Biocontrol Effect and Mechanism of Four Strains of Antagonistic Bacteria against Cotton Verticillium Wilt

doi:10.16409/j.cnki.2095-039x.2018.06.011

Abstract

Abstract: Cotton Verticillium wilt has seriously restricted the high and stable yields of cotton in Xinjiang. Antagonistic bacteria have great potential for biological control of cotton Verticillium wilt. The objective of this study is to explore the biocontrol effect and mechanism of four strains of antagonistic bacteria against cotton Verticillium wilt and to provide theoretical basis for the biological control of cotton Verticillium wilt. The effect of four strains of antagonistic bacteria on the conidial concentration, mycelium and spore morphology, membrane permeability and toxin production ability of Verticillium dahliae were investigated with co-culture method. The biocontrol efficacy of four strains of antagonistic bacteria against cotton Verticillium wilt was detected by culture filtrate inoculation method in pot experiments. The activities of defense enzymes in cotton plants were determined by guaiacol method and nitroblue tetrazolium photoreduction method. The results of co-culture showed that four strains of antagonistic bacteria could reduce the conidial concentration of Verticillium dahliae, destroy cell morphology, cause cell membrane rupture and decrease toxic protein concentration. Inoculation with four strains of antagonistic bacteria could induce the accumulation of defense enzymes such as POD and SOD enzymes in cotton and induce the disease resistance of cotton, and the maximum biocontrol efficacy treated with strains SHZ-24, SHT-15, SMT-24 and BHZ-29 were 73.82%, 80.48%, 84.91% and 84.18%, respectively. The four antagonistic bacteria could improve the cotton resistance to V. dahliae by inhibiting the pathogen growth and inducing plant defense response, which had a good biocontrol effect on cotton Verticillium wilt.

Key words: antagonistic bacteria, cotton Verticillium wilt, biocontrol effect, mechanism, induced resistance

CLC Number:

S476

ZHANG Tao, Li Xueyan, YANG Hongmei, CHU Min, GAO Yan, ZENG Jun, HUO Xiangdong, ZHANG Tao, LIN Qing, MAHEMUTI Outikuer, LI Yuguo, LOU Kai, SHI Yingwu. Biocontrol Effect and Mechanism of Four Strains of Antagonistic Bacteria against Cotton Verticillium Wilt[J]. journal1, 2018, 34(6): 882-889.

References

[1] 刘海洋, 努尔孜亚, 毕海燕, 等. 新疆棉花黄萎病抗性鉴定与评价[J]. 新疆农业科学, 2012, 49(5):873-878.
[2] Chen B, Liu Z. Study on cotton Verticillium wilt[J]. Plant Diseases and Pests, 2012, 3(2):15-20, 23.
[3] 马存, 简桂良, 孙文姬. 我国棉花抗黄萎病育种现状、问题及对策[J]. 中国农业科学, 1997, 32(2):58-64.
[4] 曾红, 杨生强. 棉花黄萎病菌拮抗细菌TUBP1的分离鉴定及其防病作用[J]. 棉花学报, 2014, 26(5):445-451.
[5] Yang P, Sun Z X, Liu S Y, et al. Combining antagonistic endophytic bacteria in different growth stages of cotton for control of Verticillium wilt[J]. Crop Protection, 2013, 47(5):17-23.
[6] Berg G, Fritze A, Roskot N, et al. Evaluation of potential biocontrol rhizobacteria from different host plants of Verticillium dahliae Kleb.[J]. Journal of Applied Microbiology, 2001, 91(6):963-971.
[7] Berg G, Kurze S, Buchner A, et al. Successful strategy for the selection of new strawberry-associated rhizobacteria antagonistic to Verticillium wilt[J]. Canadian Journal of Microbiology, 2000, 46(12):1128-1137.
[8] Zhu H Q, Feng Z L, Li Z F, et al. Characterization of two fungal isolates from cotton and evaluation of their potential for biocontrol of Verticillium wilt of cotton[J]. Journal of Phytopathology, 2013, 161(2):70-77.
[9] Zhang Y, Xue Q Y, Xu L L, et al. A screening strategy of fungal biocontrol agents towards Verticillium wilt of cotton[J]. Biological Control, 2011, 56(3):209-216.
[10] Xue L, Xue Q H, Chen Q. Isolation and evaluation of rhizosphere actinomycetes with potential application for biocontrol of Verticillium wilt of cotton[J]. Crop Protection, 2013, 43:231-240.
[11] Han Q, Wu F, Wang X, et al. The bacterial lipopeptide iturins induce Verticillium dahliae cell death by affecting fungal signaling pathways and mediate plant defense responses involved in pathogen-associated molecular pattern-triggered immunity[J]. Environmental Microbiology, 2015, 17(4):1166-1188.
[12] Li C H, Shi L, Han Q, et al. Biocontrol of Verticillium wilt and colonization of cotton plants by an endophytic bacterial isolate[J]. Journal of Applied Microbiology. 2012, 113(3):641-652.
[13] Raza W, Yang W, Shen Q R. Paenibacillus polymyxa:antibiotics, hydrolytic enzymes and hazard assessment[J]. Journal of Plant Pathology, 2008, 90(3):419-430.
[14] Niu B, Vater J, Rueckert C, et al. Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1[J]. BioMed Central Microbiology, 2013, 13:137-149.
[15] Niemann H, Lösekann T, de Beer D, et al. Novel microbial communities of the Haakon Mosby mud volcano and their role as a methane sink[J]. Nature. 2006, 443(7113):854-858.
[16] Sedmak J J, Grossberg S E. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250[J]. Analytical Biochemistry, 1977, 79(1):544-552.
[17] 沈其益. 棉花病害基础研究与防治[M]. 北京:科学出版社, 1992.
[18] 李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社, 2000.
[19] 傅正擎, 夏正俊, 吴蔼民, 等. 内生菌防治棉花黄萎病机理[J]. 江苏农业学报, 1999, 15(4):20-24.
[20] Deketelaere S, Tyvaert L, França S C, et al. Desirable traits of a good biocontrol agent against Verticillium wilt[J]. Frontiers in Microbiology, 2017, 8:1186-1192.
[21] Angelopoulou D J, Naska E J, Paplomatas E J, et al. Biological control agents (BCAs) of Verticillium wilt:plant protection, triggering of host defense mechanisms and rhizosphere populations of BCAs are influenced by application rates and delivery method[J]. Plant Pathology, 2014, 63(5):1062-1069.
[22] Berg G, Ballin G. Bacterial antagonists to Verticillium dahliae Kleb.[J]. Journal of Phytopathology, 2010, 141(1):99-110.
[23] 田绍仁, 聂太礼, 王梦亮, 等. 拮抗细菌C-02防治棉花黄萎病的机理研究[J]. 棉花学报, 2012, 24(5):420-426.
[24] Krassilnikov N A, Khodjibayeva C M, Mirchink T G. Properties of toxins of Verticillium dahliae, the causative agent of cotton wilt disease[J]. The Journal of General and Applied Microbiology, 1969, 15(1):1-9.
[25] 章元寿, 王建新, 方中达. 大丽轮枝菌毒素致萎活性成分的研究[J]. 菌物学报, 1990, 9(1):69-72.
[26] 吕金殿, 甘莉, 阎龙飞. 棉花黄萎病菌毒素的纯化与特性研究[J]. 植物病理学报, 1991, 21(2):51-55.
[27] 范尉尉, 马春红, 董文琦, 等. 棉花根部损伤程度对感染黄萎病菌后体内抗性酶的影响[J]. 中国农业科学, 2006, 39(12):2483-2490.
[28] 张芸, 冯自力, 冯鸿杰, 等. 内生球毛壳属真菌CEF-082对棉花黄萎病的控制作用[J]. 植物病理学报, 2016, 46(5):697-706.
[29] 周京龙, 冯自力, 冯鸿杰, 等. 棉花内生蜡状芽孢杆菌YUPP-10对棉花黄萎病的防治作用及机制[J]. 中国农业科学, 2017, 50(14):2717-2727.
[30] 李欣欣, 王秀红, 史向远, 等. 堆肥提取液诱导草莓对黄萎病抗性及抑菌机理研究[J]. 中国生物防治学报, 2018, 34(2):294-302.
[31] 马春红, 范尉尉, 董文琦, 等. 黄萎病菌毒素粗提液对棉花抗性酶的诱导[J]. 中国农业科学, 2008, 41(12):4314-4320.