Isolation & Identification of the Die Back Pathogen from Dendrobium officinale and Control Effect by Streptomyces diastatochramogenes Metabolites

doi:10.16409/j.cnki.2095-039x.2023.02.045

Abstract

Abstract: Die back is a common disease of Dendrobium officinale in Shouxianggu planting base, Wuyi County, Zhejiang Province. This disease seriously affects the yield and quality of D. officinale. In this study, the pathogen of D. officinale die back was isolated and confirmed by culturing the die back tissue, isolation and purification of the pathogen and verification via Koch's rule. Furthermore, the pathogen was identified as Alternaria alternata by morphological observation and gene sequence analysis (Alt a1,EF-1α, RPB2, ATP, His 3). In order to inhibit the pathogen, the fermentation products of Streptomyces diastatochromogenes 1628 was applied and showed strong inhibit effect on hyphal growth of A. alternata, and IC₅₀ was 0.461% (volume concentration). Field experiment results showed that the control effects on die back disease were 68.35% and 65.97% at 7 days and 14 days, respectively, which were higher than that of 50% carbendazim (positive control). This study showed the taxonomic status of the pathogen of D. officinale die back, and tested the biocontrol effect of S. diastochromogenes 1628 fermentation products. Our research results provide a basis for the prevention and control of D. officinale die back.

Key words: Dendrobium officinale, die back, identification, Alternaria alternata, Streptomyces diastochromogenes 1628

CLC Number:

S476

LEI Chengze, ZHANG Nuo, SONG Yang, YU Xiaoping, SHENTU Xuping. Isolation & Identification of the Die Back Pathogen from Dendrobium officinale and Control Effect by Streptomyces diastatochramogenes Metabolites[J]. Chinese Journal of Biological Control, 2023, 39(4): 942-949.

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