Genome Sequencing of Paenibacillus polymyxa Pp146 and Evaluation of Its Growth-Promoting and Disease Control Effects on Medicinal Plants

doi:10.16409/j.cnki.2095-039x.2026.02.023

Abstract

Abstract: To systematically elucidate the plant growth-promoting and disease control characteristics of Paenibacillus polymyxa Pp146 and assess its application potential in medicinal plants, this study conducted a systematic analysis combining whole-genome sequencing with plate confrontation and pot experiments. Whole-genome sequencing revealed a genome size of 4925561 bp with a GC content of 46.84%. A total of 4826 protein-coding genes were identified, along with 14 5S rRNA genes, 13 16S rRNA genes, 13 23S rRNA genes, 132 tRNA genes, 69 ncRNA genes, and 9 CRISPRs structures. Additionally, 4643, 3171, 4086, 3232, 2034 and 238 genes were annotated in the NR, Swiss Prot, eggNOG, GO, KEGG and CAZy databases, respectively. Twelve biosynthetic gene clusters(BGCs) for secondary metabolites were identified using antiSMASH, three of which showed high homology with fusaricidin B, tridecaptin, and thermoactinoamide A. Comparative genomic analysis with P. polymyxa J and SC2, which exhibit biocontrol function, revealed that strain Pp146 possesses a smaller genome size, higher GC content, and contains 57 specific gene clusters. Plate confrontation assays and fermentation filtrate inhibition tests confirmed that Pp146 achieved inhibition rates of over50.00% against three pathogenic fungi causing diseases in Rehmannia glutinosa, Achyranthes bidentata, and Dioscorea opposita. Additionally, its fermentation culture filtrate exhibited a maximum spore germination inhibition rate of 43.38%. Pot experiments demonstrated that pre-inoculation with Pp146 culture filtrate twice resulted in a control efficacy up to 68.94% against three Fusarium diseases. Furthermore, single application with Pp146 significantly enhanced the accumulation of belowground biomass in host plants, with fresh root weight increases ranging from 34.24% to 164.53%. In conclusion, strain Pp146 exhibits both broad-spectrum antifungal and plant growth-promoting activities, demonstrating great potential for development in ecological cultivation of medicinal plants.

Key words: Paenibacillus polymyxa, whole-genome sequencing, growth-promoting, disease control, medicinal plant

CLC Number:

S476

LI Xuemeng, LI Shaojian, YANG Jin, SONG Luyang, GUO Zhichen, LIU Qi, GAO Suxia, QIN Yanhong, LU Shuhao, SHEN Danyu, WANG Fei. Genome Sequencing of Paenibacillus polymyxa Pp146 and Evaluation of Its Growth-Promoting and Disease Control Effects on Medicinal Plants[J]. Chinese Journal of Biological Control, 2026, 42(3): 621-636.

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