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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (5): 714-720.DOI: 10.16409/j.cnki.2095-039x.2020.06.002

• RESEARCH REPORTS • Previous Articles     Next Articles

The Effect of Biochar on Anti-ultraviolet Ability of Cry1Ac Protein

RAO Wenhua, ZHAN Yating, FANG Yun, YOU Yuxin, NIE Danyue, GUO Xueping, ZHANG Dingyang, GUAN Xiong, PAN Xiaohong   

  1. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University/Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fuzhou 350002, China
  • Received:2020-06-02 Online:2020-10-08 Published:2020-11-20

Abstract: In order to study the protective effect of biochar on ultraviolet, we made biochar from bean shell as the carrier, and the adsorption behavior of biochar on Bt Cry1Ac protein and the UV protection of biochar on Cry1Ac protein were studied. The morphology and structure of biochar were characterized by SEM, TEM, XRD and FTIR. The results showed that biochar is a typical porous material with abundant functional groups on its surface. The adsorption equilibrium time of Cry1Ac protein and biochar was 50 min, and the most suitable adsorption concentration ratio (biochar:protein) was 1:100. The adsorption of Cry1Ac protein and biochar conformed to the quasi second-order kinetic model and Langmuir model. After UV irradiation for 4 hours, the biological activity of biochar and Cry1Ac protein complex on Helicoverpa armigera was 4.93 times higher than that of pure protein, which showed that biochar had better UV resistance ability. The results show that biochar could significantly improve the anti-UV ability of Cry1Ac protein, which provide new materials for development of UV resistant pesticide formulation.

Key words: Bacillus thuringiensis, Cry1Ac protein, biochar, adsorption, UV resistance

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