Identification of Two Strains of Bacillus velezensis Isolated from Carya illinoensis Leaf and Their Antagonistic Effects on Pecan Black Spot Pathogen

doi:10.16409/j.cnki.2095-039x.2022.08.003

Abstract

Abstract: Black spot is an important pathogenic fungi disease infected pecan (Carya illinoensis). To develop green and efficient biological control method for pecan black spot disease, effective antagonistic endophytic strains were screened and identified as biocontrol resources against black spot disease. Based on morphological identification, physiological and biochemical characteristics, and phylogenetic analysis of 16S rDNA and GyrA gene, entophytic antagonistic strains isolated from pecan leaf were identified and their inhibition effects on mycelium growth of 26 isolates fungi caused black spot disease was detected by plate confrontation method. Two antagonistic entophytic strains screened from pecan leaf, named ZJJDZY and ZJJDYB, were identified as Bacillus velezensis. Both ZJJDZY and ZJJDYB showed significant antagonistic effects on 12 Colletotrichum species, 1 Botryosphaeria species, 2 Alternaria species, 4 Neopestalotiopsis species and 1 Phomopsis species. ZJJDZY and ZJJDYB demonstrated higher inhibition activity against PCZJJD18 C. henanense, PCAHFY5 C. sojae, PCSCLZ2 N. saprophytica, PCAHFY6 A. alternate and PCZJJD14 Phomopsis sp., with the inhibitory rates ranging from 57.1% to 60.6%, and the antibacterial ability of ZJJDZY and ZJJDYB was similar. Inhibition effects of ZJJDZY and ZJJDYB against different pathogenic fungi varied significantly at different concentrations, and the best antagonistic effect was shown at the concentration of 10⁸ CFU/mL. While, with the decrease of concentration, the antagonistic effect weakened significantly. Two entophytic antagonistic bacteria, ZJJDZY and ZJJDYB showed significant antagonistic effects against various pathogenic fungi causing black spot disease, which has the potential as biocontrol resource.

Key words: pecan, black spot disease, identification, Bacillus velezensis, biological control

CLC Number:

S476

WANG Di, GAO Yan, WU Xiaoshuang, ZHANG Wei, SHU Jinping, ZHANG Yabo, ZHAI Fengyan. Identification of Two Strains of Bacillus velezensis Isolated from Carya illinoensis Leaf and Their Antagonistic Effects on Pecan Black Spot Pathogen[J]. Chinese Journal of Biological Control, 2022, 38(6): 1572-1581.

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