Screening of Disease-control and Growth-Promoting Bacteria and Their Effecs on Lanzhou Lily

doi:10.16409/j.cnki.2095-039x.2022.06.008

Abstract

Abstract: A serious of soil problems were caused by lily continuous cropping in Lanzhou,from which the soil-borne diseases such as Fusarium wilt were prominent.There were many functional strains in the rhizosphere of healthy lily,and 140 strains with plant disease-control and growth-promoting function were screened in this study,so as to provide safe and effective biocontrol methods against soil-borne diseases.Among all the strains,81 had antagonistic ability,102 with organophosphorus phosphate solubilization capacity,82 with inorganic phosphate solubilization capacity,118 with nitrogen-fixing capacity and 32 with potassium solubilization capacity.Then their abilities were quantified and several strains with excellent abilities were selected for random combination fermentation,and the capacities of the combinations were determined,respectively.Topsis comprehensive analysis showed BP5 was the best of the 5 excellent selected combinations (BP1~BP5),which contained 4 strains of bacteria.JK21 was identified as Brevibacterium frigoritolerans,JK22 was Bacillus cereus,both N11 and M40 were Bacillus subtilis.BP5 was used as the formula to make the microbial inoculum and demonstrated excellent capacities,with the disease-control effect above 68%,both the yield and the quality increased.The fresh weight of the bulb increased by 40 g compared with control group (CK),and the soluble sugar,reducing sugar,crude protein,crude fat,Vc and flavone increased,while crude ash and crude fiber decreased.Besides,the soil quality,such as available potassium,alkali-hydrolyzed nitrogen,soil urease,sucrase and alkaline phosphatase,increased significantly,and the nitrogen-fixing genes,aerobic and anaerobic autogenous nitrogen-fixing bacteria increased significantly in rhizosphere soil.The above results indicated that the BP5 combination could solve the continuous cultivation obstacle of Lanzhou lily and improve soil quality.

Key words: Lanzhou lily, Fusarium wilt, disease-control and growth-promoting, Bacillus subtilis

CLC Number:

S476

LI Xueping, ZHANG Yixin, LI Jianjun, XU Shiyang, QI Yonghong, JING Zhuoqiong, GUO Zhijie, LI Minquan. Screening of Disease-control and Growth-Promoting Bacteria and Their Effecs on Lanzhou Lily[J]. Chinese Journal of Biological Control, 2022, 38(5): 1296-1307.

References

[1] 李瑞琴,于安芬,白滨,等.食用百合-土壤体系中镉、铅和汞的潜在生态和健康风险[J].食品科学, 2016, 37(5):186-197.
[2] Hou R, Jin C, Yue C, et al. Modification of lily polysaccharide by selenylation and the immuneenhancing activity[J]. Carbohydr Polym, 2016, 142:73-81.
[3] 梁旭东,毕春辉,李雪.国家地理标志产品-兰州百合种植技术规程[J].蔬菜, 2019, 12:60-62.
[4] 陈君良.兰州百合根系分泌物自毒作用的研究及化感物质的GC-MS分析[D].兰州:甘肃农业大学, 2016:15-26.
[5] Dou F, Wright A L, Mylavarapu R S, et al. Soil enzyme activities and organic matter composition affected by 26 years of continuous cropping[J]. Pedosphere, 2016, 26:618-625.
[6] Yu M, Yu J W, Gao P G, et al. Agrochemical characteristics of soil for continuous cropping lily[J]. Chinese Journal of Soil Science, 2004, 35:377-379.
[7] Shang Q H, Zhao X, Li YY, et al. First Report of Fusarium tricinctum causing stem and root rot on Lanzhou lily (Lilium davidii var. unicolor) in China[J]. Plant Disease, 2014, 98:999-1000.
[8] Schloter M, Dilly O, Munch J C. Indicators for evaluating soil quality[J]. Agriculture Ecocystems and Eviroment, 2003, 98:255-262.
[9] Liu X B, Stephen J H. Fifteen years of research examining cultivation of continuous soybean in northeast China:a review[J]. Field Crops Research, 2002, 79:1-7.
[10] 白滨,杨花莲,何苏琴,等.兰州百合叶枯病病原菌形态特征及生物学特性研究[J].中国蔬菜, 2013, 16:78-84.
[11] Dutta S, Podile A R. Plant Growth Promoting Rhizobacteria (PGPR):The bugs to debug the root zone[J]. Critical Reviews Microbiology, 2010, 36:232-244.
[12] Swarnalakshmi K, Yadav V, Tyagi D, et al. Significance of plant growth promoting rhizobacteria in grain legumes:Growth promotion and crop production[J]. Plants, 2020, 9(11):1596-1596.
[13] Kalam S, Basu A, Podile A R. Functional and molecular characterization of plant growth promoting Bacillus isolates from tomato rhizosphere[J]. Heliyon, 2020, 6:e04734.
[14] Khoshru B, Mitra D, Khoshmanzar E, et al. Current scenario and future prospects of plant growth-promoting rhizobacteria:An economic valuable resource for the agriculture revival under stressful conditions[J]. Journal of Plant Nutrition, 2020, 43:3062-3092.
[15] 温宏伟,杨斌,王东胜.植物根际促生菌促进小麦生长及提高其抗旱性的研究进展[J].核农学报, 2021, 35(9):2194-2203.
[16] 陈腊,米国华,李可可,等.多功能植物根际促生菌对东北黑土区玉米的促生效果[J].应用生态学报, 2020, 31(8):2759-2766.
[17] 许世洋,李敏权,刘梅金,等.青稞根腐病防病促生细菌的筛选及其菌剂防效[J].微生物学通报, 2022, 49(7):3575-2586.
[18] 许世洋,范雨轩,汪学苗,等.辣椒镰孢根腐病防病促生细菌的筛选及其效应[J].微生物学报, 2022, 62(7):2735-2750.
[19] Vaikuntapu P R, Dutta S, Samudrala R B, et al. Preferential promotion of Lycopersicon esculentum(tomato) growth by plant growth promoting bacteria associated with tomato[J]. Indian Journal of Microbiology, 2014, 54:403-412.
[20] 曲春鹤,何付丽,刘培福,等.辣椒根腐病拮抗细菌的筛选、鉴定及其抑菌促生作用[J].东北农业大学学报, 2012, 43(7):89-94.
[21] 杨茉,高婷,李滟璟,等.辣椒根际促生菌的分离筛选及抗病促生特性研究[J].生物技术通报, 2020, 36(5):104-109.
[22] 鲍士旦.土壤农化分析[M].第三版.北京:中国农业出版社, 2000, 79-86.
[23] 李雪萍,李敏权,孟宪刚,等.浆水中降胆固醇乳酸菌的筛选及其功能特性[J].微生物学报, 2015, 55(8):1001-1009.
[24] 中华人民共和国农业部.蔬菜及其制品中可溶性糖的测定铜还原碘量法:NY/T 1278-2007[S].北京:中国标准出版社, 2007.
[25] 中华人民共和国卫生部,中国国家标准化管理委员会.植物类食品中粗纤维的测定:GB/T 5009.10-2003[S].北京:中国标准出版社, 2003.
[26] 中华人民共和国国家卫生和计划生育委员会.食品中灰分的测定:GB 5009.4-2016[S].北京:中国标准出版社, 2016.
[27] 中华人民共和国国家卫生和计划生育委员会.食品中蛋白质的测定:GB 5009.5-2010[S].北京:中国标准出版社, 2010.
[28] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.食品中粗脂肪的测定:GB/T 14772-2008[S].北京:中国标准出版社, 2008.
[29] 中华人民共和国国家卫生和计划生育委员会.食品中还原糖的测定:GB 5009.7-2016[S].北京:中国标准出版社, 2016.
[30] 邹荣.不同水果中维生素C含量的测定及其影响因素研究[J].食品安全导刊. 2021, 21:190-192.
[31] 姜喜,唐章虎,吴翠云,等. 3种梨果实发育过程中酚类物质及其抗氧化能力分析[J].食品科学, 2021, 42(23):99-105.
[32] 张兆永,李菊英,祖皮艳木·买买提,等.艾比湖流域小尺度农田土壤养分的空间分布和盐渍化风险评价[J].生态学报, 2017, 37(3):819-828.
[33] 李雪萍,漆永红,郭炜,等.青稞根腐病对根际土壤微生物及酶活性的影响[J].生态学报, 2017, 37(17):5640-5649.
[34] 吴小虎.氟磺胺草醚对土壤微生物多样性的影响[D].北京:中国农业科学院博士学位论文, 2014.
[35] 姚拓,龙瑞军,师尚礼,等.高寒草地不同扰动生境土壤微生物氮素生理群数量特征研究[J].土壤学报, 2007, 44(1):122-129.
[36] Mahdi I. Potential application of selected phosphate solubilizing bacteria isolated from Chenopodium quinoa Willd rhizosphere in early plant growth promotion[J]. Enzyme Engineering, 2021, 10(5):3.
[37] 撖冬荣,侯栋,姚拓,等.莴笋根部促生菌筛选与促生特性测定[J].干旱地区农业研究, 2020, 38(3):127-133.
[38] 陈建爱,贾梦莹,陈为京. PDA中水含量对固体培养黄绿木霉T1010分生孢子生长发育的影响[J].中国农学通报, 2020, 36(15):106-114.
[39] Maris S, Vizma Na, Galina M, Zaiga P. Influence of Trichoderma asperellum and Bacillus subtilis as biocontrol and plant growth promoting agents on soil microbiota[J]. Annals of Microbiology, 2021, 71:34.
[40] Yang W. Components of rhizospheric bacterial communities of barley and their potential for plant growth promotion and biocontrol of Fusarium wilt of watermelon[J]. Brazilian Journal of Microbiology, 2019, 50:749-757.
[41] Monu J, Gupta V K, Kousik M. Brevibacterium frigoritolerans as a novel organism for the bioremediation of phorate[J]. Bull Environ Contam Toxicol, 2015, 95:680-686.
[42] Alejandra C L, Lizeth G V, Miriam B G, et al. Effect of Metabolites Produced by Bacillus atrophaeus and Brevibacterium frigoritolerans strains on postharvest biocontrol of Alternaria alternata in tomato (Solanum lycopersicum L.)[J]. Biocontrol Science, 2021, 26(2):67-74.
[43] 王明湖,连瑛,李雅颖,等.不同微生物菌剂对草莓炭疽病防控和土壤微生物群落结构的影响[J].浙江农业科学, 2022, 63(4):1-4.
[44] Ji C, Huang J, Tian Y, et al. Feasibility Study on the application of microbial agent modified water-jet loom sludge for the restoration of degraded soil in mining areas[J]. International Journal of Environmental Research and Public Health, 2021, 18(13):6797-6797.
[45] 温丹,王晓,孙凯宁,等.不同形态微生物菌剂对不结球白菜生长和品质的影响[J].应用生态学报, 2021, 32(5):1777-1782.
[46] Deng L, Wang T, Luo W, et al. Effects of a compound microbial agent and plants on soil properties, enzyme activities, and bacterial composition of Pisha sandstone[J]. Environmental Science and Pollution Research, 2021, 28(38):1-12.
[47] Jin X, Ai W, Zhang Y, Dong W. Application of functional microbial agent in aerobic composting of wheat straw for waste recycling[J]. Life Sciences in Space Research, 2022, 33:13-20.
[48] Wang C, Wu M, Peng C, et al. Bacterial dynamics and functions driven by a novel microbial agent to promote kitchen waste composting and reduce environmental burden[J]. Journal of Cleaner Production, 2022, 337:1-10.
[49] 王国丽,张晓丽,张晓霞,等.施用功能微生物菌剂对重度盐碱地向日葵生长及土壤微生物的影响[J].中国土壤与肥料, 2021,(5):133-139.
[50] 王丽丽,朱诗君,狄蕊,等.微生物菌肥菌剂对番茄生长发育和产量品质的影响[J].土壤与作物, 2022, 11(1):88-95.