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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (3): 673-680.DOI: 10.16409/j.cnki.2095-039x.2022.02.019

• RESEARCH REPORTS • Previous Articles    

Identification of a Biocontrol Bacterium Strain against Fusarium graminearum and Its Preliminary Study on Biocontrol Mechanism

ZHANG Zhen, QIU Haiping, CHAI Rongyao, HAO Zhongna, WANG Jiaoyu, WANG Yanli, SUN Guochang   

  1. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences/State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products/National Experiment Station for Crop Disease and Pest Resistance Evaluation, Hangzhou 310021, China
  • Received:2021-09-28 Published:2022-06-20

Abstract: In this study, a bacterial strain hzq1601 antagonizing against Fusarium graminearum was isolated from rice stubble by plate antagonism test, and was identified as Bacillus amyloliquefaciens according to its 16S rRNA and gyrB gene sequences. Compatibility analysis between this strain and carbendazim fungicide showed that the strain hzq1601 survived normally in 1000 mg/L carbendazim solution. The results of field experiment showed that the control effect of 108 cfu/mL hzq1601 fermentation broth respectively combined with 50% carbendazim WP at the dose of 80 g/667 m2 and 70 g/667 m2 on fusarium head blight were 87.1% and 81.0%, that was comparable to that of 50% carbendazim WP at the dose of 100 g/667 m2 alone, and significantly higher than those of 50% carbendazim WP at the dose of 80 g/667 m2 or 70 g/667 m2 alone. The results showed that the combined use of fermentation broth and carbendazim could reduce the application amount of carbendazim, improve the control effect on fusarium head blight. Genomic analysis suggested that the strain hzq1601 harbored 13 secondary metabolite biosynthetic gene clusters, which implied that B.amyloliquefaciens hzq1601 exhibited a multifaceted biocontrol potential.

Key words: Bacillus amyloliquefaciens, wheat fusarium head blight, combined application of carbendazim, biocontrol mechanism

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