Identification and Mechanism of Strain YB-1503 for Biological Control of Meloidogyne incognita

doi:10.16409/j.cnki.2095-039x.2023.02.017

Abstract

Abstract: Root-knot nematode Meloidogyne incognita is a global plant parasitic nematode that can cause poor plant growth and even death in severe cases. In order to excavate biocontrol strains against root-knot nematode with high efficiency, 24 strains of bacteria were isolated from the tomato root infected with root-knot nematode by plate dilution method. To screen high efficiency biocontrol strains, the lethal activity of cultures to second-stage juveniles (J2s) and their control effect in the greenhouse were determined. The results showed that strain YB-1503 had the highest adjusted mortality of J2s, reaching 70.0% at 48 h. Greenhouse experiment demonstrated that treatments with cultures of strain YB-1503 reduced root-knot index by 65.8%, along with increased plant growth. Strain YB-1503 was identified as Bacillus firmus by morphological，physiological and molecular biological methods. In addition, fermentation filtrates of strain YB-1503 could significantly reduce the activities of catalase, carboxylesterase and acetylcholinesterase of J2s. The content of reactive oxygen species (ROS) of J2s was also increased after being treated by fermentation filtrate of strain YB-1503, resulting in the death of nematode. Thus, YB-1503 strain has great potential for further development and utilization.

Key words: Meloidogyne incognita, Bacillus firmus, biological control, biocontrol mechanism

CLC Number:

S476

FU Yanyan, ZHANG Jie, ZHU Wenqian, XIA Mingcong, SUN Runhong, XU Wen, LANG Jianfeng, PAN Yamei, WU Chao, YANG Lirong, WU Yanbing, LI Dongmei. Identification and Mechanism of Strain YB-1503 for Biological Control of Meloidogyne incognita[J]. Chinese Journal of Biological Control, 2023, 39(2): 429-437.

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