Study on the Function of ecp Gene Cluster in Colonization of Pine Wood Nematode by Enterobacter Ludwigii AA4

doi:10.16409/j.cnki.2095-039x.2024.01.006

Abstract

Abstract: Enterobacter ludwigii AA4, a biocontrol bacterial strain, can efficiently colonize and kill pine wood nematode (PWN) to prevent the occurrence of pine wilt disease (PWD). Escherichia coli common pilus (ECP) can promote bacterial colonization by enhancing the bacteria-host interactions and bacterial interspecies interplay within the biofilms. The ECP is encoded by the ecp gene cluster. In order to explore the function of ECP in colonization of PWN by strain AA4, we identified the ecp gene cluster in AA4 by using BLAST alignment. By employing the Red (λ, β, exo)-mediated DNA homologous recombination technology, we further constructed the ecp gene cluster knock-out deletion mutant and analyzed its ability to colonize the PWN plus the colonization-related phenotypes. The outcomes showed that the pilus assembly, motility, biofilm formation of the knock-out mutant and its residence on the PWN was significantly reduced. Thus, ecp gene cluster may regulate the colonization of the PWN by E. ludwigii AA4 through affecting its motility and biofilm formation via modulation of its pilus synthesis. Altogether, our results will serve as theoretical bases for revealing the molecular mechanisms underlying the PWN colonization by strain AA4 and will provide scientific evidence guiding the development of biopesticides for controlling the PWD and their efficient application.

Key words: ecp gene cluster, pilus, Enterobacter ludwigii, pine wood nematode, colonization

CLC Number:

S476.2

WANG Mingyue, HE Shan, ZHANG Lixia, WANG Sijia, YUAN Zhibo, WU Di, WANG Chuanzhen, PAN Jialiang, BAO Shengbo, WANG Qi, NIU Ben. Study on the Function of ecp Gene Cluster in Colonization of Pine Wood Nematode by Enterobacter Ludwigii AA4[J]. Chinese Journal of Biological Control, 2024, 40(5): 1022-1035.

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