Identification and Control Effect of Antagonistic Streptomyces SM 3-7 against Maize Stalk Rot Disease

doi:10.16409/j.cnki.2095-039x.2022.08.004

Abstract

Abstract: In order to obtain an antagonistic bacterium with good control effect against maize stalk rot disease, the antagonistic bacteria were isolated from the rhizosphere soil of maize, with Fusarium verticillioides as the target fungi. The antibacterial activity of different strains was determined by the confrontation method, and the strains with the best antibacterial effect were selected by inhibition zone method. The selected strains were identified and its bacteriostatic spectrum and control effect were determined. The results showed that the strain SM3-7 had obvious inhibitory effect on F. verticillioides. Based on morphological, physiological, biochemical characteristics and 16S rDNA phylogenetic analysis, the strain SM3-7 was identified as Streptomyces sclerogranulatus. This strain had a broad antibacterial spectrum and had good inhibitory effect against 8 plant pathogens on corn, and inhibition zone diameter was (28.03±0.45) mm against F. verticillioides. In pot experiment, the control efficacies of strain SM3-7 against maize stalk rot on ‘Liangyu 99’ and ‘Quanyu 1233’ varieties reached 77.28% and 76.12% in 14 days, 77.28% and 76.12% in 21 days, respectively. The results showed that SM3-7 fermentation broth could effectively control maize stalk rot, which could lay a theoretical foundation for the development of biological control products against maize stalk rot.

Key words: maize stalk rot disease, Streptomyces sclerogranulatus, screening, identification, biological control

CLC Number:

S476

HOU Xiangyu, WU Hanxiang, ZHANG Jie, HUANG Peng, YAO Jinai, YU Deyi. Identification and Control Effect of Antagonistic Streptomyces SM 3-7 against Maize Stalk Rot Disease[J]. Chinese Journal of Biological Control, 2022, 38(6): 1526-1533.

References

[1] 樊伟民. 玉米茎腐病的研究现状及防治策略[J]. 黑龙江农业科学, 2022(3): 76-80.
[2] 石明亮, 黄小兰, 陆虎华, 等. 玉米抗茎腐病研究进展及其鉴定与育种方法探讨[J]. 江苏农业科学, 2017, 45(4): 1-4.
[3] 吴之涛, 杨克泽, 马金慧, 等. 玉米茎基腐病研究进展[J]. 安徽农业科学, 2018, 46(22): 5-7..
[4] 刘春来. 中国玉米茎腐病研究进展[J]. 中国农学通报, 2017, 33(30): 130-134.
[5] 沈广爽, 于淑晶, 郭宁, 等. 玉米茎基腐病防治研究进展[J]. 农药, 2021, 60(4): 235-238.
[6] 段灿星, 曹言勇, 董怀玉, 等. 玉米种质资源抗腐霉茎腐病和镰孢茎腐病精准鉴定[J]. 中国农业科学, 2022, 55(2): 265-279.
[7] 郑俊强, 高增贵, 庄敬华, 等. 玉米土传病害生物防治的研究进展[J]. 玉米科学, 2005, 13(1): 111-114, 118.
[8] Saravanakumar K, Fan L, Fu K, et al. Cellulase from Trichoderma harzianum interacts with roots and triggers induced systemic resistance to foliar disease in maize[J]. Scientific Reports, 2016, 6: 35543.
[9] Lu Z X, Tu G P, Zhang T L. et al. Screening of antagonistic Trichoderma strains and their application for controlling stalk rot in maize[J]. Journal of Integrative Agriculture, 2020, 19(1): 145-152.
[10] 崔文艳, 何朋杰, 尚娟, 等. 解淀粉芽孢杆菌B9601-Y2对玉米的防病促生长效果研究[J]. 玉米科学, 2015, 23(5): 153-158.
[11] 方中达. 植病研究方法[M]. 北京: 中国农业出版社, 1998: 192-195.
[12] 林玲, 陈怀谷, 刘磊, 等. 小麦纹枯病菌拮抗细菌的筛选及生物活性测定[J]. 江苏农业学报, 2003(3): 187-188.
[13] 连彩, 郭晓军, 朱宝成, 等. 兰花枯萎病拮抗细菌的筛选与鉴定[J]. 华北农学报, 2012, 27(2): 222-225.
[14] 中国科学院微生物研究所放线菌分类组. 链霉菌鉴定手册[M]. 北京: 科学出版社, 1975, 17-66
[15] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001, 349-388
[16] Lane D J. 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M, eds. Nucleic acid techniques in bacterial systematics[M]. Chichester: John Wiley & Sons, 1991, 115-175.
[17] 张涵, 习慧君, 赵玉华, 等. 杨浦链霉菌WLU210的筛选、鉴定及其对苦瓜枯萎病的生防作用研究[J]. 中国生物防治学报, 2021, 37(05): 1058-1065.
[18] 赖宝春, 戴瑞卿, 吴振强, 等. 链霉菌XG40的鉴定及其对琯溪蜜柚黑斑病的防效[J]. 中国生物防治学报, 2022, 38(3): 662-672.
[19] 赵清爽. 玉米镰刀菌穗腐病接种方法及品种抗性鉴定研究[D]. 河南: 河南农业大学, 2018.
[20] 杨克泽, 马金慧, 吴之涛, 等. 可溶性硅和杀菌剂对禾谷镰刀活性影响及田间药效试验[J]. 玉米科学, 2022, 30(04): 172-178, 183.
[21] Acimovic S G, Zeng Q, Mcghee G C, et al. Control of fire blight (Erwinia amylovora) on apple trees with trunk-injected plant resistance inducers and antibiotics and assessment of induction of pathogenesis-related protein genes[J]. Frontiers in Plant Science, 2015, 6: 16.
[22] Kloepper J W, Beauchamp C J. A review of issues related to measuring colonization of plant roots by bacteria[J]. Canadian Journal of Microbiology, 1992, 38(12): 1219-1232.
[23] 杨蓉, 杨文琦, 张峥, 等. 2株番茄早疫病拮抗菌的分离筛选与拮抗作用研究[J]. 新疆农业科学, 2017, 54(8): 1496-1504.
[24] 李玉洋, 辛寒晓, 范学明, 等. 水稻恶苗病拮抗菌的筛选、鉴定及其抑菌活性[J]. 生物技术通报, 2017, 33(5): 190-196.
[25] 张思奇, 孙丽萍, 赵同雪, 等. 玉米茎基腐病生防菌的筛选及应用[J]. 微生物学通报, 2017, 44(10): 2345-2352.