Most Downloaded

Published in last 1 year | In last 2 years| In last 3 years| All| Most Downloaded in Recent Month | Most Downloaded in Recent Year|

All

Please wait a minute...


For Selected: Download Citations EndNote Ris BibTeX Toggle Thumbnails

Select

Visual System and Opsin in Insect TANG Yanhong, BI Siyan, WANG Xiaodi, MA Dongfang, YANG Nianwan, GUO Jianyang, LÜ Zhichuang, LIU Wanxue Chinese Journal of Biological Control    2023, 39 (3): 718-730.   DOI: 10.16409/j.cnki.2095-039x.2023.05.004 Abstract （423）      PDF（pc） （1851KB）（11732）       Knowledge map    Save Insects have a well-developed and sensitive visual system, and opsin is a basic component of animal vision, and its structure largely determines the sensitivity of visual pigments to light of different wavelengths. The duplication and differentiation of opsin genes are the main mechanisms for the production of new light pigment sensitivity. Throughout the evolution of animals, many instances of opsin gene duplication and loss have occurred, forming the sensitivity of the various animal visual systems we observe today. In recent years, extensive and in-depth research has been carried out on visual proteins. This article describes the types of photoreceptors, the color vision system of insects, the classification of visual proteins, the phenomenon of co-expression of visual proteins, the duplication and deletion of visual protein genes, and the mechanism and research status of the physiological functions of visual proteins. Reference | Related Articles | Metrics | Comments（0）

Select

Research Progress on Ecological Regulation to Crop Pests using Agricultural Biodiversity YAN Xueying, WANG Hui, ZHANG Yanjun, ZHAO Jianning, YANG Dianlin, ZHANG Guilong Chinese Journal of Biological Control    2023, 39 (3): 710-717.   DOI: 10.16409/j.cnki.2095-039x.2023.05.003 Abstract （394）      PDF（pc） （393KB）（11298）       Knowledge map    Save At present, in the context of protecting biodiversity, maintaining the sustainable and healthy development of ecosystems and reducing the use of fertilizers and pesticides, using agricultural biodiversity to control pests has become one of the hotspots in domestic and foreign research. Agricultural biodiversity is important for maintaining the function of agricultural ecosystem and the control of crop pests are of great significance. This paper briefly describes the concept and current situation of agricultural biodiversity, and summarizes the scientific principles or theories of pest ecological regulation of biodiversity, starting from the protection of agricultural biodiversity by non-agricultural habitats and its influencing factors on agricultural biodiversity. The methods and specific applications of ecological regulation to crop pests using agricultural biodiversity are analyzed at the three levels of farmland, farmland boundary and farmland landscape pattern, and finally put forward the development direction of ecological regulation to crop pests using agricultural biodiversity, which provides knowledge, principles, methods and technical support for effective pest ecological regulation. Reference | Related Articles | Metrics | Comments（0）

Select

Mechanism of Chaetomium globosum in Promoting Growth and Preventing Disease and Advance of Its Application and Research FENG Chaohong, LI Lijuan, ZHANG Jiaojiao, WANG Junmei, SONG Yuli, LI Haohai, XU Fei Chinese Journal of Biological Control    2023, 39 (4): 961-969.   DOI: 10.16409/j.cnki.2095-039x.2023.02.043 Abstract （520）      PDF（pc） （432KB）（11283）       Knowledge map    Save Chaetomium globosum, one of common plant endophytic fungi, possesses potential biocontrol function against many plant diseases. This paper summarized the research advances of Chaetomium globosum on biocontrol effect, and its biocontrol mechanisms, which include antibiosis, growth-promoting effect, induction of plant resistance, niche and nutrient competition, and hyperparasitism. Chaetomium globosum exhibits great application potential on plant disease control and growth promotion, soil improvement, and straw degradation. Advances on the fermentation technology and genetic engineering were also illuminated. This study will provide theoretical references for the application research of Chaetomium globosum as biocontrol agent. Reference | Related Articles | Metrics | Comments（0）

Select

Analysis of Inhibitiory Active Substances of Penicillium bilaiae Strain 47M-1 Based on Non-Targeted Metabolomics ZHAO Xinbei, NI Yunxia, LIU Xintao, ZHAO Hui, YAN Wenqing, HE Bipo, LIU Hongyan Chinese Journal of Biological Control    2023, 39 (5): 1156-1171.   DOI: 10.16409/j.cnki.2095-039x.2023.02.055 Abstract （278）      PDF（pc） （1605KB）（9956）       Knowledge map    Save Strain 47M-1 of Penicillium bilaiae showed significant inhibitory effect on a variety of sesame pathogenic fungi. In order to analyze the inhibitiory active substances produced by this strain, liquid chromatography tandem mass spectrometry was used to detect the metabolites of strain cultured for 48 h, 96 h and 144 h. Then, classification of up-regulated differential metabolites in the HMDB database and enrichment of KEGG metabolic pathways were analyzed in order to identify the active metabolites. A total of 105 up-regulated differential metabolites were detected, which were mainly enriched in the superclasses of phenylpropanoids and polyketides. The enrichment analysis of the KEGG metabolic pathways showed that the up-regulated differential metabolites were mainly enriched in metabolic pathways, biosynthesis of cofactors, purine metabolism, biosynthesis of amino acids and ABC transporters. The inhibitiory activities of some up-regulated differential metabolites were verified using medicated plate method. These compounds naringenin, protocatechuic acid, D-(-)-quinic acid, hesperetin, 3-(3-Hydroxyphenyl) propanoic acid, 3, 4-dihydroxyhydrocinnamic acid, 5, 7-dihydroxy-2-(4-hydroxyphenyl)-3, 4-dihydro-2H-1-benzopyran-4-one, rosmarinic acid, bergapten and eriodictyol all showed excellent inhibition activities against sesame pathogenic fungi. In this study, the major groups and key metabolic pathways of potential inhibition metabolites produced by strain 47M-1 were identified, and the inhibitiory activity of some up-regulated differential metabolites was verified. This study will provide data support for the further development and utilization of strain 47M-1 and the research and development of microbial pesticide. Reference | Related Articles | Metrics | Comments（0）

Select

Optimization of Genetic Manipulation System for Streptomyces pratensis S10 HU Lifang, CHENG Jing, JIA Ruimin, WANG Yang Chinese Journal of Biological Control    2023, 39 (3): 624-635.   DOI: 10.16409/j.cnki.2095-039x.2023.02.029 Abstract （296）      PDF（pc） （3493KB）（8043）       Knowledge map    Save Streptomyces pratensis S10 was isolated from tomato leaf mould, exhibiting a potential application in biological control. It is of great significance to establish an efficient and stable genetic manipulation system to carry out gene-directed modification, improve the level of gene expression, and further explore the biocontrol molecular mechanism of S. pratensis S10. In this study, a genetic transformation system based on intergeneric conjugation was optimized in S. pratensis S10, the temperature sensitive vector pKC1139 was used as the plasmid, Escherichia coli ET12567 (pUZ8002) used as the donor strain, and S. pratensis S10 was the recipient. The results show that the optimum conjugation transformation with a conjugation efficiency of 8.3×10-7was developed under following conditions: Gauze’s medium No.1 as S. pratensis S10 and E. coli junction transfer medium, spores were heat shocked at 50 ℃ for 10 min, 1:100 for donor-recipient ratio, 25 μg/mL apramycin coverage 16 h post transconjugation, and adding MgCl2 till final concentration of 15 mmol/L. Subsequently, based on the above optimized genetic manipulation system, a double-crossover mutant of a regulating gene was successfully obtained. A stable and efficient genetic manipulation system for S. pratensis S10 was developed, which lays a solid foundation for the further constructing high-yield strain and studying the active substance biosynthesis mechanism. Reference | Related Articles | Metrics | Comments（0）

Select

Review on Research and Development of Botanical Pesticides ZHANG Xing, MA Zhiqing, FENG Juntao, WU Hua, HAN Lirong journal1    2015, 31 (5): 685-698.   DOI: 10.16409/j.cnki.2095-039x.2015.05.009 Abstract （1338）      PDF（pc） （599KB）（6980）       Knowledge map    Save Botanical pesticides have aroused the researchers' great interest all over the world. The article will give a concise elucidation to botanical pesticides from several aspects including the research history, current situation, industrialization efforts, application technique emphasis, special activities, biosynthesis technique, environment pattern and its mechanism, the effects of comprehensive use the residues from botanical pesticides and “pesticides-fertilizer unification” to novel plant protection theory and practice. The article present the problems appeared in fields of R & D, application, marketing promotion and management concerning botanical pesticides, and give the relevant countermeasures. And also, the article discussed the development trend and emphasis of botanical pesticides. Reference | Related Articles | Metrics | Comments（0） Cited: Baidu(29)

Select

Antifungal Mechanism Analysis and Verification of Bacillus velezensis Ba-0321 Based on Whole Genome Sequencing LI Xiaojie, QIU Rui, LIU Chang, YAO Chenxiao, BAI Jingke, CHEN Yuguo, LI Shujun Chinese Journal of Biological Control    2023, 39 (4): 885-894.   DOI: 10.16409/j.cnki.2095-039x.2023.02.035 Abstract （293）      PDF（pc） （2615KB）（6240）       Knowledge map    Save Bacillus velezensis Ba-0321, isolated from the rhizosphere soil of healthy tobacco plants, is a plant growth promoting rhizobacteria with strong antifungal activity. It has a good potential in biocontrol application. In the study, the inhibitory effects of strain Ba-0321 sterile filtrate on Fusarium oxysporum and Phytophthora nicotianae were determined by methods of co-culture. The whole genome of strain Ba-0321 was sequenced using the second-generation Illumina and the third-generation Nanopore platform, then analyzed for genome assembly, gene functional annotation, prediction of secondary metabolite synthetic gene clusters. The results showed that the sterile filtrate of strain Ba-0321 has inhibitory effects on both F. oxysporum and P. nicotianae. The culture medium containing 10% sterile filtrate has a higher inhibitory rate on the mycelial growth of the two pathogens, reaching 57.38% and 34.30%, respectively. The results of whole genome sequencing showed that the genome size of strain Ba-0321 was 4099109 bp, including 3897 coding genes, and the sequencing data are available in the GenBank database (accession No. CP101904). There were a large number of genes encoding enzymes, terpene, polyketone metabolic pathways, and genes involved in defense mechanisms have been annotated in the GO, COG and KEGG databases. Using anti-SMASH software, 13 secondary metabolite synthesis gene clusters were predicted encoding antibacterial substances such as Surfactin、Fengycin、difficidin、Bacillaene、Bacillibactin、Macrolactin H、Bacilysin. The existence of biosynthetic gene cluster for antibacterial substances and defence machainsm related genes in Ba-0321 strain genome were verified by PCR amplification and sequencing. The enzyme activity test results showed that the strain has resistant enzyme activities such as protease, chitinase, and cellulase.. This study provides a basis for analyzing the antimicrobial mechanism of strain Ba-0321 and mining antifungal related gene resources at the genomic level, which is of a great significance for research and application of the Ba-0321 strain in future. Reference | Related Articles | Metrics | Comments（0）

Select

Dissection on the Agronomical Functions of Photosynthetic Bacteria SU Pin, ZHANG Deyong, ZHANG Zhuo, CHEN Ang, CHENG Ju'e, ZENG Jun, TAN Shiyong, DAI Jianping, LIU Yong Chinese Journal of Biological Control    2021, 37 (1): 30-37.   DOI: 10.16409/j.cnki.2095-039x.2021.02.012 Abstract （549）      PDF（pc） （382KB）（6229）       Knowledge map    Save Photosynthetic bacteria (PSB) are summarized as the group of microbes capable of carrying out photosynthesis with the employment of Bacteriochlorophyll. Members of PSB are regarded as important components in microbiome from plant surface and soil. Attributed to their extraordinary metabolism versatility, photosynthetic bacteria often play significant roles in processes like plant nutrient uptake, growth and development, as well as stress resistance and establish close relationships with plants and other microorganisms in agroecosystem. This confers PSB a reputation as plant beneficial microorganism. In this article, we mainly focus on the PSB-produced effects on the plant nutrition, growth promotion, stress resistance and disease management. The biological activities carried out by PSB, e.g., nitrogen fixation, phosphate solubilization, heavy metal immobilization, phytohormone and plant growth regulator production, antimicrobial compound synthesis, plant systemic resistance induction, are illustrated. Those activities are recognized as the main resorts to maintain the plant and soil health. In conclusion, as a group of microbes with properties including metabolism versatility, wide distribution, flexibly adaptive to environment, and intimately interactive with plants, photosynthetic bacteria can be exploited as a valuable resource for development of novel multi-functional bio-fertilizer and bio-control agent. Reference | Related Articles | Metrics | Comments（0）

Select

Research and Application of Nanoparticle-mediated RNAi Technology in Pest Control WANG Xiaodi, JI Shunxia, SHEN Xiaona, LIU Wanxue, WAN Fanghao, ZHANG Guifen, Lü Zhichuang Chinese Journal of Biological Control    2021, 37 (6): 1298-1312.   DOI: 10.16409/j.cnki.2095-039x.2021.01.020 Abstract （433）      PDF（pc） （2140KB）（6000）       Knowledge map    Save Each year, the epidemic and outbreak of major diseases and pests will have a major impact on agriculture, resulting in serious economic losses, with the application of chemical reagents its resistance has gradually increased, therefore, it is of great significance to explore new green, safe and efficient pest control technology. RNAi, as a tool to study gene function, has shown great potential in developing new pest control strategies. However, the efficiency of RNAi is limited by the degradation effect of nuclease. In order to improve the effective delivery of dsRNA, nanoparticle-mediated technology was developed and widely used in pest control. At the same time, the efficient synthesis of targeted insect dsRNA based on engineered bacteria provides feasibility and technical support for field promotion. This paper mainly summarized the research and application of RNAi technology and nanoparticle-mediated technology in pest control. Reference | Related Articles | Metrics | Comments（0）

Select

Research Progress of Plant Biostimulants XIE Shangqiang, WANG Wenxia, ZHANG Fuyun, YIN Heng journal1    2019, 35 (3): 487-496.   DOI: 10.16409/j.cnki.2095-039x.2019.03.017 Abstract （755）      PDF（pc） （733KB）（5879）       Knowledge map    Save The plant biostimulants are diverse substances or microorganisms which include humic acid, chitin and its derivatives, seaweed extracts, etc. applied to enhance plant growth, development, tolerance to abiotic stresses and crop quality traits regardless of its nutrient content. The emergence of biostimulants has provided new ideas and solutions for the safety of agricultural products, and they have been widely used in agricultural production. They also have become a powerful tool for improving the efficiency of fertilizers and pesticides. The purpose of this review is to better under-standing of the concept of plant biostimulants including main categories and regulatory mechanisms in plants. Moreover, the characteristics of these biostimulants and its effects on plant growth, development, tolerance to abiotic stress, plant nutrition and crop quality traits are thoroughly described. In addition, this paper also briefly described the application prospects of plant biostimulants in agricultural production, opportunities and future challenges. Reference | Related Articles | Metrics | Comments（0）

Select

The Development of Biological Pesticides Based on RNA Interference GAO Liwen, CHEN Shiguo, ZHANG Yu, QIANG Sheng Chinese Journal of Biological Control    2022, 38 (3): 700-715.   DOI: 10.16409/j.cnki.2095-039x.2021.11.015 Abstract （782）      PDF（pc） （511KB）（5782）       Knowledge map    Save RNA interference, also known as post transcriptional gene silencing, is an emerging new genetic engineering technique that can effectively silence or inhibits the expression of target genes. Biopesticides based on RNA interference are considered as a revolutionary technique in the field of plant protection in the future, which will completely change the ideas and strategies of controlling agricultural diseases, insects, weeds and other pests. Here, the basic principle and development process of RNA interference were briefly reviewed. The current status of research and application of RNAi biopesticides was summarized. The opportunities and challenges to develop RNAi biopesticides as well as its development prospect were deeply analyzed. This review serves to provide some valuable references for the development of RNAi biopesticides in China. Reference | Related Articles | Metrics | Comments（0）

Select

Comparative Transcriptomic Profiles Revealed JA- and SA- Regulated Soft Rot Disease Resistance by Bacillus velezensis BCP6 in Amorphophallus konjac ZHANG Meng, CHEN Sen, TANG Dengguo, HUANG Yan, LONG Zhijian, WANG Boya, HU Shanglian, CAO Ying Chinese Journal of Biological Control    2024, 40 (6): 1375-1385.   DOI: 10.16409/j.cnki.2095-039x.2024.02.060 Abstract （126）      PDF（pc） （4362KB）（5263）       Knowledge map    Save The application of Bacillus velezensis has been proven to significantly enhance the resistance to soft rot in Amorphophallus konjac, yet its precise biological control mechanisms remain unclear. This study explored the effects of B. velezensis BCP6 on the defense pathways of A. konjac in response to P. aroidearum MY11 infection through comparative transcriptome analysis. Differential expression analysis, gene set enrichment analysis (GSEA) and gene set variation analysis (GSVA) revealed that BCP6 application upregulated pathways related to jasmonic acid (JA), reactive oxygen species, and cell wall synthesis, thereby activating the basic defense system of A. konjac. This immune activation facilitated the rapid initiation of defense responses when subjected to subsequent P. aroidearum infection by enhancing the JA-mediated induced systemic resistance (ISR) and SA-dependent systemic acquired resistance (SAR) signaling pathways. Simultaneously, a significant increase in the expression of genes involved in cell wall formation and pathogenesis-related proteins was observed, effectively enhancing resistance to soft rot. Meanwhile, exogenous JA treatment was found to directly stimulate the expression of cell wall-related genes, namely CESA2 and CSI1, while SA significantly induced the expression of SA-dependent defense genes TLP and E13C. The results provided a theoretical basis for biological control and disease-resistant breeding of konjac. Reference | Related Articles | Metrics | Comments（0）

Select

Advances in Research on Endophytic Trichoderma in Plants LI Mei, TIAN Ying, JIANG Xiliang Chinese Journal Of Biological Control    2020, 36 (2): 155-162.   DOI: 10.16409/j.cnki.2095-039x.2020.02.001 Abstract （556）      PDF（pc） （394KB）（5029）       Knowledge map    Save Endophytes are widely present in plants. Endophytic Trichoderma are common endophytic fungi, some of them can promote plant growth, improve plant stress and disease resistance by antagonism, mycoparasitism, producing secondary metabolites, and inducing system resistance in plants. Endophytic Trichoderma received widespread attention in recent years as important biocontrol microbial resource. Recent research advances on the species, distribution and function of endophytic Trichoderma, and the molecular mechanism of interaction between endophytic Trichoderma and their host plants are reviewed in this paper for providing a basis on the utilization of Endophytic Trichoderma as biocontrol factors. Reference | Related Articles | Metrics | Comments（0）

Select

Statue and Analysis of Overseas Natural Enemies Merchandise XU Xue-nong, WANG En-dong    Abstract （648）      PDF（pc） （183KB）（5028）       Knowledge map    Save Related Articles | Metrics | Comments（0） Cited: Baidu(23)

Select

Research Progress on Biology, Ecology and Biological Control of Cheilomenes sexmaculata (Coleoptera: Coccinellidae) TANG Liangde, ZANG Liansheng Chinese Journal of Biological Control    2023, 39 (3): 697-709.   DOI: 10.16409/j.cnki.2095-039x.2023.05.002 Abstract （435）      PDF（pc） （467KB）（5010）       Knowledge map    Save The ladybeetle Cheilomenes sexmaculata (Fabricius) (Coleoptea: Coccinellidae) is one of the dominant natural enemies of various insect pests in agroforestry ecosystem due to wide distribution, common occurrence in field, high reproductive rate, wide feeding range, excellent predation ability and strong adaptability, thus there is a bright development prospect to develop this predator. In this article we reviewed the research progress on C. sexmaculata in recent 40 years, mainly focusing on the taxonomy, biological characteristics, biological control, artificial rearing, and insecticide impacts. In addition, the prospects for the future studies and application of C. sexmaculata were also discussed. Reference | Related Articles | Metrics | Comments（0）

Select

Inhibition Effect and Transcriptome Analysis of Isooctyl alcohol on Botrytis cinerea DUAN Tiankun, WANG Yan, YUAN Jiaqi, SU Yaxin, SHI Luxin, WANG Lin, SU Jian, WANG Meiqin, WANG Chunwei Chinese Journal of Biological Control    2023, 39 (6): 1434-1445.   DOI: 10.16409/j.cnki.2095-039x.2023.02.068 Abstract （175）      PDF（pc） （2850KB）（4717）       Knowledge map    Save To reveal the inhibitory mechanism of isooctyl alcohol to Botrytis cinerea, the inhibition effect of isooctyl alcohol on B. cinerea was determined using two-sealed-base-plate method. Mycelial morphology treated with isooctyl alcohol was observed by scanning electron microscopy. The influence of isooctyl alcohol on the extracellular conductivity and the ergosterol synthesis were measured. Response of B. cinerea to isooctyl alcohol was analyzed by RNA sequencing (RNA-Seq). The results showed that the inhibition rate of isooctyl alcohol to mycelia was 67.92%. After treatment with isooctyl alcohol, lots of mycelia were deformed and distorted with the increasing of membrane permeability. At 4, 5 and 6 days after isooctyl alcohol treatment, the ergosterol contents in cell membrane decreased to 44.35%, 38.82% and 42.43%, and the protein concentration decreased to 65.70%, 77.93% and 58.57%, respectively. A total of 577 differentially expressed genes (DEGs), including 312 up-regulated genes and 265 down-regulated genes, were obtained by RNA-Seq. Based on functional analysis of clusters of orthologous groups (COG), gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG), DEGs involved in membrane integral components, ergosterol biosynthesis pathway, amino acid metabolism and ribosome biogenesis pathway were significantly down-regulated, indicating that isooctyl alcohol might affect ergosterol content, energy production and ribosome structure, and further play inhibit role in the growth of B. cinerea. Reference | Related Articles | Metrics | Comments（0）

Select

Advances on Biological Control of Plant-parasitic Nematodes JIN Na, LIU Qian, JIAN Heng journal1    2015, 31 (5): 789-800.   DOI: 10.16409/j.cnki.2095-039x.2015.05.019 Abstract （480）      PDF（pc） （459KB）（4669）       Knowledge map    Save Fungi, bacteria and actinomycetes have recognized as effective biocontrol agents to plant-parasitic nematodes. In this review, we have summarized the advances on screening, application and commercial formulations of biological agents during the past five years, and have proposed some suggestions for future researches. Reference | Related Articles | Metrics | Comments（0） Cited: Baidu(9)

Select

Screening of Biocontrol Streptomyces against Fusarium Wilt of Watermelon and Its Study on Control Mechanism TIAN Lin, CHEN Jing, WANG Yang Chinese Journal of Biological Control    2023, 39 (3): 657-666.   DOI: 10.16409/j.cnki.2095-039x.2023.02.032 Abstract （220）      PDF（pc） （2505KB）（4455）       Knowledge map    Save In order to prevent and control watermelon Fusarium wilt safely and efficiently, in this study, seven Streptomyces with good antagonistic effect against Fusarium oxysporum f. sp. niveum (FON) were selected using plate confrontation method. The inhibitory effect of Streptomyces fermentation filtrate and bacterial crude extract on the growth of FON was investigated. Among these seven Streptomyces, Streptomyces tunisialbus YC2-17 and S. eurocidicus YC5-15 showed strong inhibitory effect on FON,with inhibitory rates of 56.21% and 54.72%. In addition, the plant growth-promoting experiments on watermelon seedlings were carried out. The results showed that the two Streptomyces had significant promoting effects on the growth of watermelon seedlings, and YC2-17 had better promoting effect. After YC2-17 treatment, the plant height, root length, fresh weight and dry weight of watermelon seedlings increased by 52.54%, 27.65%, 109.57% and 100.00%. The result of the potted plant experiments demonstrated that the control effects of YC2-17 and YC5-15 fermentation solution on watermelon Fusarium wilt were 56.82% and 50.45%, respectively. The treatment of ethyl acetate phase crude extract of YC2-17 fermentation filtrate could inhibit the formation and germination of FON spores, change the morphology of spores and mycelium. After treatment, the external morphology of hyphae and spores was abnormal, the trunk were flat, and the contents were exuded. Reference | Related Articles | Metrics | Comments（0）

Select

Infection and Application of Beauveria spp. JI Xiang-yun;YANG Chang-ju    Abstract （586）      PDF（pc） （173KB）（4192）       Knowledge map    Save Related Articles | Metrics | Comments（0）

Select

Isolation, Identification and Evaluation of Biocontrol Bacteria against Cucumber Target Spot ZHANG Xiaoyun, Cong Rong, GUO Qinggang, LU Xiuyun, SU Zhenhe, CHEN Xiuye, LI Shezeng, MA Ping Chinese Journal of Biological Control    2023, 39 (1): 194-203.   DOI: 10.16409/j.cnki.2095-039x.2023.02.002 Abstract （351）      PDF（pc） （1178KB）（4081）       Knowledge map    Save Cucumber target spot caused by Corynespora cassiicola is a serious disease of cucumber in recent years. Bacterial strain HMB28521 with potential biocontrol ability for cucumber target spot was obtained by dual culture method and pot experiment in greenhouse. Strain HMB28521 was identified as Bacillus velezensis based on morphological, physiological, biochemical characteristics, 16S rDNA sequence analysis, as well as phylogenetic tree inferred from the alignment sequence of gyrA, gyrB, rpoB and rpoC. Lipopeptides iturin, fengycin, and surfactin were isolated, purified and identified from strain HMB28521 by fast protein liquid chromatography (FPLC) and UPLC-Triple TOF-MS/MS. The iturin and fengycin showed strong inhibitory abilities against the growth of C. cassiicola, as well as causing abnormal hyphal growth in vitro. This study provided new resources for the biological control of cucumber target leaf spot and a theoretical basis for further clarifying the action mechanism of the biocontrol bacteria. Reference | Related Articles | Metrics | Comments（0）