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Published in last 1 year |  In last 2 years |  In last 3 years |  All Please wait a minute... For Selected: Download Citations EndNote Ris BibTeX Toggle Thumbnails Select Progress and Prospect of Microbial Metabolite Pesticides Research, Development and Application in China HE Ya-Wen, LI Guangyue, TAN Hong, KANG Qianjin, GE Beibei, ZHAO Yangyang, ZHANG Kecheng, JIANG Xiliang, LIU Fengquan, LI Yaning, ZHANG Hongyan, BAI Lingquan, XIANG Wensheng, QIU Dewen, YANG Ziwen, DENG Zixin Chinese Journal of Biological Control    2022, 38 (3): 537-548.   DOI: 10.16409/j.cnki.2095-039x.2022.02.028 Abstract （818）      PDF（pc） （1924KB）（1757）       Knowledge map    Save Microbial metabolite pesticide (MMP) contains the microbial fermentation metabolite as the main active component and is effective in the prevention of both plant disease and weed problems or in the promotion of plant growth and development. MMPs mainly include the agricultural antibiotics, plant immune elicitor and plant growth regulator, they are the most widely used biopesticides in China. MMP has both preventive and therapeutic effects on plant disease, therefore, it is one of the important areas for the future development of green pesticides. In this review we summarize the major MMPs, their characteristics, and latest research and development progress. We then discuss the problems, challenges and prospects of MMP research, development and application in China, and provide a reference for the future research and development of new MMP. Reference | Related Articles | Metrics | Comments（0） Select Development and Application of Insect Sex Pheromone Technology in China LIU Wancai, LIU Zhendong, ZHU Xiaoming, DU Yongjun Chinese Journal of Biological Control    2022, 38 (4): 803-811.   DOI: 10.16409/j.cnki.2095-039x.2022.05.010 Abstract （733）      PDF（pc） （480KB）（1490）       Knowledge map    Save Insect sex pheromones are trace and volatile chemicals emitted by pheromone glands during calling after sexual maturation,which attract conspecific individuals to mate and are species specific.It has been widely applied in the monitoring and control of insect pests.In China,a series of great progresses have been made in the industrialization of chemical synthesis and purification,improvement of the techniques for stabilization and controlled release of pheromone compounds,the development of various traps and smart pheromone trapping and forecasting technology,and the practical application of pheromone trapping and mating disruption for various pests in the field,which meets the needs of monitoring and green control of major pests.Here,we comprehensively introduced and summarized the principle,types and application methods of pheromone lures and traps of mass trapping and dispensers of mating disruption developed in China,and their application in the field.The relationship between calling and mating,the attractiveness and specificity of sex pheromone,and the effects of biotic and abiotic factors on pheromone trapping and mating disruption were systematically summarized.The case analysis of application in important pests of vegetables,fruit trees,cotton and other crops was presented in detail.The problems found in the research and development,extension and application of pheromone technology were discussed and analyzed,and countermeasures and suggestions for improving the work were put forward. Reference | Related Articles | Metrics | Comments（0） Select The Development of Biological Pesticides Based on RNA Interference GAO Liwen, CHEN Shiguo, ZHANG Yu, QIANG Sheng Chinese Journal of Biological Control    2022, 38 (3): 700-715.   DOI: 10.16409/j.cnki.2095-039x.2021.11.015 Abstract （730）      PDF（pc） （511KB）（2285）       Knowledge map    Save RNA interference, also known as post transcriptional gene silencing, is an emerging new genetic engineering technique that can effectively silence or inhibits the expression of target genes. Biopesticides based on RNA interference are considered as a revolutionary technique in the field of plant protection in the future, which will completely change the ideas and strategies of controlling agricultural diseases, insects, weeds and other pests. Here, the basic principle and development process of RNA interference were briefly reviewed. The current status of research and application of RNAi biopesticides was summarized. The opportunities and challenges to develop RNAi biopesticides as well as its development prospect were deeply analyzed. This review serves to provide some valuable references for the development of RNAi biopesticides in China. Reference | Related Articles | Metrics | Comments（0） Select The Research Progress on the Effects of Entomopathogenic Fungi on Natural Enemies YAN Sen, REN Xiaoyun, WANG Dengjie, ZHANG Ye, ZHANG Zhike, GUO Jiyuan, WANG Haihong, LEI Zhongren, WU Shengyong Chinese Journal of Biological Control    2023, 39 (1): 221-230.   DOI: 10.16409/j.cnki.2095-039x.2022.01.014 Abstract （688）      PDF（pc） （757KB）（1822）       Knowledge map    Save Entomopathogenic fungi, the important microbial insecticides, have been widely used in biological control of agricultural and forestry pests. Because of the wide range of hosts and active infection, entomopathogens, when infecting or killing the target pests, may have potential pathogenic or sublethal risks to non-target arthropods including insect natural enemies. In this paper, we summarized the progress in application of entomopathogens for pest control and the negative effects of entomopathogens on natural enemies, and provided perspectives on the future development direction of combined application of the high potential entomopathogenic fungi pesticides and natural enemy organisms in pest management. Reference | Related Articles | Metrics | Comments（0） Select Summary of Research on Insect Chitinase and Its Application LI Chunchun, GOU Yuping, ZHANG Kexin, ZHANG Qiangyan, LIU Changzhong Chinese Journal of Biological Control    2022, 38 (4): 1020-1029.   DOI: 10.16409/j.cnki.2095-039x.2021.09.023 Abstract （670）      PDF（pc） （745KB）（1711）       Knowledge map    Save Chitin,a nitrogen-containing polysaccharide biological polymer in nature,is the main structural component of insect epidermis,midgut peritrophic membrane and tracheal system,protecting insects from chemical erosion,physical damage,and pathogen invasion.Recently,more and more attention is paid to insect chitinase for its role in pest control,for it could degrade chitin in insect body wall and peritrophic membrane.By regulating chitinase,the growth and development of insects will be affected directly and even be controlled effectively.This paper summarized the structure,gene expression characteristics,physiological functions,and influence factors of insect chitinase,as well as its application and shortcomings in pest control,in order to provide a scientific basis for the further development and application of insect chitinase in the field of plant protection. Reference | Related Articles | Metrics | Comments（0） Select Synergistic Effect of Zhongshengmycin and Natamycin on Tomato Gray Mold JI Lindong, MA Chao, XU Jun, ZHAO Yijun, ZHANG Yanhui, DUAN Xiaoli, YANGYang, CHEN Chang Chinese Journal of Biological Control    2022, 38 (4): 874-879.   DOI: 10.16409/j.cnki.2095-039x.2022.04.005 Abstract （561）      PDF（pc） （389KB）（615）       Knowledge map    Save In order to improve the control effect of existing bio-pesticides on tomato gray mold,the inhibitory activities of mixtures containing Zhongshengmycin and Natamycin on mycelial growth and conidial germination of Botrytis cinerea were investigated,and the synergistic effect and appropriate ratio of the two pesticides were determined.The mixtures of Zhongshengmycin and Natamycin showed synergistic effect on inhibition of hyphae growth when their weight ratios were at 9:1 and 1:1,with the synergistic coefficient of 3.6348 and 1.7145,respectively.Synergistic effect was also expressed in inhibition of the conidial germination when their weight ratios were at 9:1,7:3 and 1:1,with the synergistic coefficient of 1.7268,1.6335 and 1.5336,respectively.Field experiments showed that the 500 x dilution of 3% wettable compound powder of Zhongshengmycin and Natamycin (9:1) performed good effects on tomato gray mold on leaves and fruits,with the control efficacies of 81.04% and 78.48%,respectively,significantly higher than that of individual ingredient.The above results showed that the mixture of Zhongshengmycin and Natamycin in proper proportion could effectively improve the control effect on tomato gray mold. Reference | Related Articles | Metrics | Comments（0） Select Synergistic Effect of a Biocontrol Bacterium D25 and Cyprodinil·Fludioxonil on the Control of Tomato Gray Mold HUANG Xin, ZHENG Lining, GU Xuehu, XU Xuanwei, ZHANG Hao, WANG Yingchun Chinese Journal of Biological Control    2022, 38 (4): 860-867.   DOI: 10.16409/j.cnki.2095-039x.2022.04.018 Abstract （547）      PDF（pc） （385KB）（805）       Knowledge map    Save In order to screen the fungicide complex composed of biocontrol bacterium D25 and chemical fungicide to improve the biocontrol efficacy on tomato gray mold as well as reducing the dosage of chemical fungicides,toxicity of 12 fungicides against Botrytis cinerea was firstly tested in vitro by toxic medium method Bio-compatibility between fungicides with strong antifungal activity and biocontrol bacterium D25 was determined by plate counting method.O ptimal ratio of fungicides and biocontrol bacteriun D25 was screened by the toxicity ratio,and control efficacy of the complex in suppressing tomato gray mold was further tested in pot experiment.The results showed that among 12 fungicides,Bacillus subtilis and Trichoderma harzianum had the strongest toxicity against B.cinerea,with EC50 values of 4.564×10-5 mg/L and 0.021 mg/L,respectively.Cyprodinil·fludioxonil had better toxicity,with EC50 value of 0.043 mg/L.Two botanical fungicides,carvacrol and matrine·osthol,had the lowest toxicity,with EC50 values of 12.469 mg/L and 19.220 mg/L,respectively.Biocompatibility test showed that no negative effect was found between cyprodinil·fludioxonil and bio-control bacterium D25.The complex of cyprodinil-fludioxonil (0.043 mg/L) and bio-control bacterium D25(7×104CFU/mL) at a volume ratio of 5:5 performed synergistic effect,with the toxicity ratio of 1.3943.In the pot experiment,the control efficacy of the prevention group reached 72.29%,which was higher than that of the individual fungicide by 19.26% and 29.29%,respectively,while the control efficacy of the treatment group reached 61.57%,which was higher than that of the individual fungicide by 15.40% and 21.23%,respectively.In conclusion,combined application of bio-control bacterium D25 and cyprodinil·fludioxonil had a synergistic effect against B.cinerea,which reduced the application amount of cyprodinil·fludioxonil by 50% without reducing the control efficacy on tomato gray mold,and had great potential to be applied in field. Reference | Related Articles | Metrics | Comments（0） Select Advances in the Development of Spray-Induced Gene Silencing for the Management of Plant Pathogenic Fungi MO Qin, JIANG Wei, CHEN Yifan, LÜ Beibei Chinese Journal of Biological Control    2022, 38 (5): 1316-1324.   DOI: 10.16409/j.cnki.2095-039x.2022.06.005 Abstract （526）      PDF（pc） （692KB）（823）       Knowledge map    Save Fungal pathogens are the largest group of plant pathogens in agriculture,which continuously lead to serious yield loss of crops,fruit and vegetables worldwide.At present the control of pathogenic fungi mainly rely on the extensive use of chemical fungicides,which also brings us several concerns such as resistance to fungicides,food safety and ecological environment pollution.Thus,it is urgent to develop a green management strategy for pathogenic fungi in agriculture.Spraying induced gene silencing (SIGS) technology is a new potential bio-control technology in field application by using specific double-stranded RNA or small interfering RNA to control targeted genes of pathogens,which has been successfully applied to control numerous pathogenic fungi.Here,we focused on the advances in the development of SIGS mechanism and its applications on the management of plant pathogenic fungi,which might be helpful to the development of plant pathogenic fungi-targeted bio-pesticides based on SIGS. Reference | Related Articles | Metrics | Comments（0） Select Research Progress in the Functions and Mode of Actions of Insect Adipokinetic Hormones ZHENG Hongyuan, FAN Shufan Chinese Journal of Biological Control    2022, 38 (3): 689-699.   DOI: 10.16409/j.cnki.2095-039x.2021.07.015 Abstract （477）      PDF（pc） （522KB）（795）       Knowledge map    Save Adipokinetic hormone (AKH) is one of the most important neuropeptides regulating energy homeostasis in insects by inducing mobilization of energetic substrates in the fat body to fuel energy-consuming activities. Early studies on AKH had focused on unveiling the functions and signal transduction of AKH on promoting catabolism of nutrient reserves, such as triacylglycerol and glycogen, during insect locomotion including flight and walking. Recent studies have illustrated that AKH/AKH receptor system is involved in mobilization of nutrient stores during insect vitellogenesis and lactation. Additionally, AKHs also play important roles in homeostasis maintenance under stress conditions including antioxidant, starvation, and immunity. Accumulating evidence suggests that, as a key hormone for mobilizing energy stores, AKH is a pleotropic factor involved in many physiological events. Here we reviewed the functions and modes of action of AKHs in insect locomotion, reproduction and stress resistance, aiming to support further uncovering the molecular mechanism of AKH signaling pathway in regulation of insect energy homeostasis, and elaborating genetic basis of long-distance migration, high fertility and anti-stresses in pests. Reference | Related Articles | Metrics | Comments（0） Select Determination of Larval Instars and Duration of the Tomato Leaf miner, Tuta absoluta (Meyrick) (Lepidoptera:Gelechiidae) ZHANG Guifen, YIN Huijun, WANG Yusheng, LI Zhenjiang, BI Siyan, WANG Rui, LIU Wanxue, WAN Fanghao Chinese Journal of Biological Control    2023, 39 (2): 340-345.   DOI: 10.16409/j.cnki.2095-039x.2022.03.024 Abstract （464）      PDF（pc） （383KB）（843）       Knowledge map    Save The tomato leafminer Tuta absoluta (Meyrick), originated in Peru, South America, is a worldwide destructive pest of tomato. In China, T. absoluta was first detected in Xinjiang in 2017 and has posed a potential threat to China’s tomato and other grain and vegetable solanaceous crops. Division of larval instars of the leafminer is important for studies of biological characteristics, population dynamics, and population monitoring and prediction and control, such as biocontrol based on larval parasitoids. The head capsule width is an important index for larval instar division. The number of larval instars was determined and tested according to the frequency distribution analysis, Dyar’s rule, Crosby’s growth rule, as well as regressive analysis based on two measured parameters, the head capsule width and body length. The head capsule width of the leafminer larvae fitted well with the Dyar’s rule and Crosby’s growth rule. There was an obvious exponential regression relationship between the number of larval instars and the head capsule width, showing that the head capsule width can be used as an important index for larval instar division. The larvae of T. absoluta can be divided into four instars. The values of the head capsule width of the 1st, 2nd, 3rd, and 4th instar larvae were 0.1375―0.1575 mm, 0.2125―0.2725 mm, 0.3200―0.4100 mm, and 0.4975―0.5800 mm, respectively. The values of body length corresponding to the above four larval instars were 0.80―1.52 mm, 0.91―3.33 mm, 2.42―4.89 mm, and 4.18―7.32 mm, respectively, which could be used as an auxiliary index to determine the number of larval instars of the tomato leafminer. At 27℃, the larvae completed development in 13 d, the 1st, 2nd, 3rd, and 4th larval instars lasted 1―4 d, 3―6 d, 4―7 d, and 6―15 d, respectively. Thus, the T. absoluta larvae are divided into four instars according to the head capsule width, while the body length can be used as an auxiliary index in larval instar division duo to its great variation in the same and between instars. Reference | Related Articles | Metrics | Comments（0） Select Mechanism of Chaetomium globosum in Promoting Growth and Preventing Disease and Advance of Its Application and Research FENG Chaohong, LI Lijuan, ZHANG Jiaojiao, WANG Junmei, SONG Yuli, LI Haohai, XU Fei Chinese Journal of Biological Control    2023, 39 (4): 961-969.   DOI: 10.16409/j.cnki.2095-039x.2023.02.043 Abstract （452）      PDF（pc） （432KB）（6056）       Knowledge map    Save Chaetomium globosum, one of common plant endophytic fungi, possesses potential biocontrol function against many plant diseases. This paper summarized the research advances of Chaetomium globosum on biocontrol effect, and its biocontrol mechanisms, which include antibiosis, growth-promoting effect, induction of plant resistance, niche and nutrient competition, and hyperparasitism. Chaetomium globosum exhibits great application potential on plant disease control and growth promotion, soil improvement, and straw degradation. Advances on the fermentation technology and genetic engineering were also illuminated. This study will provide theoretical references for the application research of Chaetomium globosum as biocontrol agent. Reference | Related Articles | Metrics | Comments（0） Select Potential and Prospect of Beauveria bassiana and Metarhizium anisopliae as Biological Pesticides for the Control of Red Fire Ants NONG Xiangqun, WANG Guangjun, WANG Yiyan, ZHANG LEI, GAO Qionghua, YU Yonghao Chinese Journal of Biological Control    2023, 39 (2): 453-461.   DOI: 10.16409/j.cnki.2095-039x.2023.01.004 Abstract （443）      PDF（pc） （519KB）（880）       Knowledge map    Save The red fire ant Solenopsis invicta Buren has invaded from South America into many countries in North America, Asia and the Pacific islands. In China, the ant has spread to more than 500 cities and counties of 12 provinces, seriously damaging agricultural and forestry production, ecological balance and human settlement safety. In order to achieve sustainable management of red fire ants, it is necessary to introduce and strengthen the role of biological control. Here we summarized the overall situation of development of entomopathogenic fungi Beauveria and Metarhizium as biological pesticides, as well the detailed progress of the development directing at red fire ants, including highly virulent strains, large-scale production, product formulations and registration, and the application in the field. Meanwhile, we analyzed the advantage and problems of the two fungi for control of red fire ants. The prospect outlook of fungal insecticides in the control of red fire ants was discussed. Reference | Related Articles | Metrics | Comments（0） Select Application of Digital PCR in the Detection of Genetically Modified Plants YOU Yinghua, ZHAO Zhi, LI Yanan, SONG Zhiguo, CHANG Honglei Chinese Journal of Biological Control    2022, 38 (5): 1143-1148.   DOI: 10.16409/j.cnki.2095-039x.2022.09.012 Abstract （443）      PDF（pc） （620KB）（649）       Knowledge map    Save In response to the growing plantation of genetically modified plants,new demands for detecting transgenic components have arisen.Recent years have witnessed a rapid increase in the use of digital PCR to detect transgenic components as a new molecular biology technology.This paper introduces the concept of digital PCR as well as its advantages in the detection of transgenic components.It also summarizes the application of digital PCR in quantitative analysis of transgenic components,preparation of reference materials,and determination of foreign gene copy numbers in genetically modified plants. Reference | Related Articles | Metrics | Comments（0） Select Preliminary Study on the List Management of Registered Varieties of Biopesticides in China YANG Jun, HOU Yanhua, LIN Ronghua, JIANG Hui, LIU Yinchi, QIU Lihong, LIU Xue Chinese Journal of Biological Control    2022, 38 (4): 812-820.   DOI: 10.16409/j.cnki.2095-039x.2022.05.011 Abstract （414）      PDF（pc） （455KB）（1043）       Knowledge map    Save In recent years,new varieties of biopesticides have emerged thick and fast in China,the registration heat of biopesticides has continued to rise,and the registration management of it has been constantly standardized.This paper discusses the list management of registered varieties of biopesticides in China,puts forward the scope and establishment principles of the list,combs and forms the list of registered varieties of biopesticides in China.In addition,this paper puts forward suggestions on improving the list management of registered varieties of biopesticides. Reference | Related Articles | Metrics | Comments（0） Select Advances on the Pathogenic Mechanism of Entompathogenic Nematodes CHANG Doudou, WANG Congli, LI Chunjie Chinese Journal of Biological Control    2022, 38 (5): 1325-1333.   DOI: 10.16409/j.cnki.2095-039x.2021.09.022 Abstract （401）      PDF（pc） （471KB）（1086）       Knowledge map    Save Entomopathogenic nematodes (EPN) are symbiotic with bacteria and play a vital role as biological control agents against pests.The research of pathogenicity of EPN is fundamental for the efficient application of EPN,involving the interaction between nematodes and their symbiotic bacteria on insects.In this paper,we reviewed the pathogenic factors of the EPNs of Steinernema spp.and Heterorhabditis spp.and the interaction mechanism between nematodes and host insects.The review would provide reference for further theoretical research and application of EPN. Reference | Related Articles | Metrics | Comments（0） Select The Application Potential of Brevibacillus laterosporus in Agriculture LIU Fang, WU Hongqu, WEN Shaohua, FANG Wei, WANG Kaimei Chinese Journal of Biological Control    2023, 39 (1): 231-240.   DOI: 10.16409/j.cnki.2095-039x.2023.02.013 Abstract （394）      PDF（pc） （414KB）（928）       Knowledge map    Save Brevibacillus laterosporus shows broad spectra of bioactivities which make itself great importance of application in agriculture. In this review, the bioactivities of B. laterosporus in the biological control of plant disease and insect pests, plant-growth promoting and stress tolerance, the improvement of soil fertility and the microbial bioremediation of environment were summarized, and the problems existing in its large-scale application in agriculture were analyzed. The development trend and application potential in agriculture were further prospected. Reference | Related Articles | Metrics | Comments（0） Select Predatory Function of Harmonia axyridis and Propylea japonica Larvae to Young Larvae of Tuta absoluta YANG Guiqun, FAN Wei, ZHANG Qian, LI Mao, JIANG Zhengxiong, DUAN Pan, HU Changxiong, CHEN Guohua, ZHANG Xiaoming Chinese Journal of Biological Control    2022, 38 (4): 959-966.   DOI: 10.16409/j.cnki.2095-039x.2021.09.017 Abstract （377）      PDF（pc） （483KB）（1138）       Knowledge map    Save The control ability of Harmonia axyridis and Propylea japonica on Tuta absoluta was evaluated by investigating the predatory function and searching responses of the 4th instar larvae H.axyridis and P.japonica to the young larvae of T.absoluta (1stand 2nd instars) under laboratory conditions.The results showed that the predatory functional responses of the 4th instar larvae H.axyridis and P.japonica to the 1st and 2nd instar larvae of T.absoluta conformed to HollingⅡ model;the daily predation of the two ladybirds species was positively correlated with the prey density within a certain range,when the T.absoluta density was N→+∞,the maximum daily predation Namaxof H.axyridis and P.axyridis to the 1st instar T.absoluta larvae were 147.06 and 333.33,respectively,and to the 2nd instar T.absoluta larvae,140.85 and 149.25,respectively.The highest daily predation rate of H.axyridis reached 98%,significantly higher than that of P.axyridis.The searching efficiency of the two ladybirds species on T.absoluta decreased with the increase of prey density,which was higher in H.axyridis than in P.axyridis.The highest searching efficiency of H.axyridis was >0.90,while that of P.axyridis was<0.80.The predatory ability of H.axyridis to low instar larvae of T.absoluta was slightly stronger than that of P.axyridis,but both the ladybirds species afforded high predation and control of young larvae of T.absoluta.The two ladybirds species can be further studied as important biological control agents for control of T.absoluta. Reference | Related Articles | Metrics | Comments（0） Select Population Fitness of the Hoverfly, Episyrphus balteatus (De Geer) (Diptera:Syrphidae) Fed on Different Aphid Species JIANG Shanshan, LI Hui, HE Limei, WU Kongming Chinese Journal of Biological Control    2023, 39 (2): 254-263.   DOI: 10.16409/j.cnki.2095-039x.2022.03.012 Abstract （377）      PDF（pc） （485KB）（1528）       Knowledge map    Save Episyrphus balteatus (De Geer) is one of the most common predatory natural enemies in agricultural ecosystems. It has dual ecological service functions of pollination and biological control and plays an important role in improving crop yield and protecting biodiversity. However, the proper aphid species for artificial rearing of the predator is still unclear. Our study on the relationship between three aphid species, Aphis craccivora Koch, Myzus persicae Sulzer, and Megoura japonica Matsumura and fitness of E. balteatus showed that the developmental durations of larva (5.9 d) and pupa (6.0 d) with M. japonica were significantly shorter than those with the other preys, and the larval survival rate (80.0%) was the largest; the highest pupation rate (88.4%) and pupal emergence rate (100.0%) were attained with the prey A. craccivora, the lowest pupation rate (47.6%) and pupal emergence rate (49.6%) were recorded with M. persicae; With A. craccivora, M. persicae and M. japonica, the average generation period (T) of the predator was 30.26 d, 32.41 d and 32.34 d, respectively; the intrinsic rate of increases (r) was 0.1581, 0.0953 and 0.1424, respectively; and the finite rate of increase (λ) was 1.1713, 1.1002 and 1.1531, respectively. Flight ability tests showed that prey species had a significant effect on the flight velocity of adults, the highest velocity (0.29 m/s) was recorded with the prey M. japonica, in contrast to the lowest velocity (0.23 m/s) with M. persicae. This study indicates that M. japonica is a suitable aphid species for artificial rearing of E. balteatus, which is of theoretical and practical significance for large-scale rearing and utilization of the biocontrol and pollination ecological service functions of E. balteatus. Reference | Related Articles | Metrics | Comments（0） Select Identification of Two Common Larval Parasitic Wasps of Ectropis obliqua and Ectropis grisescens (Lepidoptera: Geometridae) ZHOU Xiaogui, Tang Pu, Wu Qiong, GUO Huawei, XIAO Qiang, CHEN Xuexin Chinese Journal of Biological Control    2023, 39 (1): 1-9.   DOI: 10.16409/j.cnki.2095-039x.2022.01.003 Abstract （369）      PDF（pc） （2716KB）（1198）       Knowledge map    Save Ectropis obliqua (Prout) and Ectropis grisescens Warren (Lepidopotera: Geometridae) are two of the most destructive chewing pests in China’s tea plantations. Two of the parasitic wasps, which previously reported as Apanteles spp. (Hymenoptera: Braconidae: Microgastrinae), can play important role in controlling Ectropis spp. during the larval stage in nature. In order to determine the species names and morphological differences of the two wasps, and to confirm whether these two species are common parasitic wasps of E. obliqua and E. grisescens, we identified the wasp specimens emerged from E. obliqua and E. grisescens that were collected from 22 locations in 8 provinces of China and reared in the laboratory, and compared the COI sequences of the voucher specimens with those of known species recorded in the BOLD database. The findings revealed that both parasitic wasps emerged from E. obliqua as well as E. grisescens during the larval stage. Based on morphological and molecular data, these two species of Apanteles spp. were identified as Protapanteles immunis (Haliday, 1834) and Parapanteles hyposidrae (Wilkinson, 1928). In conclusion, this study has confirmed the specific species names and morphological characteristics of the two wasps reported as Apanteles sp. in China more than 40 years ago, which are common larval parasitic wasps of E. obliqua and E. grisescens, and the P. immunis is recorded for the first time in China. These results provide a scientific basis for accurately identifying indigenous dominant parasitic wasps, which might help manage E. obliqua and E. grisescens. Reference | Related Articles | Metrics | Comments（0） Select Effects and Colonization of Beauveria bassiana in Maize under Stress of Exserohilum turcicum Infection SUI Li, LU Yang, CHI Ruikai, ZHAO Yu, ZHANG Zhengkun, LI Qiyun Chinese Journal of Biological Control    2023, 39 (4): 804-812.   DOI: 10.16409/j.cnki.2095-039x.2022.05.008 Abstract （358）      PDF（pc） （1371KB）（1406）       Knowledge map    Save This study aims to clarify the colonization ability of Beauveria bassiana in maize plants and its influence on disease resistance of host plants. B. bassiana-maize symbiosis was constructed by root drenching method and chlorophyll and nitrogen content, as well as plant height were measured to evaluate the growth promoting effect of B. bassiana colonization in maize. The resistance of B. bassiana colonization against Exserohilum turcicum infection was investigated by inoculation of the disease. The colonization and spread of B. bassiana endophytic in maize plants under E. turcicum infection stress was studied by colony counting and quantitative PCR. The results showed that the colonization of B. bassiana in maize reached 68.18%, positively promoted plant growth and significantly reduced the incidence of B. bassiana by 46.67% at 21 day after inoculation. B. bassiana could be 'recruited' from the rhizosphere soil when maize leaves were infected by E. turcicum, and B. bassiana could accumulate at leaves in turn to improve resistance against plant pathogen infection. This study has further clarified the biocontrol potential of entomogenous fungi against plant diseases as endophyte, and provides a theoretical basis for the study of colonization and induced resistance of entomogenous fungi in plant tissues. Reference | Related Articles | Metrics | Comments（0）