Screening and Efficiency Evaluation of Pseudomonas Strain in the Control of Magnaporthe oryzae

doi:10.16409/j.cnki.2095-039x.2020.01.010

Abstract

Abstract: In order to screen Pseudomonas strains for biological control of Magnaporthe oryzae, 291 bacterial isolates were obtained from rice soil in Ningxia Hui Autonomous Region. Only strain S149 was found strongly antagonistic to M. oryzae in dual culture technique. The antagonistic rate of hyphal growth of M. oryzae P131 inhibited by strain S149 after 5 d was 83.33% in vitro. In addition, the antagonistic rates of its fermentation broth and sterile supernatant were 67.39% and 56.52%, respectively. 16S rDNA sequence analysis indicated that strain S149 had the highest homology with P. fluorescens. Combining with its morphological characteristic, the strain S149 was identified as P. fluorescens. Strain S149 was also found measurable antagonistic to 10 plant pathogens, such as Fusarium spp., Botrytis cinerea, Phytophthora parasitica var. nicotianae. P. fluorescens S149 could strongly inhibit the conidial germination and appressorial formation of M. oryzae P131. The inhibitory efficacies of the fermented liquid were 89.71% and 94.36%, respectively, and those of the sterile supernatant were 81.77% and 86.95%, respectively. The preventive efficacies of P. fluorescens strain S149 for the control of rice blast was 79.98% under greenhouse condition. Field trials showed that the strain could promote rice growth, increase the germination rate by 7.15%, promote rice root length by 10.36%, and enhance rice plant height by 10.33%. Preventive efficacies of P. fluorescens S149 was 73.32% against leaf blast, and 71.55% and 70.21% against neck blast, respectively. Moreover, there was no significant difference between the preventive efficacy of strain S149 and 75% tricyclazole wettable powder, and there was significant difference between the preventive efficacy of strain S149 and Lvdikang. The results indicated that P. fluorescens S149 could not only prevent rice blast, but also promote the rice growth, and have potential to be applied in various fungal diseases control.

Key words: rice blast, Pseudomonas, screen, bio-control, efficiency evaluation

CLC Number:

S476

SHA Yuexia, ZHANG Ang, WU Shunhua, SHEN Ruiqing. Screening and Efficiency Evaluation of Pseudomonas Strain in the Control of Magnaporthe oryzae[J]. Chinese Journal Of Biological Control, 2020, 36(2): 249-257.

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