Screening of the Inhibitory Effect of Antagonistic Bacteria Compounded with Herbal Extracts on Sphaeropsis sapinea

doi:10.16409/j.cnki.2095-039x.2024.02.069

Abstract

Abstract: Pine wilt is a worldwide pine disease caused by Sphaeropsis sapinea,The main control methods are chemical control and biological control. Biological control is favored because it is based on nature and has the inherent advantages of green environmental protection. In order to screen effective antagonistic microorganisms, a strain of Bacillus amyloliquefaciens CSUFT-CSBA69 was selected from rhizosphere soil of Pinus massoniana. The inhibition rate was 76.18%. The crude extracts of traditional Chinese medicine were tested and their toxicity was determined. The results showed that Coptis Chinensis had the best inhibitory effect. The EC₅₀ value was 0.033 mg/mL, the MIC was 0.3125 mg/mL, the MBC was 0.625 mg/mL, and the bacteriostatic effect was still present when the concentration decreased to 0.0097 mg/mL. In order to solve the realistic problem of the unstable effect of a single biocontrol factor in production practice and meet the demand of reducing the cost of biocontrol agents, this study adopted the compound method to explore the synergistic control effect of strain CSUFT-CSBA69 and Coptis chinensis low-concentration extract on S. sapinea. The bacterial inhibition was 97.45% when the Coptis chinensis was 0.05mg/mL and the inoculated amount of aseptic filtrate was 10%. The study demonstrated that the extract of Coptis Chinensis has a good inhibitory effect on pathogenic bacteria and could be combined with biocontrol bacteria. This study provided a new idea for the preparation of bio-derived pesticides.

Key words: Sphaeropsis sapinea, Bacillus amyloliquefaciens, plant extracts, compound, biological control

CLC Number:

S476

NIU Anqi, ZHOU Deming, MIAO Xinyu, CHEN Xinggang, ZHOU Guoying, LIU Junang. Screening of the Inhibitory Effect of Antagonistic Bacteria Compounded with Herbal Extracts on Sphaeropsis sapinea[J]. Chinese Journal of Biological Control, 2024, 40(6): 1355-1365.

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