Isolation, Identification and Biocontrol Potential of Antagonistic Fungi against Zanthoxylum bungeanum Root Rot

doi:10.16409/j.cnki.2095-039x.2023.02.001

Abstract

Abstract: In order to obtain good antagonistic strains against Fusarium solani, the endophytic fungus of Zanthoxylum bungeanum root was screened by tissue isolation method. The strain HB21-1 with inhibition rate of 61.00% against F. solani was obtained by plate confrontation method. The inhibition rate of its sterile fermentation filtrate against F. solani was 41.24%. The strain of HB21-1 induced constriction, enlargement in mycelia morphology of F. solani and caused spore deformity. Based on the morphological characteristic and ITS molecular sequences, The strain of HB21-1 was identified as Penicillium oxalicum. The effect of HB21-1 on root rot of Zanthoxylum bungeanum was 56.59% in the pot experiment. The POD and SOD enzyme activities, soil sucrase and acid phosphatase activities of Zanthoxylum bungeanum roots treated with HB21-1 were increased compared with the control. This study shows that P. oxalicum HB21-1 had a significant antagonistic effect on F. solani and reduced the incidence of the root rot of Zanthoxylum bungeanum.

Key words: Zanthoxylum bungeanum, root rot, Penicillium oxalicum, biological control, defense enzyme activity, soil enzyme activity

CLC Number:

S476

LI Mengwei, CHEN Xiaoxia, BU Huanhuan, LIAO Libin, LI Jingyi, ZHANG Nannan, SHI Fusun. Isolation, Identification and Biocontrol Potential of Antagonistic Fungi against Zanthoxylum bungeanum Root Rot[J]. Chinese Journal of Biological Control, 2023, 39(1): 176-183.

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