Welcome to Chinese Journal of Biological Control,Today is

journal1 ›› 2018, Vol. 34 ›› Issue (6): 882-889.DOI: 10.16409/j.cnki.2095-039x.2018.06.011

Previous Articles     Next Articles

Biocontrol Effect and Mechanism of Four Strains of Antagonistic Bacteria against Cotton Verticillium Wilt

ZHANG Tao1,2, Li Xueyan1,2, YANG Hongmei1, CHU Min1, GAO Yan1, ZENG Jun1, HUO Xiangdong1, ZHANG Tao1, LIN Qing1, MAHEMUTI Outikuer1, LI Yuguo1, LOU Kai1, SHI Yingwu1   

  1. 1. College of Life Science and Technology, Xinjiang University, Urumqi 830046, China;
    2. Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
  • Received:2018-04-23 Online:2018-12-08 Published:2018-12-08

Abstract: Cotton Verticillium wilt has seriously restricted the high and stable yields of cotton in Xinjiang. Antagonistic bacteria have great potential for biological control of cotton Verticillium wilt. The objective of this study is to explore the biocontrol effect and mechanism of four strains of antagonistic bacteria against cotton Verticillium wilt and to provide theoretical basis for the biological control of cotton Verticillium wilt. The effect of four strains of antagonistic bacteria on the conidial concentration, mycelium and spore morphology, membrane permeability and toxin production ability of Verticillium dahliae were investigated with co-culture method. The biocontrol efficacy of four strains of antagonistic bacteria against cotton Verticillium wilt was detected by culture filtrate inoculation method in pot experiments. The activities of defense enzymes in cotton plants were determined by guaiacol method and nitroblue tetrazolium photoreduction method. The results of co-culture showed that four strains of antagonistic bacteria could reduce the conidial concentration of Verticillium dahliae, destroy cell morphology, cause cell membrane rupture and decrease toxic protein concentration. Inoculation with four strains of antagonistic bacteria could induce the accumulation of defense enzymes such as POD and SOD enzymes in cotton and induce the disease resistance of cotton, and the maximum biocontrol efficacy treated with strains SHZ-24, SHT-15, SMT-24 and BHZ-29 were 73.82%, 80.48%, 84.91% and 84.18%, respectively. The four antagonistic bacteria could improve the cotton resistance to V. dahliae by inhibiting the pathogen growth and inducing plant defense response, which had a good biocontrol effect on cotton Verticillium wilt.

Key words: antagonistic bacteria, cotton Verticillium wilt, biocontrol effect, mechanism, induced resistance

CLC Number: