Optimization of Fermentation Conditions of Bacillus amyloliquefaciens DP03-4,a Biocontrol Agent of Apple Tree Diseases

doi:10.16409/j.cnki.2095-039x.2022.04.013

Abstract

Abstract: A Bacillus amyloliquefaciens strain DP03-4 with biocontrol activity demonstrated to be broad-spectrum antagonistic toward several apple pathogens in field in previous study.In this study,the medium and fermentation conditions of the strain were optimized to increase its biomass and antagonistic activity by Orthogonal design.The optimized medium composition was yeast extract (1.5%),NaCl (0.25%) and glucose (1%).The optimized shake-culture conditions were temperature 30℃-35℃,medium volume 75 mL/250 mL,shake speed 210 r/min,initial pH value 6-7,and inoculation ratio 4%.Under the optimized culture conditions,the biomass and antagonistic activity of the fermentation broth were increased by 43.7% and 51.4%,respectively.The above results supplied information for further development and utilization of the strain.

Key words: Bacillus amyloliquefaciens, fermentation condition, orthogonal experiment design, biomass, antagonistic activity

CLC Number:

S476

WANG Cui, WANG Yan, DAI Pengbo, LIANG Xiaofei, ZHANG Rong, SUN Guangyu. Optimization of Fermentation Conditions of Bacillus amyloliquefaciens DP03-4,a Biocontrol Agent of Apple Tree Diseases[J]. Chinese Journal of Biological Control, 2022, 38(4): 904-910.

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