Identification of Antagonistic Bacteria against Angelica Wilt and Their Effect on Promoting Growth and Disease Controlling

doi:10.16409/j.cnki.2095-039x.2025.02.031

Abstract

Abstract: To clarify the classification status of antagonistic bacteria DST27 and HQT43 against Angelica wilt, evaluate their biocontrol effects, and provide theoretical basis for their development and utilization. The strain was identified using morphological characterization, physiological and biochemical traits, and 16S rDNA sequencing analysis. The potential of biocontrol bacteria to promote the growth of Angelica sinensis, prevent the illness known as Angelica wilt, and influence the kinds and numbers of soil microorganisms in the plant's rhizosphere were all investigated through pot experiments. In the early stage, strains DST27 and HQT43 with phosphorus solubilization, nitrogen fixation, and antibacterial functions were isolated from the rhizosphere soil of Codonopsis pilosula and Astragalus, preliminary identification of strain DST27 as Bacillus pumilus and HQT43 as Streptomyces albidoflavus. Significant differences (P＜0.05) were observed in the promotion effects of DST27 and its mixed inoculant (DST27:HQT43=1:1) on stem diameter, fresh weight, dry weight of aboveground part, fresh weight, and dry weight of underground portion. The findings demonstrated that the biocontrol agents' ability to prevent Angelica wilt outweighed their ability to treat it. The prevention effects of DST27, HQT43, and DST27+HQT43 (1:1) on Angelica wilt were 71.88%, 43.75%, and 73.53%, respectively, in the preventative group. The prevention and treatment effects in the treatment group were 65.62%, 34.38%, and 43.75%, in that order. Rhizosphere bacteria and actinomycetes dramatically increased following treatment with biocontrol bacteria, whereas the number of fungus significantly decreased. Actinomycetes and fungi in rhizosphere soil were most significantly affected by the mixed application of DST27+HQT43 at a 1:1 ratio. A good development and application prospect is presented by the combined application of DST27 and HQT43 in a 1:1 ratio, which may clearly alter the population of soil microorganisms and encourage crop growth in addition to having a good biocontrol effect.

Key words: Angelica wilt disease, Bacillus pumilus DST27, Streptomyces albidoflavus HQT43, strain identification, biocontrol

CLC Number:

S476

LI Zheng, WANG Yunyao, GUO Zengxiang, LUAN Hanyu, FU Yao, WANG Wei, JIN Ling, WANG Yan. Identification of Antagonistic Bacteria against Angelica Wilt and Their Effect on Promoting Growth and Disease Controlling[J]. Chinese Journal of Biological Control, 2025, 41(3): 561-570.

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