Effects of Ammonia Water and Allyl Isothiocyanate Fumigation on Root-Knot Nematode and Microbial Community Response in Greenhouse Soil

doi:10.16409/j.cnki.2095-039x.2025.02.065

Abstract

Abstract: To identify an environmentally friendly soil fumigant with superior efficacy against root-knot nematodes, this study systematically evaluated the effects of ammonia water (AW) and allyl isothiocyanate (AITC) on nematode control, soil microbial community structure, and crop yield using Brassica rapa var. glabra Regel as a model crop. Control efficacy of AW and AITC on root-knot nematode was 81.38% and 72.12%, respectively, while increasing Brassica rapa var. glabra Regel yield by 15.53% and 8.97%. AW significantly enhanced plant height, root length, leaf length, and fresh weight, whereas AITC notably increased leaf width. Soil enzyme activity analysis indicated that AW inhibited urease and N-acetyl-β-D-glucosaminidase activities, while AITC reduced nitrate reductase activity. Microbial community analysis revealed that both AW and AITC treatments suppressed the abundance of bacterial genera Marmoricola (63.13% and 79.07%) and Terrimonas(71.59% and 80.11%), as well as the fungal genera Penicillium, Cercophora, and Blastobotrys. Additionally, the AITC treatment significantly reduced the relative abundance of the genus Peribacillus while increasing that of Caldicoprobacter. In summary, both AW and AITC possess distinct advantages in controlling root-knot nematodes and regulating soil ecology, providing a theoretical basis for the selection of green fumigants.

Key words: root-knot nematodes, ammonia water fumigation, allyl isothiocyanate fumigation, soil enzyme activity, microbial community

CLC Number:

S476

FU Jiawei, LI Pengyuan, YIN Fuhao, DING Jianli, ZHANG Shubin, ZHU Yingbo, LIU Jianbin. Effects of Ammonia Water and Allyl Isothiocyanate Fumigation on Root-Knot Nematode and Microbial Community Response in Greenhouse Soil[J]. Chinese Journal of Biological Control, 2025, 41(5): 1154-1165.

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