Screening and Identification of Broad-Spectrum Antagonistic Trichoderma against Pathogenic Fungi for Apple and Its Field Control Effect in Orchard

doi:10.16409/j.cnki.2095-039x.2023.02.019

Abstract

Abstract: In order to screen broad-spectrum fungal antagonist against apple pathogenic fungi for apple, two Trichoderma strains YTLY-13 and YTLY-36, which had obvious antagonistic effects against four common pathogenic fungi for apple, were isolated from orchards in a variety of places in Shandong Province via plate confrontation method and growth rate method. The results indicated that YTLY-13 and YTLY-36 were Trichoderma virens and Trichoderma harzianum respectively, based on their morphological characteristics and sequence homology of translation elongation factor 1 (TEF1)，which had obvious inhibitory effect on the growth of pathogenic fungi, with the inhibition rate of 68.45%, among which the inhibition rate against Cytospora mandshurica was relatively high, reaching 73.92%. The results of field efficacy showed that the control effect of strain YTLY-36 against four fungal diseases of apple was significantly higher than that of strain YTLY-13, which was equivalent to the registered agent metiram and Bacillus subtilis, and had fast growth rate and simple fermentation, and YTLY-36 exhibited the good potential to be developed as a special broad-spectrum microbial fungicide for apple trees.

Key words: apple, fungal diseases, antagonists, identification, Trichoderma spp., field efficacy

CLC Number:

S476

ZHAO Yunfu, QIAO Shuqin, WANG Haiyan, XU Haijun, ZHAO Lihua, WU Haibin, SUN Zhiqiang, WANG Jianing, LIANG Chen, b. Screening and Identification of Broad-Spectrum Antagonistic Trichoderma against Pathogenic Fungi for Apple and Its Field Control Effect in Orchard[J]. Chinese Journal of Biological Control, 2023, 39(3): 567-574.

References

[1] 王玮, 王胜永, 李德海, 等. 江苏省丰县苹果生产绿色防控现状调查与分析[J]. 农学学报, 2021, 11(10):41-47.
[2] 王树桐, 王亚南, 曹克强, 等. 近年我国重要苹果病害发生概况及研究进展[J]. 植物保护, 2018, 44(5):13-2550.
[3] 阮盈盈, 刘峰. 木霉菌生物防治作用机制与应用研究进展[J]. 浙江农业科学, 2020, 61(11):2290-2294.
[4] 韩长志. 植物病原拮抗菌木霉属真菌的研究进展[J]. 江苏农业学报, 2016, 32(4):946-952.
[5] Harman G E. Myths and dogmas of biocontrolchanges inperceptions derived from research on Trichoderma harzinum T-22[J]. Plant Disease, 2000, 84(4):377-393.
[6] Rincon T, Limon A M, Condon M C, et al. Biocontrol mechanisms of Trichoderma strains.[J]. International Microbiology, 2004, 7(4):249-260.
[7] Vinale F, Sivasithamparam K, Ghisalberti E L. Trichoderma-plant-pathogen interactions[J]. Soil Biology and Biochemistry, 2008, 40(1):1-10.
[8] Sylla J, Alsanius B W, Krugerl E. Leaf microbiota of strawberries as affected by biological control agents[J]. Phytopathology, 2013, 103(10):1001.
[9] Arzanlou M, Khodaei S, Narmani A. Inhibitory effect of Trichoderma isolates on growth of Alternaria alternata, the causal agent of leaf spot disease on sunflower, under laboratory conditions[J]. Archiv Fr Pflanzenschutz, 2014, 47(13):1592-1599.
[10] Ferrigo D, Raiola A, Piccolo E, et al. Trichoderma harzianum T22 induces in maize systemic resistance against Fusarium verticillioides[J]. Journal of Plant Pathology, 2014, 96(1):133-142.
[11] Harman S. The molecular basis of shoot responses of maize seedlings to Trichodertna harzianum T22 inoculation of the root:a proteomic approach[J]. Plant Physiology, 2008, 147(4):2147-2163.
[12] Utkhede R S, Smith E M. Promotion of apple tree growth and fruit production by the EBW-4 strain of Bacillus subtilis in apple replant disease soil[J]. Canadian Journal of Microbiology, 1993, 38(12):1270-1273.
[13] 袁雪, 王珊珊, 赵晓萌, 等. 苹果腐烂病病原菌的鉴定及拮抗菌筛选[J]. 中国农学通报, 2017, 33(1):33-37.
[14] 赵璐, 刘胜, 胡同乐, 等. 复合微生物菌剂对苹果再植病害的生防效果测定及拮抗菌株鉴定[J]. 植物保护学报, 2019, 46(1):208-215.
[15] 许圆圆, 宁娜, 李发康, 等. 2种生防菌对苹果树3种病害病原菌拮抗作用比较[J]. 甘肃农业科技, 2018(11):24-29.
[16] 于晓丽, 王培松, 李宝燕, 等. 果树真菌病害拮抗细菌的筛选、鉴定及拮抗机制初探[J]. 果树学报, 2016, 33(6):734-743.
[17] 沈萍, 陈向东. 微生物学实验[M]. 第4版. 北京:高等教育出版社, 2007.
[18] 周畅, 王金杰, 杨广琦, 等. 稠李根围木霉的分离及鉴定[J]. 北方园艺, 2018(12):88-95.
[19] 尉莹莹, 王佳宁, 梁晨, 等. 番茄晚疫病菌拮抗木霉菌株的筛选与鉴定[C]//植物病理学研究进展-中国植物病理学会第十二届青年学术研讨会论文选编, 2015:16-23.
[20] 杨立宾, 宋瑞清, 邓勋, 等. 致病疫霉生防木霉菌株筛选鉴定及生物学特性[J]. 东北林业大学学报, 2013(7):118-128.
[21] 张广志, 杨合同, 文成敬. 木霉菌形态学分类检索与分子生物学鉴定[J]. 山东农业大学学报, 2011, 42(2):309-316.
[22] 王勇, 王万立, 霍建飞, 等. 绿色木霉基因组DNA不同提取方法的比较研究[J]. 山东农业科学, 2012, 44(5):14-16.
[23] 杨春平, 张晋康, 陈华保, 等. 绿色木霉L24菌株分生孢子可湿性粉剂的研制[J]. 西北农业学报, 2010(19):43-47.
[24] 周畅. 新疆野苹果病害防治专用木霉可湿性粉剂剂型制备及药效检测[D]. 哈尔滨:东北林业大学.
[25] 农业部农药检定所生测室主编. 农药田间药效试验准则(二)[M]. 北京:中国标准出版社, 2000.
[26] 罗俊彩, 扈进冬, 李纪顺, 等. 分子生物学方法在木霉菌分类研究中的应用[J]. 山东科学, 2010, 23(4):19-21
[27] 张广志, 张新建, 陈泉, 等. 哈茨木霉复合种内3个中国新记录种的分离和鉴定[J]. 山东科学, 2015, 28(6):4643-4651.
[28] Chaverri P, Samuels G J, Stewart E L. Hypocrea virens sp. nov. the teleomorph of Trichoderma virens[J]. Mycologia, 2001, 93:1113-1124.
[29] 张文军, 毛维兴, 张树武, 等. 深绿木霉T2菌株对苹果霉心病的防治效果研究[J]. 中国果树, 2018(5):11-14.
[30] 任凤山, 王燕, 翟一凡, 等. 木霉与几种化学杀菌剂协同防治苹果轮纹病[J]. 北方园艺, 2015(16):111-115.
[31] 田桢, 曹传旺, 季世达, 等. 新疆野果林木霉分离鉴定、抑菌作用及固态发酵配方优化[J]. 微生物学通报, 2021, 48(12):4696-4709.
[32] 张莉莉, 刘美媛, 王永章, 等. 木霉菌对苹果主要真菌病害的抑制及其在土壤中的定殖作用[J]. 北方园艺, 2021(6):15-22.