Screening, Identification and Control Efficacy of Biocontrol Strain against Purple Blotch of Garlic

doi:10.16409/j.cnki.2095-039x.2023.02.015

Abstract

Abstract: Purple blotch is one of the common diseases in various garlic-growing areas in Jiangsu, China. It causes huge economic losses and affects the sustainable development of the garlic industry. At present, the prevention of garlic purple blotch is mainly dependent on chemical control, while biological control research is limited. To improve the prevention level of garlic purple blotch, the biocontrol strains with clear antagonistic effect against garlic purple blotch pathogen (Alternaria alternata) were selected, and the control efficacy was evaluated with pot experiment and field trial. Among the 122 bacterial strains isolated from the soil of garlic fields, 61 strains were antagonistic to A. alternata, and the 12 strains with the strongest antagonistic ability were analyzed in more detail. Pot experiment and field trial showed that the JS51 and JS62 had the highest control efficacy against garlic purple blotch, and their control efficacies reached 61.63% and 55.05%, respectively, which was equivalent to that of prochloraz-manganese chloride complex. Based on morphological observations and molecular analyses of 16S rRNA and rpoB gene, JS51 and JS62 strains was identified as Bacillus velezensis. These strains have the potential to be developed into environmentally friendly biological pesticides in the future.

Key words: Bacillus velezensis, purple blotch of garlic, screening and identification, Alternaria alternata, control efficacy

CLC Number:

S476

LI Jiaxuan, MA Jinjun, ZHOU Dongmei, DENG Sheng, FENG Hui, WEI Lihui, WANG Xiaoyu. Screening, Identification and Control Efficacy of Biocontrol Strain against Purple Blotch of Garlic[J]. Chinese Journal of Biological Control, 2023, 39(2): 389-396.

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