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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (5): 1223-1230.DOI: 10.16409/j.cnki.2095-039x.2022.06.010

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The Function of Bacillus velezensis 5YN8 Biofilm in Controlling of Botrytis cinerea in Tomato

SI Fangjie, REN Jinyao, HUANG Taoxiang, YU Yiyang, GUO Jianhua, JIANG Chunhao   

  1. College of plant protection, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2021-08-13 Published:2022-10-20

Abstract: Gray mold disease is one of the important soil borne diseases of tomato,which is caused by Botrytis cinerea.It endangers the fruit,leaf,stem and other parts of tomato and seriously restricts the safe production of tomato.In the present,the application of microbial agents is considered to be an effective measure to control this disease.Previously,it was found that Bacillus velezensis 5YN8 could significantly control gray mold disease in tomato.In this study,we constructed mutants of biofilm-related genes to explore the role of biofilm in the disease control.The results showed that the biofilm-induced mutants formed more robust biofilms with significant more wrinkles,while the biofilm-impaired mutants formed weaker biofilms with smooth surfaces and less wrinkles.Further results demonstrated that the colonization of the biofilm-impaired mutants on the leaf was significantly reduced 14 days after bacterial treatment,meanwhile,the leaf colonization of the biofilm-induced mutants was significantly increased.The disease control effect of biofilm-impaired mutant∆spo0A significantly reduced,with the efficacy of only 16.02%.On the contrary,the control effect of∆abrB and∆sinR significantly increased,with the efficacies of 80.77% and 78.94%,respectively.Correlation analysis indicated that there was a significant positive correlation between the inhibition zone,the bacterial colonization and the control efficacy of 5YN8.To sum up,it was found that the formation of 5YN8 biofilm depended on the regulation of conserved genes,and the control effect of B.cinerea in tomato was affected by colonization and antagonism of 5YN8.The above results will provide a theoretical basis for the functional modification and enhancement of the biocontrol agent 5YN8 and its application in B.cinerea.

Key words: gray mold disease, Bacillus velezensis, biological control, biofilm, colonization

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