Control Effect of Composite Microbial Consortium on Astragalus membranaceus Root Rot and Its Influence on Microorganism Community in Rhizosphere Soil

doi:10.16409/j.cnki.2095-039x.2025.02.068

Abstract

Abstract: To screen composite microbial consortium for the control of Astragalus membranaceus root rot, antagonistic strains were screened from the rhizosphere soil of A. membranaceus using the gradient dilution and plate confrontation methods. The strains were further identified based on morphological, physiological and biochemical characteristics and molecular biology methods. The antagonistic strains were combined to construct a consortium, and its effects on defense related enzymes in A. membranaceus roots were evaluated. The influence of the composite microbial consortium on soil bacterial community was analyzed using high-throughput sequencing technology. The results showed that strains 6-35, 17-27 and 62-313 could significantly inhibit Fusarium oxysporum, with the inhibition zone width of 6.5, 8.4 and 4.2 mm, respectively. Strains 6-35, 17-27 and 62-313 were identified as Bacillus amyloliquefaciens, Paenibacillus polymyxa and B. cereus, respectively. The optimal fermentation ratio was 3:1:1, with an inhibition rate of 40.90%. The composite microbial consortium showed the control efficacy of 72.17% and 80.36% at 7 and 14 d after inoculation on A. membranaceus root rot, respectively. After treatment with the composite microbial consortium, the activities of chitinase, peroxidase (POD), superoxide dismutase (SOD), phenylalanine ammonialyase (PAL), andcatalase (CAT) significantly increased in A. membranaceus roots. The application of composite microbial consortium could increase α and β diversity of rhizosphere bacterial communities, and various beneficial microorganisms such as Gemmatimonas and Streptomyces in the treatment soil was significantly higher than that in the control soil. The composite microbial consortium had excellent control effects on A. membranaceus root rot, and might be used for the development and application of biological control reagent.

Key words: antagonistic bacterium, identification, composite microbial consortium, defense enzyme, microbial diversity

CLC Number:

S476

WANG Yan, ZHAO Rui, GAO Yuqing, LI Jiangbo, WANG Chunwei. Control Effect of Composite Microbial Consortium on Astragalus membranaceus Root Rot and Its Influence on Microorganism Community in Rhizosphere Soil[J]. Chinese Journal of Biological Control, 2026, 42(1): 10-22.

References

[1] 白晓丽. 黄芪根系人工菌群构建及其对根腐病的防治潜力初探[D]. 咸阳: 西北农林科技大学, 2020.
[2] 汪天娜. 山核桃根腐病防治药剂筛选及应用研究[D]. 杭州: 浙江农林大学, 2021.
[3] 高芬, 任小霞, 王梦亮, 等. 中草药根腐病及其微生物防治研究进展[J]. 中国中药杂志, 2015, 40(21): 4122-4126.
[4] 赵丽梅, 赵利, 田洪岭, 等. 黄芪根腐致病尖孢镰刀菌实时荧光定量PCR检测体系的建立及应用[J]. 农业生物技术学报, 2024, 32(3): 720-729.
[5] 郑旭蕊. 复合微生物菌剂的研制及其对尖孢镰刀菌防治效果研究[D]. 哈尔滨: 哈尔滨工业大学, 2021.
[6] 王瑶, 赵月菊, 邢福国, 等. 禾谷镰刀菌拮抗菌株的筛选及鉴定[J]. 核农学报, 2017, 31(6): 1128-1136．
[7] 沙月霞, 曾庆超, 田兴芳, 等. 防治中药材根腐病芽胞杆菌的筛选及效果评价[J]. 中国生物防治学报, 2025, 41(3): 520-529.
[8] 谢学文, 赵昱榕, 孙雪莹, 等. 番茄疫霉根腐病生防菌的分离鉴定及其防治效果[J]. 植物病理学报, 2020, 50(5): 610-617.
[9] 张爱梅, 李曦冉, 郭保民, 等. 黄芪根腐病生防菌株的筛选鉴定及其防效评价[J]. 西北农业学报, 2021, 30(12): 1905-1913.
[10] 许磊磊, 白晓丽, 李哲斐. 黄芪根腐病拮抗菌的筛选及生防机制[J]. 西北师范大学学报(自然科学版), 2024, 60(2): 77-85.
[11] Defilippi S, Groulx E, Mmgalla M, et al. Fungal competitors affect production of antimicrobial lipopeptides in Bacillus subtilis strain B9-5[J]. Journal of Chemical Ecology, 2018, 44(4): 374-383.
[12] Li C, Hu W, Pan B, et al. Rhizobacterium Bacillus amyloliquefaciens strain SQRT3-mediated induced systemic resistance controls bacterial wilt of tomato[J]. Pedosphere, 2017, 27(6): 147-158.
[13] Zalila-kolsi I, Mahmoud A B, Ali H, et al. Antagonist effects of Bacillus spp. strains against Fusarium graminearum for protection of durum wheat (Triticum turgidum L. subsp. durum)[J]. Microbiological Research, 2016, 192: 148-158.
[14] 何礼远. 细菌在植物病害生物防治上应用研究的进展[J]. 生物防治通报, 1985, 3: 28-31.
[15] 李永刚, 宋兴舜, 马凤鸣, 等. 水稻稻瘟病拮抗菌L1鉴定及抑菌特性的初步研究[J]. 微生物学通报, 2008(6): 898-902.
[16] 王文丽, 金涵, 从炳成, 等. 复合微生物菌剂对番茄青枯病的生防效应[J]. 南京农业大学学报, 2022, 45(6): 1174-1182.
[17] Chen R, Dou J. Biofuels and bio-based chemicals from lignocellulose: Metabolic engineering strategies in strain development[J]. Biotechnology Letters, 2016, 38(2): 213-221.
[18] 苑宝洁, 李磊, 张红杰, 等. 黄瓜细菌性角斑病拮抗细菌的筛选及其防治效果[J]. 中国生物防治学报, 2022, 38(2): 421-427.
[19] 胡展, 付祖姣, 郭照辉, 等. 复合微生物菌剂对水稻稻瘟病的生防效应[J]. 微生物学通报, 2024, 51(2): 483-493.
[20] 布坎南, 吉本斯. 伯杰细菌鉴定手册[M]//中国科学院微生物研究所《伯杰细菌鉴定手册》翻译组, 译. 8版. 北京: 科学出版社, 1984.
[21] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001, 349-398.
[22] 王春伟, 王燕, 张曦倩, 等. 拮抗细菌菌株YJ15的分离鉴定、发酵条件优化及对越橘灰霉病的防效[J]. 园艺学报, 2018, 45(10): 42-53.
[23] 赵晓霞, 牛世全, 文娜, 等. 黄芪根腐病生防芽孢杆菌的筛选鉴定与盆栽防效试验[J]. 生物技术通报, 2019, 35(9): 107-111.
[24] 杨波, 李云翔, 徐瑾瑜, 等. 黄芪根腐病生防放线菌的筛选、鉴定及其应用[J]. 中国生物防治学报, 2025, 41(3): 554-560.
[25] 李亚楠, 张豪杰, 石晖琴, 等. 马铃薯炭疽病生防芽胞杆菌QB-2-7和 BM-7的鉴定及其合成菌群的生防作用[J]. 农业生物技术学报, 2025, 33(11): 2441-2459.
[26] 高艳东, 候可心, 李俊达, 等. 梭梭根际、内生细菌的促生作用及其复合菌群的构建[J/OL]. 微生物学通报, https://doi.org/10.13344/j.microbiol. china.250666.
[27] 郝锐. 黄芪根腐病拮抗芽孢杆菌鉴定及其生物农药开发潜力评价[D]. 太原: 山西大学, 2017.
[28] 樊良帅, 黄振斌, 晋小军, 等. 三种生物菌剂对黄芪根腐病防效及生长发育的影响[J]. 农药学学报, 2025, 27(1): 160-170.
[29] Bhusal B, Mmbaga M T. Biological control of Phytophthora blight and growth promotion in sweet pepper by Bacillus species[J]. Biological Control, 2020, 150: 104373.
[30] Nawaz K, Ali Shahid A, Bengyella L, et al. Diversity of Trichoderma species in chili rhizosphere that promote vigor and antagonism against virulent Phytophthora capsici[J]. Scientia Horticulture, 2018, 239: 242-252.
[31] 王琳, 陈雅如, 程湄婕, 等. 微生物几丁质酶研究进展及应用[J]. 中国生物工程杂志, 2022, 42(12): 101-110.
[32] 胡婷婷. 栽培基质对几种蔬菜有机生态型无土栽培的影响[D]. 延边: 延边大学, 2015.
[33] 杨梦珂, 郑思俊, 张青萍, 等. 应用于垂直绿化栽培的基质保肥性研究[J]. 河南农业科学, 2017, 46(11): 120-126.
[34] 邹芳斌, 司龙亭, 李新, 等. 黄瓜枯萎病抗性与防御系统几种酶活性关系的研究[J]. 华北农学报, 2008, 23(3): 181-184．
[35] 孙军利, 章智钧, 张坤, 等. 不同配方基质的理化性质及其对草莓生长的影响[J]. 河南农业科学, 2017, 46(3): 118-121, 128.
[36] 许勇, 王永健, 葛秀春, 等. 枯萎病菌诱导的结构抗性和相关酶活性的变化与西瓜枯萎病抗性的关系[J]. 果树科学, 2000, 17(2): 123-127.
[37] 蔡燕飞, 廖宗文, 章家恩, 等. 生态有机肥对蕃茄青枯病及土壤微生物多样性的研究[J]. 应用生态学报, 2003, 14(3): 349-353．
[38] Ellis R J, Morgan P, Weightman A J, et al. Cultivation dependent and independent approaches for determining bacterial diversity in heavy-metalcontaminated soil[J]. Applied and Environmental Microbiology, 2003, 69(6): 3223-3230．
[39] 刘丹, 张丽萍, 史延茂, 等. 生防细菌对植物根围微生态效应的研究进展[J]. 中国农学通报, 2014, 30(7): 260-265．
[40] 朱伟杰, 王楠, 郁雪平, 等. 生防菌Pseudomonas fluorescens 2P24对甜瓜根围土壤微生物的影响[J]. 中国农业科学, 2010, 43(7): 1389-1396．
[41] 范文艳, 陈瑾, 姜述君, 等. 复合生防菌群对连作大豆根际土壤可培养微生物区系的影响[J]. 中国油料作物学报, 2012, 34(3): 286-293．