Insecticidal Activity of Four Different Bt Toxins against Six Important Lepidopteran Pests

doi:10.16409/j.cnki.2095-039x.2017.06.009

Abstract

Abstract: Selection of Bt toxin with high efficiency against target pests and low harm to non-target creatures is very important for the development of novel Bt bio-insecticide or insecticidal gene in the future. We tested the insecticidal efficiency of 4 different Bt toxins (Cry2Ab4, Cry2Ah1, Cry1Ca7 and Vip3Aa11) against 6 species of lepidopterans pests, including Helicverpa armigera, Spodoptera exigua, Spodoptera litura, Mythimna separate, Ostrinia furnacalis and Chilo suppressalis. The results showed that Cry2Ab4 had wide insecticidal spectrum, it had high insecticidal efficiency against all the six species of lepidopteran pests; especially, it had obvious toxicity effect on H. armigera, S. exigua, S. litura and O. furnacalis. Cry2Ah1 had good lethal effect on H. armigera, S. exigua, S. litura and had certain efficiency against O. furnacalis and C. suppressalis; meanwhile it had significant weight inhibition effect on M. separate rather than lethal effect. Cry1Ca7 had obvious control efficiency against S. exigua and S. litura,and also good effects on H. armigera and C. suppressalis. Cry1Ca7 had lower insecticidal efficiency against O. furnacalis and no activity against M. separate. Vip3Aa11 had good efficiency against S. exigua and S. litura, and adequate effects on O. furnacalis, H. armigera and C. suppressalis, while had no activity against M. separate.

Key words: Bt, lepidopteran pests, insecticidal efficiency, pest control

CLC Number:

S476.12

LIU Chen, CHEN Lin, WANG Bingjie, ZHAO Man, LIANG Gemei, GUO Yuyuan. Insecticidal Activity of Four Different Bt Toxins against Six Important Lepidopteran Pests[J]. journal1, 2017, 33(6): 774-779.

References

[1] 喻子牛, 孙明, 刘子铎, 等. 苏云金芽孢杆菌的分类及生物活性蛋白基因[J]. 中国生物防治, 1996, 12(2):85-89.
[2] Liliana P L, Mario S, Alejandra B. Bacillus thuringiensis insecticidal three-domain Cry toxins:mode of action, insect resistance and consequences for crop protection[J]. FEMS Microbiology Reviews, 2013, 37:3-22.
[3] Wei J Z, Liang G M, Wang B J, et al. Activation of Bt protoxin Cry1Ac in resistant and susceptible cotton bollworm[J]. PLoS ONE, 2016, 11(6):e0156560
[4] Tabashnik B E, Gassmann A J, Crowder D W, et al. Insect resistance to Bt crops:evidence versus theory[J]. Nature Biotechnology, 2008, 26(2):199-202.
[5] Shelton A M, Robertson J L, Tang J D, et al. Resistance of diamondback moth (Lepidoptera:Plutellidea) to Bacillus thuringensis subspecies in the field[J]. Journal of Economic Entomology, 1993, 86(3):697-705.
[6] Qiao F, Huang J, Rozelle S, et al. Natural refuge crops, buildup of resistance, and zero-refuge strategy for Bt cotton in China[J]. China Life Sciences, 2010, 53(10):1227-1238.
[7] 郭芳, 梁革梅, 曹广春, 等. 昆虫对Bt抗性的适合度代价及其与抗性治理策略的关系[J]. 环境昆虫学报, 2009, 31(2):162-167.
[8] Tabashnik B E, Gassmann A J, Crowder D W, et al. Insect resistance to Bt crops:evidence versus theory[J]. Nature Biotechnology, 2008, 26(2):199-202.
[9] Macintosh S C. Managing the risk of insect resistance to transgenic insect control traits:practical approaches in local environments[J]. Pest Management Science, 2010, 66(1):100-106.
[10] Liu F, Xu Z, Chang J, et al. Resistance allele frequency to Bt cotton in field populations of Helicoverpa armigera (Lepidoptera:Noctuidae) in China[J]. Journal of Economic Entomology, 2008, 101(3):933-943.
[11] Zhang H, Yin W, Zhao J, et al. Early warning of cotton bollworm resistance associated with intensive planting of Bt cotton in China[J]. PLoS ONE, 2011, 6(8):e22874.
[12] Li B, Xu Y Y, C Han C, et al. Chilo suppressalis and Sesamia inferens display different susceptibility responses to Cry1A insecticidal proteins[J]. Pest Management Science, 2015, 71(10):1433-1440
[13] 谢宝玉, 汪恩国. 水稻二化螟再猖獗发生原因及综合治理[J]. 植物保护, 2000, 26(6):46-47.
[14] 梁革梅, 谭维嘉, 郭予元. 人工饲养棉铃虫技术的改进[J]. 植物保护, 1999, 25(2):15-17.
[15] 路子云, 冉红凡, 刘文旭, 等. 粘虫室内饲养方法的改进[J]. 河北农业科学, 2014, 18(6):57-61.
[16] 宋彦英, 周大荣, 何康来. 亚洲玉米螟无琼脂半人工饲料的研究与应用[J]. 植物保护, 1999, 26(4):324-328.
[17] 李波, 韩兰芝, 彭于发. 二化螟人工饲养技术[J]. 应用昆虫学报, 2015, 52(2):498-503.
[18] 张志涛, 刘金生, 许强, 等. Bradford法测定牛奶中蛋白质含量[J]. 食品与机械, 2011, 27(5):128-130.
[19] 尹凤翔, 孙凯, 束长龙, 等. Cry1Ba3蛋白对亚洲玉米螟杀虫活性测定及其与BBMVs的结合分析[J]. 植物保护, 2014, 40(2):22-27.
[20] Zhou Z S, Yang S J, Shu C L, et al. Comparison and optimization of the method for Cry1Ac protoxin preparation in HD73 strain[J]. Journal of Integrative Agriculture, 2015, 14(8):1598-1603.
[21] 贾春生. 利用SPSS软件计算杀虫剂的LC₅₀[J]. 昆虫知识, 2006, 43(3):414-417.
[22] 彭琦, 周子珊, 张杰. 苏云金芽胞杆菌杀虫晶体蛋白研究进展[J]. 中国生物防治学报, 2015, 31(5):712-722.
[23] Li X Q, Tan A, Voegtline M, et al. Expression of Cry5B protein from Bacillus thuringiensis in plant roots confers resistance to root-knot nematode[J]. Biological Control, 2008, 47(1):97-102.
[24] Chakroun M, Bel Y, Caccia S, et al. Susceptibility of Spodoptera frugiperda and S. exigua to Bacillus thuringiensis Vip3Aa11 insecticidal protein[J]. Journal of Invertebrate Pathology, 2012, 110(3):334-339.
[25] Whalon M E, Wingerd B A. Bt:mode of action and use[J]. Archives of Insect Biochemistry and Physiology, 2003, 54(4):200-211.
[26] Welch K L, Unnithan G C, Degain B A, et al. Cross-resistance to toxins used in pyramided Bt crops and resistance to Bt sprays in Helicoverpa zea[J]. Journal of Invertebrate Pathology, 2015, 132:149-156.
[27] 谭声江, 陈晓峰, 李典谟. 棉铃虫对转Bt基因棉的抗性及其治理策略研究进展[J]. 昆虫学报, 2002, 45(1):138-144.
[28] 陈建武, 余健秀, 胡晓晖, 等. 苏云金芽孢杆菌营养期杀虫蛋白的研究[J]. 中国生物工程杂志, 2002, 22(3):33-36.