Biocontrol Potential of Bacillus subtilis GXHZ16 against Tobacco Black Shank

doi:10.16409/j.cnki.2095-039x.2025.02.078

Abstract

Abstract: To clarify biocontrol potential of antagonistic bacterium GXHZ16 against tobacco black shank, the strain was first identified based on its morphological characteristics, physiological and biochemical properties, and sequence analyses of the 16S rRNA and gyrB genes. Subsequently, pot experiments were conducted to evaluate tits control efficacy against tobacco black shank. Furthermore, hyphal morphology of Phytophthora nicotianae after exposure to GXHZ16 was observed using scanning electron microscopy (SEM), antimicrobial activity of crude lipopeptides from GXHZ16 was assessed through mycelial growth rate assays, and PCR detection was used to identify genes encoding lipopeptides. Then, the labelled strain GXHZ16-gfp was obtained through the green fluorescent protein (gfp) tagging, and its colonization dynamics in rhizosphere soil and roots of tobacco plants were investigated. The results showed GXHZ16, identified as Bacillus subtilis, exhibited a control efficacy of 52.21% against tobacco black shank, and caused abnormal hyphal morphology of P. nicotianae. The crude lipopeptides of GXHZ16 strongly inhibited the growth of P. nicotianae, and GXHZ16 was found to contain genes encoding lipopeptides such as plipstatin, fengycin, and surfactin. In addition, GXHZ16 stably colonized the tobacco rhizosphere soil and roots, with colonization densities of 25.20× 10⁶ and 12.93× 10⁵ cfu/g, respectively, on the 28th day after inoculation. These findings provided a theoretical foundation for the further exploration and industrial development of GXHZ16.

Key words: Phytophthora nicotianae, Bacillus subtilis, lipopetide, colonization, biocontrol potential

CLC Number:

S476

PENG Yu, HU Yajie, WU Huiping, FAN Qiling, LI Caibin, TANG Ai, CHEN Dehui, LI Xiang, CHEN Jianguo, DING Ting, WU Feng. Biocontrol Potential of Bacillus subtilis GXHZ16 against Tobacco Black Shank[J]. Chinese Journal of Biological Control, 2025, 41(6): 1439-1451.

References

[1] 李彩斌, 程云龙, 何轶, 等. 烟草黑胫病菌高效杀菌剂的筛选[J]. 烟草科技, 2023, 56(6): 34-39.
[2] Guo D, Yuan C, Luo Y, et al. Biocontrol of tobacco black shank disease (Phytophthora nicotianae) by Bacillus velezensis Ba168[J]. Pesticide Biochemistry and Physiology, 2020, 165: 104523.
[3] 刘迎龙, 王军伟, 陆俊平, 等. 云南玉溪烟区烟草黑胫病菌的生理小种鉴定及其致病性分析[J]. 烟草科技, 2023, 56(6): 17-23.
[4] 李建军, 许世洋, 马佳勇, 等. 一种根腐类病害防病促生复合微生物菌系的优化及其效应[J]. 微生物学通报, 2025, 52(3): 1118-1133.
[5] 刘艳霞, 陶正朋, 李想, 等. 抗青枯病型根际促生菌(PGPR)菌群构建及其生物防控机制[J]. 微生物学报, 2023, 63(3): 1099-1114.
[6] 郭庆港, 刘高鸽, 陈秀叶, 等. 枯草芽胞杆菌HMB19198菌株抑菌物质的鉴定及其对番茄灰霉病的防治[J]. 植物病理学报, 2022, 52(2): 247-255.
[7] 贾孟媛, 王越洋, 唐培培, 等. 烟草黑胫病生防菌的筛选鉴定及其防效[J]. 湖南农业大学学报(自然科学版), 2023, 49(3): 329-334.
[8] 孙亦婷, 郝浩浩, 唐培培, 等. 菌株SYT-SF的筛选鉴定及生防效果验证[J]. 现代农业科技, 2023(10): 89-92, 96.
[9] 常栋, 顾建国, 贾方方, 等. 烟草黑胫病不同植物源生防菌的筛选及防效测定[J]. 中国烟草学报, 2020, 26(3): 84-90.
[10] Qiu Y, Yan H H, Sun S M, et al. Use of Bacillus velezensis SDTB022 against tobacco black shank (TBS) and the biochemical mechanism involved[J]. Biological Control, 2022, 165: 104785.
[11] 高强, 张晓阳, 张渐隆, 等. 生防菌CY2的筛选鉴定及其与氟噻唑吡乙酮复配防治烟草黑胫病的效果[J]. 烟草科技, 2025, 58(3):70-79.
[12] 危潇, 黎妍妍, 姚经武, 等. 哈茨木霉WF2菌株鉴定及对烟草黑胫病的防效[J]. 中南农业科技, 2023, 44(11): 12-15.
[13] 隋宗明, 袁玲. 撕裂蜡孔菌对烟草黑胫病的防治效果及对烤烟生长的影响[J]. 植物保护学报, 2019, 46(5): 1138-1146.
[14] 曾祥难, 刘新源, 彭博, 等. 烟草疫霉拮抗菌的分离鉴定及其生防和发酵特性[J]. 江苏农业科学, 2024, 52(16): 161-170.
[15] 李亚楠, 张豪杰, 石晖琴, 等. 马铃薯炭疽病生防芽胞杆菌QB-2-7和 BM-7的鉴定及其合成菌群的生防作用[J]. 农业生物技术学报, 2025, 33(11): 2441-2459.
[16] 胡亚杰, 范啟铃, 李群岭, 等. 枯草芽胞杆菌GXHZ16抑制烟草疫霉病菌的表达谱分析[J]. 中国生物防治学报, 2024, 40(3): 608-619.
[17] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001.
[18] 张娜娜, 徐琼, 黎鸿艺. 腐乳中菌群基因组DNA提取方法的对比研究[J].中国酿造, 2023, 42(10): 177-181.
[19] 吴晓东, 范存忠, 张志斌, 等. 蛇足石杉内生细菌的分离鉴定及其促生特性[J]. 微生物学通报, 2025, 52(6): 2570-2587.
[20] 崔皓玥, 田露, 胡乐梅, 等. 解淀粉芽孢杆菌wxy-b3产脂肽类物质对苹果斑点落叶病菌的抑菌活性研究[J]. 陕西科技大学学报, 2025, 43(1): 46-51.
[21] 刘妮, 王印庚, 于永翔, 等. 枯草芽孢杆菌斯氏亚种胞外产物的抑菌活性及其特性分析[J]. 微生物学报, 2024, 64(8): 2967-2985.
[22] 蔡露, 秦艳秋, 高坦坦, 等. 枯草芽孢杆菌菌株ZT4-2电击转化体系的建立[J]. 北京农学院学报, 2022, 37(1): 32-36.
[23] Ding H, Mo W, Yu S, et al. Whole genome sequence of Bacillus velezensis strain GUMT319: a potential biocontrol agent against tobacco black shank disease[J]. Frontiers in Microbiology, 2021(12): 658113.
[24] Wu G, Liu Y, Xu Y, et al. Exploring elicitors of the beneficial rhizobacterium Bacillus amyloliquefaciens SQR9 to induce plant systemic resistance and their interactions with plant signaling pathways[J]. Molecular Plant-Microbe Interactions, 2018, 31(5): 560-567.
[25] Perea-Molina P A, Pedraza-Herrera L A, Beauregard P B, et al. A biocontrol Bacillus velezensis strain decreases pathogen Burkholderia glumae population and occupies a similar niche in rice plants[J]. Biological Control, 2022, 176:105067.
[26] 宋晓雅, 毛子玥, 温德宇, 等. 番茄灰霉病生防细菌QZ-1生防潜力研究[J]. 扬州大学学报(农业与生命科学版), 2024, 45(2): 93-101.
[27] 罗雨晴, 盛浩, 袁红, 等. 多粘类芽孢杆菌LRS-1诱导黄瓜抗疫霉病的苯丙烷类代谢基因表达与调控研究[J]. 西南农业学报, 2020, 33(10): 2262-2266.