Isolation and Identification of Biocontrol Bacterium and Its Control Effect against Meloidogyne graminicola

doi:10.16409/j.cnki.2095-039x.2018.04.016

Abstract

Abstract: In order to obtain biocontrol bacteria which can efficiently control Meloidogyne graminicola, soil bacteria were isolated and screened in vitro for their effects on mortality of Ditylenchus destructor and M. graminicola. From primary screening against D. destructor and second screening against second-stage juveniles of M. graminicola, 8 soil bacteria secreting high nematocidal metabolites were isolated. Among them, strain D45 showed the highest activity. After 24 h and 48 h incubation, the supernatant of strain D45 culture diluted five times resulted in a mortality of 69.8% and 72.1% to D. destructor, 60.9% and 70.4% to M. graminicola, respectively. Pot and field experiments indicated that soil drenching with fermentation broth of strain D45 prior to nematode inoculation greatly reduced the numbers of galls. The nematicidal substances of strain D45 were sensitive to protease,while insensitive to acid and heating. Based on physiological and biochemical characters, 16S rRNA gene sequence analysis, D45 was identified to be Bacillus megaterium. This study demonstrates that B. megaterium D45 has good application potential in controlling of M. graminicola.

Key words: Bacillus megaterium, biocontrol, Meloidogyne graminicola, bioactive metabolites

CLC Number:

S476

YAO Simin, DING Zhong, ZHOU Jianyu, YUAN Tao, HUANG Yongchun, YE Shan. Isolation and Identification of Biocontrol Bacterium and Its Control Effect against Meloidogyne graminicola[J]. journal1, 2018, 34(4): 606-615.

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