Screening and Identification of Antagonistic Fungal against Tobacco Fusarium oxysporum and Its Growth Promotion Effect

doi:10.16409/j.cnki.2095-039x.2021.06.008

Abstract

Abstract: In order to obtain the antagonistic fungal strains against tobacco Fusarium root rot, 371 fungal strains were isolated from the tobacco rhizosphere soil in the tobacco area of Henan Province by dilution coating plate method. One Trichoderma strain with obvious inhibitory effect against F. oxysporum was obtained by the plate confrontation culture method, and was identified as T. asperellum Tr-0111 by morphological and molecular biology methods. Tr-0111 strain had a maximum inhibitory rate of 93.13% against F. oxysporum, and also had a strong inhibitory effect on 8 other tobacco pathogens, such as Thielaviopsis basicola, Phomopsis sp., Alternaria alternata, etc. The growth-promoting effect test showed that the fermentation broth of the strain increased the germination rate of tobacco seeds by 5.34%, and the increase rate of root length of tobacco seedlings was 62.39%. It also had a significant growth-promoting effect on the maximum leaf area and fresh weight of the above-ground part of potted tobacco. The results laid the foundation for further research on the related mechanisms and development of biocontrol agents.

Key words: Tobacco Fusarium root rot, Fusarium oxysporum, Trichoderma asperellum, inhibitory effect, growth promotion

CLC Number:

S476

YAO Chenxiao, LI Xiaojie, LI Qi, QIU Rui, BAI Jingke, LIU Chang, CHEN Yuguo, KANG Yebin, LI Shujun. Screening and Identification of Antagonistic Fungal against Tobacco Fusarium oxysporum and Its Growth Promotion Effect[J]. Chinese Journal of Biological Control, 2021, 37(5): 1066-1072.

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