Identification and Control Efficacy of Bacillus velezensis ZZBV-3 against Strawberry Root Rot

doi:10.16409/j.cnki.2095-039x.2021.02.005

Abstract

Abstract: In order to obtain excellent antagonistic bacteria against Fusarium oxysporum, a pathogen causes strawberry root rot, the soil from strawberry planting base was taken as antagonistic bacteria source, and the strains with the highest bacteriostatic rate were screened by indoor determination of their bacteriostatic activities. Then, the screened strains were classified and identified, and their bacteriostatic spectrum and control efficacy were evaluated. The results showed that total 39 strains were isolated from soil, among which 3 strains with strong inhibitory efficacy on F. oxysporum were obtained. Among them, strain ZZBV-3 had the strongest antibacterial activity, and its antibacterial diameter was up to 16.2 mm. According to morphological, physiological and polygenic (including 16S rDNA and gyrB genes) analyses, strain ZZBV-3 was identified as Bacillus velezensis. Strain ZZBV-3 had a wide antibacterial spectrum and has good antibacterial effect on various plant pathogens. The results of pots experiment showed that the control efficacy of strain ZZBV-3 on root rot of strawberry varieties ‘Hongyan’ and ‘Sweet Charlie’ were 77.96% and 81.18%, respectively, and the application prospect of biocontrol was acceptable.

Key words: Bacillus velezensis, screening and identification, strawberry root rot, Fusarium oxysporum, control efficacy

CLC Number:

YAO Jinai, HUANG Peng, LAI Baochun, YU Deyi. Identification and Control Efficacy of Bacillus velezensis ZZBV-3 against Strawberry Root Rot[J]. Chinese Journal of Biological Control, 2021, 37(1): 172-177.

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