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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (5): 1296-1307.DOI: 10.16409/j.cnki.2095-039x.2022.06.008

• RESEARCH REPORTS • Previous Articles     Next Articles

Screening of Disease-control and Growth-Promoting Bacteria and Their Effecs on Lanzhou Lily

LI Xueping1, ZHANG Yixin2, LI Jianjun1, XU Shiyang2, QI Yonghong1, JING Zhuoqiong1, GUO Zhijie1, LI Minquan1   

  1. 1. Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China;
    2. College of Prataculture, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2022-03-10 Published:2022-10-20

Abstract: A serious of soil problems were caused by lily continuous cropping in Lanzhou,from which the soil-borne diseases such as Fusarium wilt were prominent.There were many functional strains in the rhizosphere of healthy lily,and 140 strains with plant disease-control and growth-promoting function were screened in this study,so as to provide safe and effective biocontrol methods against soil-borne diseases.Among all the strains,81 had antagonistic ability,102 with organophosphorus phosphate solubilization capacity,82 with inorganic phosphate solubilization capacity,118 with nitrogen-fixing capacity and 32 with potassium solubilization capacity.Then their abilities were quantified and several strains with excellent abilities were selected for random combination fermentation,and the capacities of the combinations were determined,respectively.Topsis comprehensive analysis showed BP5 was the best of the 5 excellent selected combinations (BP1~BP5),which contained 4 strains of bacteria.JK21 was identified as Brevibacterium frigoritolerans,JK22 was Bacillus cereus,both N11 and M40 were Bacillus subtilis.BP5 was used as the formula to make the microbial inoculum and demonstrated excellent capacities,with the disease-control effect above 68%,both the yield and the quality increased.The fresh weight of the bulb increased by 40 g compared with control group (CK),and the soluble sugar,reducing sugar,crude protein,crude fat,Vc and flavone increased,while crude ash and crude fiber decreased.Besides,the soil quality,such as available potassium,alkali-hydrolyzed nitrogen,soil urease,sucrase and alkaline phosphatase,increased significantly,and the nitrogen-fixing genes,aerobic and anaerobic autogenous nitrogen-fixing bacteria increased significantly in rhizosphere soil.The above results indicated that the BP5 combination could solve the continuous cultivation obstacle of Lanzhou lily and improve soil quality.

Key words: Lanzhou lily, Fusarium wilt, disease-control and growth-promoting, Bacillus subtilis

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