymes in cucumber plants. The activities of CAT, PAL and POD all increased rapidly within 24 h after treatment. DF06 strain can effectively control F. oxysporium f. sp. cucumerinum FOC by directly inhibiting the mycelium growth and inducing the plant resistance to disease. DF06 strain has the potential to be developed into biocontrol agent for the control of cucumber Fusarium wilt.

Key words: cucumber Fusarium wilt, Streptomyces aureoverticillatus, growth promoting, biocontrol mechanism

CLC Number:

S476

SHI Yifei, GENG Peibing, WU Hao, MIAO Jianqiang, HAN Lirong, LIU Xili. Classification and Identification of Streptomyces aureoverticillatus DF06 and Its Effect on Disease Control and Growth Promotion[J]. Chinese Journal of Biological Control, 2023, 39(2): 407-417.

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