Welcome to Chinese Journal of Biological Control,Today is

Most Read

    Published in last 1 year |  In last 2 years |  In last 3 years |  All
    Please wait a minute...
    For Selected: Toggle Thumbnails
    Mating and Oviposition Behaviors and Rhythms of Delphastus catalinae (Horn)
    LIU Qi-quan1,2, WANG Zhu-hong1, XU Gui-ping1, XIN Xiao-yang1, HUANG Jian1
      
    Abstract2434)      PDF(pc) (402KB)(1079)       Save
    Mating and oviposition behaviors and rhythms of Delphastus catalinae (Horn) were observed, and the influence of mating frequency on fecundity was investigated. Results showed that mating was a male-dominated phenomenon. When sexually mature, the males initiated mating. A complete mating process took 18.6 min on average. A pre-mating period of 5.0±0.5 d for the male and a pre-ovipositing period of 5.8±0.4 d for the female were observed. Multiple mating occurred during the whole day and peaked in the early morning at 6:00-8:00. After mating, female adults laid more eggs during the day than at the night. Oviposition lasted 63-120 d with 353-413 eggs laid per female. With the increase of the mating frequency, oviposition period and fecundity increased but longevity of female decreased. Multiple mating added to the reproductive performance of D. catalinae.
    Related Articles | Metrics | Comments0
    Isolation of Antagonistic Actinomyces against Banana Fusarium Wilt Disease and Primary Evaluation on Their Inhibition Effects
    QIN Han-chun1,2;YANG La-ying1;LI Song-wei1,2;XIE Yu-ping1;HUANG Jun-sheng1
      
    Abstract2320)      PDF(pc) (383KB)(975)       Save
    Banana Fusarium wilt disease is a systemic vascular disease caused by Fusarium oxysporum f.sp. cubense. In this paper, 139 antagonistic actinomyces strains were isolated from the rhizosphere soil sampled from Dongfang, Basuo, Huangliu, Sanya in Hainan province and Zhanjiang, Xuwen, Hai'an in Guangdong province. Through Disc diffusion method, 8 strains were demonstrated to be antagonistic against F. oxysporum f.sp. cubense race 4. Further experiments showed that 4 strains could not only stably inhibit the mycelial growth of race 4, but also inhibit the other 14 different isolates of F. oxysporum f.sp. glycinea. In addition, the fermentation supernatant from the 8 actinomyces strains could inhibit the spores germination of F. oxysporum when mixed together for 12 h. And 6 of them inhibited by more than 85%. Pot experiments showed that 2 strains had good control effect on banana Fusarium wilt disease by over 86%, very significantly higher than that of hymexazol treatment.
    Related Articles | Metrics | Comments0
    Cited: Baidu(10)
    A FIELD SURVEY ON THE PARASITOIDS OF RICE HESPERIIDS IN SHAXIAN COUNTY, FUJIAN PROVINCE
    XIE Ming
      
    Abstract2124)      PDF(pc) (194KB)(756)       Save
    Four species of egg parasitoids of the rice hesperiids were recorded in the field surveys conducted in Shaxian County, Fujian Province during August-November, 1982. Trichogramma confusum, T.japonicum and Telenomus sp. were the predominant species. Exposing the eggs of the skippers in the field during the same period resulted in an average parasitization rate of 10.4% (ranging 5.3-14.75), During October to November, the parasitization rate of the skippers on the weeds at edges of the rice fields was 26.6% (ranging 10-39.3%).
    Related Articles | Metrics | Comments0
    Isolation and Identification of Entomopathogenic Fungi Naturally Infecting Solenopsis invicta and Their Virulence to S. invicta
    LIU Xiao-yan1;Lü Li-hua2;HE Yu-rong1
      
    Abstract2103)      PDF(pc) (110KB)(1069)       Save
    Red imported fire ant (RIFA), Solenopsis invicta, was one of the most dangerous and devastating pests in the world. In this study, pathogenic fungi were isolated from samples of RIFA collected from Guangzhou, Huizhou, Shenzhen, etc, in Guangdong province. Isolate PL was found to be virulent to RIFA and identified as Paecilomyces lilacinus according to its morphological characteristics. Among the 10 strains of PL isolated by single-spore method, strain PL04 had the highest virulence to RIFA with accumulative mortality of 70.60% on the 15th d after treatment with 1×108 conidia/ml.
    Related Articles | Metrics | Comments0
    Cited: Baidu(5)
    Correlation between Biological Characteristics of Beauveria bassiana and Its Virulence to Plutella xylostella
    LEI Yan-yuan1;Lü Li-hua2;HE Yu-rong1;CHEN Dong-hong1
      
    Abstract2026)      PDF(pc) (341KB)(1046)       Save
    To explicit the correlation between biological characteristic and virulence of Beauveria bassiana to Plutella xylostella, five B. bassinana strains, including parental strain GXU-Bb and its four single-spore isolations were assayed on the growth rate, sporulation, spore germination rate, extracellular protease level, chitinase level and their virulence to the second instar larvae of P. xylostella. Results showed there was no significant difference in growth rate and spores germination rate among the strains. The sporulation of these strains was 1.54×107-2.98×107 conidial/ml. The extracellular protease level and chitinase level were 1.04-1.19 and 1.05-1.15, respectively. The corrected mortality of each strain to the second instar larvae was 39.81%-83.79%. Factors affecting the virulence were analyzed based on the correlation coefficient (r). The virulence of B. bassiana strains against P. xylostella was not affected by the growth rate and spore germination rate, but was positively correlated with the sporulation, extracellular protease level and chitinase level.
    Related Articles | Metrics | Comments0
    Cited: Baidu(18)
    Results of Using BtMP-342 to Control Helicoverpa armigera and Plutella xylostella
    YANG Zi-wen;XIE Tian-jian;ZHONG Lian-sheng;WANG Kai-mei;ZHEN Da;
    HUANG Min-song;LIU Xue-tang;SHAO Xu-dong;ZHAO Ru-shan;QIAO Jian-zhong;LI Guo-zeng;SU Ping-shun;LIU Hai-ying;NIE Zhi-ying;LI Hong-ru
      
    Abstract1890)      PDF(pc) (181KB)(798)       Save

    Field experiments were carried out at Henan, Hebei and Hubei to control Helicoverpa armigera and Plutella xylostella with Bt MP-342. MP-342 dust (16000IU/mg) and MP-342 solution (4000IU/ml), produced by Hubei Bt Research and Development Center, were used to prepare Bt sprays for field application. Experimental results showed that the MP-342 dust 500× and 1000× sprays killed the H. armigera 75.0%-95.7% and 72.2%-85.9% respectively in 70 h. The control effect was significantly higher than that of the chemical pesticide treatments. The MP-342 solution 1000× and 2000× sprays reduced the field population of the P. xylostella 98.5% and 88.4% respectively in 48 h.

    Related Articles | Metrics | Comments0
      
    Abstract1885)      PDF(pc) (67KB)(966)       Save
    Related Articles | Metrics | Comments0
    Secondary Metabolites Related with Plant Resistance against Pathogenic Microorganisms and Insect Pests
    LI Ming1,2,3;ZENG Ren-sen2;LUOShi-ming2
      
    Abstract1879)      PDF(pc) (199KB)(1496)       Save
    Related Articles | Metrics | Comments0
    Cited: Baidu(20)
    Insect Natural Enemies of Tea Pests in Hunan
    CHEN Chang-ming;SONG Hui-ying;XIAO Tie-guang
      
    Abstract1867)      PDF(pc) (87KB)(846)       Save
    A survey on the insect natural enemies of tea pests was carried out in
    Hunan province, 1988~ 1992. More than 95 species which belong to the Hy-menoptera, Coleoptera, Diptera and Neuroptera were found. Some of them were very important insect natural enemies of the major tea pests. The problems of biological control in the tea ecosystem is discussed.
    Related Articles | Metrics | Comments0
    Cited: Baidu(5)
    STUDY ON BIOLOGICAL CONTROL OF GRASSLAND LOCUST BY PSEUDOMONAS PSEUDOALCALINGENES
    YANG Zhi-rong1; ZHU Wen1; GE Shao-rong1; HOU Ruo-tong1; LIU Shi-gui1; WANG Zhi-gang2; LI Tao3; YAN Sheng-san3; WANG Gui-ling3
      
    Abstract1843)      PDF(pc) (141KB)(697)       Save
    Biological assay showed that Pseudomonas pseudoalcalingenes isolated fromnatural dead corpse of Ceracris kiangsu was found effective against the major species of grassland locusts in Qinghai and Sichuan. The lethal rate of Aulacobothrus sinensis was 80.3 % when treated with 10~(10) bacteria/ml of P. pseudoalcalingenes. The lethal rates showed eminent differences between different species of locust. The lethal rate to Locusta migratoria manilensis was 48%, The fungus could also infect Gynaephora ruoergensis, Lencania separata and Maladerd orientails to some extent. Application of P. pseudoalcalingenes in the field to control locusts had an efficiency of more than 80%.
    Related Articles | Metrics | Comments0
    Cited: Baidu(7)
    Inhibitory Effect of Metschnikowia zizyphicola XY201 on Fungal Pathogens of Postharvest Jujube Fruits
    SHI Jun-feng;XUE Meng-lin;ZHANG Xiao-yu;WANG Liang;WANG Chun-sheng
      
    Abstract1829)      PDF(pc) (566KB)(1009)       Save
    Culture time for maximum biomass, activity under different environmental conditions and the inhibitory effect of Metschnikowia zizyphicola XY201 against the four fungal pathogens of postharvest jujube fruits were investigated in this study. Results showed the maximum biomass (3.84×108 cfu/ml) of strain XY201 in NYDB broth emerged after 96 h incubation. Viability of strain XY201 varied under different temperatures and media. Strain XY201 cultured in liquid medium at 0℃ kept the highest viability and concentration of XY201 remained 6.46×107 cfu/ml after 240 d incubation. Strain XY201 significantly inhibited spore germination and hyphal elongation of Penicillium expansum, Alternaria alternate, Aspergillus niger and Rhizopus nigricans when co-cultured in PDB. M. zizyphicola XY201 at the concentration of 5×108 cfu/ml completely inhibited the growth of the four fungal pathogens. Inhibitory activity of strain XY201 against A. alternate was 42.59% by dual culture on PDA medium.
    Related Articles | Metrics | Comments0
    Cited: Baidu(6)
    INCIDENCE OF BACILLUS THURINGIESIS IN DIFFERENT TYPES OF SOIL AND VEGETATION IN HEBEI PROVINCE
    FAN Xiu-hua; FENG Shu-liang; XING Jian-min; FU Yun-qin; WANG Rong-yan
      
    Abstract1816)      PDF(pc) (181KB)(801)       Save
    241 strains of Bacillus thuringiensis were isolated from 4637 samples of 148 counties belonging to six ecological regions of Hebei Province. The results showed that the incidence of B. thuringiensis at different soil subclass and different vegetation varied greatly. The highest isolation rate was from the meadow drab soil (14%), the lowest was from chestnut soil and damp soil (2%), and none was isolated from the saline soil and sandy soil. For the vegetation, the highest isolation rate was from cotton field (13.4%), and only 20% from sesame and naked oats field.
    Related Articles | Metrics | Comments0
    Influence of Temperature on Functional Response of Asecodes hispinarum TANG Chao, PENG Zheng-qiang, CHEN Kai-ning, L Bao-qian,
    TANG Chao;PENG Zheng-qiang;CHEN Kai-ning;LU Bao-qian;JIN Qi-an;ZHOU Jin-xia;FU Yue-guan;WAN Fang-hao
      
    Abstract1795)      PDF(pc) (122KB)(798)       Save
    Functional response of Asecodes hispinarum to the increase of larval density of Brontispa longissima was examined at temperatures 16, 20, 24, 28 and 32℃. The results showed that, the functional response fitted well by Holling's disc equation at temperature regime of 16-32℃. The rate of successful attack rose with increase of temperature, reaching the highest at 28℃ and then decreasing significantly. There was no influence of temperature on handing time. After exposed to various densities of Brontispa longissima 4th instar larvae for 24 h, some females failed to parasitize host larva. The percentage of females that parasitized at least one Brontispa longissima larva increased with the increase of host density.
    Related Articles | Metrics | Comments0
    Inhibitory of an Antifungal Protein Produced by Bacillus subtilis C-D6 against Magnaporthe oryzae
    JIA Shu-juan1,2, ZENG Da-xing2, WU Xiao-li1,2, TU Guo-quan1
      
    Abstract1791)      PDF(pc) (363KB)(1062)       Save
    Bacillus subtilis C-D6 and its culture filtrate showed obvious antifungal activity against Magnaporthe oryzae in dual plate. An antifungal protein (AFP), inhibitory to the formation of appressorium of M. oryzae, was isolated from C-D6 culture filtrate by using ammonium sulfate precipitation at 20% saturation and Sephadex G-75 column chromatography. SDS-PAGE analysis demonstrated that the protein was a monomer with molecular weight of 32 kD. Light microscopic observation showed that spore of M. oryzae treated with AFP formed bulbous germ tube and hypochromic swollen appressoria, and further development of germ tube was inhibited. Inhibitory activity of the AFP was stable at 80 ℃ for 30 min and in pH value ranged from 5.6 to 9.3.
    Related Articles | Metrics | Comments0
    EFFECTS OF 3 HERBICIDES ON MORTALITY OF EGGS, LARVAE, PUPAE AND ADULTS OF WATER HYACINTH WEEVIL, NEOCHETINA EICHHORNIAE WARNER (COL.: CURCULIONIDAE)
    DING Jian-qing; WANG Ren; WANG Nian-ying; FU Wei-dong; CHEN Zhi-qun
      
    Abstract1788)      PDF(pc) (186KB)(973)       Save

    The effects of 3 herbicides, Glyphosate, Paraquat and Londex at recommended herbicidal rates on the mortality of water hyacinth weevil, Neochetina eichhorniae were tested in both laboratory and field. The hatching rates of the eggs contacted directly with Glyphosate, Parapuat, Londex and water (control) were 83.3%, 93.3%, 92.2% and 95.6%, respectively. When these herbicides were sprayed on the water hyacinth leaves infested with weevil eggs, the hatching rates were 90.0%, 53.4%, 85.6% and 93.6%, respectively. There were no great effects on the mortality of the first instar larvae and pupae of the weevil treated with the herbicides. The eggs oviposited by each female/d treated with Glyphosate, paraquat, londex were 1.14, 0.17, 0.27, the control being 1.13. The survival rate of the adults feed on the leaves sprayed with 3 herbicides and water were 92.5%, 0, 95.0% and 97.5% after 70d, respectively. The order of effect of the 3 herbicides on the weevil was: Paraquat>Londex>Glyphosate.

    Related Articles | Metrics | Comments0
    Cited: Baidu(9)
    Bioactivity of Soil-dwelling Entomopathogenic Fungi against Tessaratom apapillosa (Hemiptera: Pentatomidae)
    FAN Ji-qiao1, AN Xin-cheng2, XIA Jin-ming1, HU Qiong-bo1
      
    Abstract1776)      PDF(pc) (154KB)(935)       Save
    Litchi stink-bug, Tessaratom apapillosa Drury, often cause serious damage on litchi, Litchi chinensis and longan, Euphoria longan, which are common varieties in orchard of South China. It is urgent to develop valid biological agent for control the pest and improve IPM strategy on orchard farming. Entomopathogenic fungi was regarded as a vital ecological factor pressing pest populations in field, however, there were few researches conducted on entomopathogenic fungi against litchi stink-bug. In this study, strains or isolates of Beauveria bassiana, Metarhizium anisopliae, Isaria fumosorosea and Isaria javanicus were isolated from soil samples of litchi orchards in South China. Virulence of forty strains was assayed for their efficacies against T. apapillosa. Two strains, Bb07 and Ma03 demonstrated high toxicity, LC50 values at 14 d were 2.20×106 conidia·mL-1 and 2.34×106 conidia·mL-1, respectively, and LT50 values at 1×107 conidia·mL-1 were 6.1 d and 6.6 d, respectively. Furthermore, when Bb07 and Ma03 were sprayed at a concentration of 1×108 conidia·mL-1 in field trials, they achieved efficiencies of 90.78% and 90.34%, respectively.
    Related Articles | Metrics | Comments0
    INDIRECT CONTROL OF PINE WOOD NEMATODE BY RELEASING SCLERODERMA GUANI
    CHEN Mu-rong; SONG Shi-han; ZHANG Lian-qin; ZHANG E; LI Qiao-hua; YANG Liang
      
    Abstract1756)      PDF(pc) (194KB)(819)       Save
    23 test points were set up at 7 releasing regions including Shenzhen Forest Farm in June, 1994 and a total about 500000 parasitic wasps, (Scleroderma guani) were released on to pine forest to control the pine sawyer (Monochamus alternatus Hope), a vector of pine wood nematode. The parasitic rate was 25.6%~46.9% averaged 35.2%, in the first generation of the wasp covering an area about 18 ha. Owing to the multiplication and expansion of this wasp in the forest, the controlled area reached up to 162.5ha. in October and November and the withering rate of pine trees caused by pine wood nematode disease was about 2.63%, while that in the check regions where no wasp has been released was about 10.33%. The control effect counted as 67. 99%~86.59%.
    Related Articles | Metrics | Comments0
    STUDIES ON TRICHOGRAMMA PINTOI [HYM.:TRICHOGRAMMATIDAE]: ITS DEUTEROTOKOUS REPRODUCTION, ARTIFICIAL PROPAGATION AND FIELD RELEASES
    WANG Fu-cheng; ZHANG Shu-yi
      
    Abstract1755)      PDF(pc) (165KB)(799)       Save
    Two years of laboratory experiments confirmed that T. pintoi conducted parthenogenetic reproduction producing progenies of both sexes. Over 90X of the offsprings were females, which was similar to that occurred in its sexual reproduction. In Yuncheng, the natural hosts of T. pintoi included cotton boll worm, Heliothis armigera. and turnip moth, Agrotia segetum. In the laboratory rearings, the parasitoid parasitized eggs of satin moth, Leucoma salicis. and several noctuids, but did not oviposit in the eggs of oak silkworm, Antheraea pernyi. and eri-silkworm, Philoaamia cynthia ricini. Egg of satin moth was found to be a suitable host for easy and efficient mass propagations of T. Pintoi. Inundative releases of L. pintoi and the more conmmonly used T. dendrolimi to control cotton bollworm in the field plots resulted 63.7% and 47.9% host parasitization, respectively. The high female rate in the parasitoid's parthenogenetic reproduction is considered advantageous for its use in controlling cotton bollworm populations, and merits further studies.
    Related Articles | Metrics | Comments0
    Induction of Systemic Resistance in Muskmelon against Phytophthora melonis in Fields
    WANG Rui-ping
      
    Abstract1755)      PDF(pc) (124KB)(804)       Save
    The biotic and chemical agents were used respectively to induce the systemic resistance in muskmelon (Cucumis melo L ) in fields of Hami in Xinjiang province in 1996 and 1997 The induced resistance in the melon successfully prevented from Phytophthora melonis infection in nature for four weeks In contrary, plants in CK suffered a lot compared to the treated plants There were no differences between treated and CK plants in respect of growth and fruit-bearing
    Related Articles | Metrics | Comments0