Biocontrol Effects of Bacillus amyloliquefaciens HN11 in Combination with Tobacco Waste Organic Fertilizers on Strawberry Root Rot

doi:10.16409/j.cnki.2095-039x.2025.02.009

Abstract

Abstract: This study investigates an environmentally friendly approach to control strawberry root rot using biocontrol agents. Laboratory experiments revealed that B. amyloliquefaciens HN11 exhibits antagonistic activity against various soil-borne pathogens, particularly disrupting the mycelial structure of F. oxysporum, the primary pathogen causing strawberry root rot, and interfering with its physiological metabolism. In pot experiments, the combination of HN11 and tobacco waste organic fertilizer achieved a disease control efficacy of 75.00% against strawberry root rot. The organic fertilizer enhanced the colonization ability of HN11, maintaining its population at 10⁴-10⁵ CFU/g in soil and rhizosphere even 30 days after application. Field trials further demonstrated that applying HN11 via root drench at 75 L/h² in conjunction with 6000 kg/hm² of tobacco waste organic fertilizer resulted in the best disease control efficacy of 73.85%, while also promoting strawberry growth. High-throughput sequencing analysis indicated that this combined application optimized the soil microbial community structure, increasing the relative abundance of beneficial genera such as Bacillus and Pseudomonas, while reducing the relative abundance of Ascomycota. Overall, the integration of HN11 with tobacco waste organic fertilizer offers a promising green strategy for the management of strawberry root rot.

Key words: Bacillus amyloliquefaciens, tobacco waste organic fertilizer, strawberry root rot, biological control, soil microorganisms

CLC Number:

S432
S476

LIU Miaojiao, LIN Sukun, LIU Rong, ZHANG Junhui, LI Lin, YI Ting, XU Hanhong, ZENG Lingda. Biocontrol Effects of Bacillus amyloliquefaciens HN11 in Combination with Tobacco Waste Organic Fertilizers on Strawberry Root Rot[J]. Chinese Journal of Biological Control, 2025, 41(1): 143-155.

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