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journal1 ›› 2018, Vol. 34 ›› Issue (4): 546-552.DOI: 10.16409/j.cnki.2095-039x.2018.04.008

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Screening of Insecticidal Activity Protein against Holotrichia parallela Adults from Bacillus thuringiensis

HUANG Shizhi1,2, SHU Changlong2, LIU Chunqin3, DING Xuezhi1, ZHANG Jie2   

  1. 1. State Key Laboratory of Developmental Biology of Freshwater Fishes/Hunan Provincial Key Laboratory of Microbial Molecular Biology/College of Life Science, Hunan Normal University, Changsha 410081, China;
    2. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    3. Institute of Plant Protection, Cangzhou Academy of Agricultural and Forestry Sciences, Cangzhou 061001, China
  • Received:2018-04-19 Online:2018-08-08 Published:2018-07-30

Abstract: Holotrichia parallela is an important agricultural pests, damaging several crops at both the adult and larval stages. To screen Bacillus thuringiensis protein of high insecticidal activity a bioassay method of the Bt protein against H. parallela adults was established by feeding on the adults with Ulmus pumila L leaves soaked in Bt proteins. Six Cry8 full-length crystal proteins and trypsin-digested proteins were prepared and used in the insecticidal activity bioassay. Cry8Ga1 protoxin and digested protein showed high insecticidal activity in the primary test, with 7-day corrected mortality rates of 100% and 55%, respectively, at the concentration of 1.0 mg/mL. Further, the median lethal concentration (LC50) values were determined to be 0.387 mg/mL (95% CL 0.108-0.746) for Cry8Ga1 protoxin and 0.702 mg/mL (95% CL 0.150-2.664) for digested Cry8Ga1 protein. When fed with Cry8Ga1 protoxin and digested protein, H. parallela adults exhibited obvious pathological changes at the organelles in midgut cells. The Bt protein showing high insecticidal activity against H. parallela adults is a new approach for the biological control of Scarabaeoideas.

Key words: Bacillus thuringiensis, Cry8Ga1 protein, Holotrichia parallela adults, insecticidal activity

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