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Published in last 1 year |  In last 2 years |  In last 3 years |  All Please wait a minute... For Selected: Download Citations EndNote Ris BibTeX Toggle Thumbnails Select Research Progress on Green and Sustainable Management Technologies for Spodoptera frugiperda WEI Lei, NIE Ying, LI Yongjun Chinese Journal of Biological Control    2025, 41 (1): 207-219.   DOI: 10.16409/j.cnki.2095-039x.2024.07.004 Abstract （457）      PDF（pc） （530KB）（3527）       Knowledge map    Save The fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), is a major worldwide disastrous pest. Since its invasion into China in 2019, the implementation of emergency chemical control measures yielded positive outcomes. However, the occurrence of S. frugiperda in China has now become common and year-round, necessitating a long-term approach for its management. Throughout the history of global pest control, reliance on chemical agents has invariably led to the development of pest resistance. Incidences of pesticide resistance in S. frugiperda have been reported in certain regions of China. Therefore, to mitigate the adverse effects of chemical pesticides and the risk of pesticide resistance, China's management strategies must transition towards green, efficient, precise, and sustainable approaches. On the basis of the regional control strategy and unified management strategy, the Ministry of Agriculture and Rural Affairs has advocated a management framework emphasizing biological control as the focal point, ecological and agricultural control as the foundation, and chemical control as the last resort. This article elaborates on recent technological advancements both domestically and internationally in biological control, ecological and agricultural control methods, with an emphasis on biological control. Additionally, it proposes future research directions and ideas to overcome existing challenges, aiming to provide guidance for the development of green management technologies in China. Reference | Related Articles | Metrics | Comments（0） Select Screening of Bacillus velezensis HMB28023 for Controlling Grape Gray Mold and Identification of Its Antifungal Active Compounds ZHANG Xiaoyun, WANG Xuemei, CONG Rong, CHEN Xiuye, LU Xiuyun, LI Shezeng, GUO Qinggang, MA Ping Chinese Journal of Biological Control    2025, 41 (1): 122-131.   DOI: 10.16409/j.cnki.2095-039x.2025.02.005 Abstract （376）      PDF（pc） （1041KB）（763）       Knowledge map    Save Grape gray mold caused by Botrytis cinerea which significantly reduces the yield and quality of grape, but there is a lack of safe and effective microbial fungicides for its control. In this study, the antagonistic activity of 27 bacteria with high antagonistic activity against B. cinerea derived from tomato, was determined by the confrontation culture method. It was found that the inhibition zone and inhibition rate of the same bacterial strain against B. cinerea derived from tomato and grape exhibited variable changes. A bacterial strain HMB28023, was further obtained based on detached leaves method, potting test and field test, which field control efficacy on grape gray mold reached 74.3%. Strain HMB28023 was identified as Bacillus velezensis through morphological characteristics, 16S rDNA as well as phylogenetic tree constructed by using multiple gene sequences of gyrA, gyrB, rpoB and rpoC. The results of detached leaves test indicated that the fermentation broth, cell free supernatant and bacterial suspension of strain HMB28023 all exhibited high biocontrol efficacy against grape gray mold, reaching 81.9%, 75.1% and 64.8%, respectively. The lipopeptide produced by strain HMB28023 was isolated through fast protein liquid chromatography (FPLC) and identified by UPLC-Triple TOF-MS/MS. The results showed that the lipopeptide comprised iturin A (C14—C15), fengycin (fengycin A (C14—C17) and fengycin B (C16—C17)), and surfactin (C12—C16). Both iturin A and fengycin showed strong inhibitory activities against the growth of B. cinerea, and also caused abnormal hyphal growth in vitro. Reference | Related Articles | Metrics | Comments（0） Select Identification of Bacteria strain Sneb2010 and Its Control Effect on Melon Fusarium Wilt WU Ji, ZHU Xiaofeng, WANG Yuanyuan, LIU Xiaoyu, ZHAO Di, YANG Ning, DUAN Yuxi, FAN Haiyan, CHEN Lijie Chinese Journal of Biological Control    2024, 40 (6): 1331-1346.   DOI: 10.16409/j.cnki.2095-039x.2024.02.063 Abstract （375）      PDF（pc） （5504KB）（1025）       Knowledge map    Save Melon Fusarium wilt is a serious soil-borne disease caused by Fusarium oxyporum f. sp. melonis. In this study, the bacteria strain Sneb2010 with significant inhibitory effect on F. oxyporum f. sp. melonis was selected from 688 strains of bacteria by the dual culture technique, and the inhibition rate was 78.45%. The bacterium Sneb2010 was identified as Bacillus velezensis by morphological, physiological and biochemical characteristics combined with 16S rDNA and gyrA sequence analysis. The mycelium growth and spore germination of F. oxyporum f. sp. melonis were significantly inhibited by strain Sneb2010. After Sneb2010 treatment, the mycelia of F. oxyporum f. sp. melonis in the inhibited area showed abnormal phenomena, such as wizened, wrinkled, irregular protrusion and fracture. Strain Sneb2010 could promote the germination of melon seeds and the growth of seedlins. It was found that the strain Sneb2010 could produce protease and had the ability of biofilm forming. In addition, strain Sneb2010 showed strong inhibitory effects on a variety of pathogenic fungi. The results of pot experiment showed that, the control effect of Sneb2010 on melon Fusarium wilt was 64.29%, the number of pathogens decreased by 47%. In conclusion, B. velezensis Sneb2010 could effectively control melon Fusarium wilt, and provide a new potential biocontrol resource for the biological control of melon Fusarium wilt. Reference | Related Articles | Metrics | Comments（0） Select Effects of Host Plant and Temperature on the Development and Reproduction of Tetranychus truncatus Ehara Tuerxun·Ahemaiti, LIU Shuai, Aerziguli·Rouzi, WANG Bing, HA Ziyi, JIA Zunzun, DING Xinhua, FU Kaiyun, LI Kemei, GUO Wenchao Chinese Journal of Biological Control    2024, 40 (6): 1228-1236.   DOI: 10.16409/j.cnki.2095-039x.2024.07.005 Abstract （331）      PDF（pc） （475KB）（581）       Knowledge map    Save Tetranychus truncatus Ehara has a wide distribution, heterogeneous diet, fast reproduction rate, strong drug resistance and short-term outbreaks, making it an important pest mite that is seriously harmful and difficult to control, and the mite can also be used as a high-quality natural feed for some natural enemies insects. In this study, the effects of different host plants and temperature on the growth, development and reproduction of T. truncatus were studied. The results showed that the generation development of T. truncatus could be completed successfully at 25 ℃, 28 ℃, 31 ℃ and 34 ℃. At 28 ℃, the population doubling time (0.15) and Innate capacity for incease (1.73) were the shortest, the Net reproductive rate (80.26) and the Finite rate of increase (5.64) were higher than the other three temperatures, and the spawning period of P. sericulata was basically consistent with that at 25 ℃. But the spawning yield was higher than 25 ℃ continuously. After feeding on 8 host plants, there were significant differences in the life span of each mite state and the early oviposition stage, and the mite could survive and reproduce on 4 host plants, namely green bean, corn, eggplant and pepper, and the reproductive population showed an increasing trend, in which the number of oviposition was in the order of green beanm, corn, eggplant, pepper, peanut, tomato, cotton. The oviposition period of T. truncatus prunus on eggplant and green bean was basically the same, with the longest oviposition period reaching 28 d. The oviposition period of T. truncatus prunus on pepper was the shortest, with the longest oviposition period only reaching 21 d. In conclusion, the most suitable temperature and host plant for the growth and development of Tetramethylus prunus were 28 ℃ and green bean, respectively. In conclusion, the most suitable temperature and host plant for the growth and development of T. truncatus frustum were 28 ℃ and green bean, respectively. The aim of this study was to screen out the rather host and temperature for indoor breeding of T. truncatus laminae, so as to provide a stable feed source for the breeding of natural enemy insects, and also provide a theoretical basis for the comprehensive management of T. truncatus laminae. Reference | Related Articles | Metrics | Comments（0） Select Preparation of Wettable Powder Formulation of Trichoderma harzianum M-17 Chlamydospores and Its Field Control Effect on Potato Dry Rot WANG Xigang, GUO Chengjin, JIAO Yang, ZHAO Pei, TIAN Jing, ZHANG Lirong, SHEN Ruiqing Chinese Journal of Biological Control    2024, 40 (6): 1319-1330.   DOI: 10.16409/j.cnki.2095-039x.2024.02.061 Abstract （326）      PDF（pc） （576KB）（1563）       Knowledge map    Save To development an efficient biocontrol antimicrobial agent of potato dry rot and prolong the shelf of the product, this study utilized Trichoderma harzianum M-17 as the primary research object. Single factor selection and formulation optimization were used to obtain the wettable powder (WP). The optimal formulation of WP was chlamydospore powder (20%), attapulgite (68%) as carrier, sodium dodecyl sulfonate (4%) as wetting agent, sodium carboxylcellulose (7%) as dispersion, and ascorbic acid (1%) as UV protective agent. The chlamydospore content of this WP was 3.1×108 CFU/g, the wetting time was 56.5 s, the spore suspension rate was 82.27%, pH 6.79, the moisture content was 2.26%, the ratio transiting standard test sieve with 74 µm was 98%. All indicators meet the national standards. The results of field experiments showed that T. harzianum M-17 chlamydospore wettable powder could promote the growth of potato plants and indicated a good control effect on potato dry rot, with a control efficacy of 70.32% and a yield increase rate of 19.03%. This study lays a technical foundation for the development of T. harzianum M-17 chlamydospore wettable powder into a microbial fungicide for the control of potato soil-borne diseases. Reference | Related Articles | Metrics | Comments（0） Select Predation of Aphidoletes aphidimyza on Megoura crassicauda WU Huizi, HUANG Chunyang, WEN Mingxia, ZHANG Weiye, YU Xiaofei, YANG Maofa, ZHANG Feng Chinese Journal of Biological Control    2024, 40 (6): 1221-1227.   DOI: 10.16409/j.cnki.2095-039x.2024.03.016 Abstract （323）      PDF（pc） （406KB）（962）       Knowledge map    Save To determine the predation capacity of Aphidoletes aphidimyza to Megoura crassicauda, the functional responses of A. aphidimyza to M. crassicauda at different stages, the prey selection of A. aphidimyza on M. crassicauda at different stages and the interference effect of the density of A. aphidimyza on its predation capacity were studied in the laboratory. The results showed that the functional response of A. aphidimyza to each developmental stage of M. crassicauda was all fitted to the Holling II model. The predation capability of A. aphidimyza on young nymphs aged 1―2 of M. crassicauda was the strongest with a value of 16.23, followed by the 3rd instar nymph and the 4th instar nymph, with a value of 12.05 and 10.55, respectively. The daily maximum predation amount of A. aphidimyza on 1st―2nd instar nymph, 3rd instar nymph and 4th instar nymph of M. crassicauda were 29.24, 24.88, and 22.57. A. aphidimyza has a strong preference for the 1st―2nd instar nymph of M. crassicauda, with a selection coefficient (Q) of 1.67, 1.42, and 1.31, respectively. The total predation amount of A. aphidimyza on M. crassicauda increased with the density of A. aphidimyza, but the predation amount per predation number and predation rate decreased with the density of A. aphidimyza. The relationship between the rate and the density of A. aphidimyza were in line with the model E1＝0.2279p-0.519, E2＝0.2093p-0.550 and E3＝0.2022p-0.527, respectively. The results showed that A. aphidimyza had strong predation capability in each of the different developmental stages of M. crassicauda. This provides a theoretical foundation and data support for the pest control of M. crassicauda by A. aphidimyza. Reference | Related Articles | Metrics | Comments（0） Select Investigation and Identification of Parasitic Natural Enemy Tachinid Resources of Gynaephora qinghaiensis RUAN Xiaoyu, WANG Shasha, LAI Youpeng Chinese Journal of Biological Control    2025, 41 (2): 269-275.   DOI: 10.16409/j.cnki.2095-039x.2024.07.006 Abstract （310）      PDF（pc） （453KB）（598）       Knowledge map    Save In order to clarify the species and parasitic morphology of the parasitic natural enemy insect-parasitoid flies of the grassland caterpillars, three species of parasitoid flies hatched from the grassland caterpillar were identified in this study by morphological classification and molecular sequencing: Gymnophryxe inconspicua (Villeneuve, 1924), Exorista laruarum (Linnaeus, 1758) and Chetogena gynmephorae Chao et Shi, 1987. Among them, the dominant species of parasitic natural enemies of grassland caterpillars in this area are Gymnophryxe inconspicua and Exonista laruarum. Through the survey and statistics of the parasitism rate of natural enemies in grassland caterpillars, the parasitism rate of parasitoids in grassland caterpillars was found to be increasing and then decreasing, and the parasitoid flies mainly parasitized the 6th and 7th instar larvae of grassland caterpillars from July to August. In this experiment, indoor dissection revealed that a single prairie caterpillar hosted from 2 to 5 parasitoid larvae. The purpose of this study is to lay a foundation for the biological control technology of grassland caterpillars, and also to provide a theoretical basis for the parasitism mode, parasitism period and parasitism state of parasitic natural enemy insects, which is of great significance for the protection of the ecological environment of grassland in alpine rangeland of the Qinghai-Tibet Plateau. Reference | Related Articles | Metrics | Comments（0） Select Screening of Endophytic Fungi of Andrographis paniculata Resistant to Ralstonia solanacearum Infecting Peanuts and Study of Their Inhibitory Components HUANG Jin, HU Jinwen, DING Mei, YANG Fan, LUO Wenrong, DU Qin Chinese Journal of Biological Control    2025, 41 (2): 396-410.   DOI: 10.16409/j.cnki.2095-039x.2025.02.028 Abstract （306）      PDF（pc） （5856KB）（1335）       Knowledge map    Save To identify potential biocontrol strains against peanut bacterial wilt caused by Ralstonia solanacearum, we screened endophytic fungi of Andrographis paniculata leaves using the Oxford cup inhibition method. A strain with strong inhibitory activity against R. solanacearum was isolated and identified as Colletotrichum sp. CXL-11 based on morphological characteristics and 16S rDNA phylogenetic analysis. We further investigated its antibacterial mechanism through co-culture experiments and analyzed the antifungal components in its fermentation extract using LC-MS metabolomics. The bioactivity of selected metabolites against R. solanacearum was also validated. The results showed that the fermentation extract of CXL-11 produced an inhibition zone of 19.08 mm against R. solanacearum, with a minimum inhibitory concentration (MIC) of 0.625 mg/mL. The extract disrupted the membrane integrity of R. solanacearum, causing surface shrinkage and deformation of bacterial cells, and induced oxidative stress through the accumulation of reactive oxygen species. Metabolomic analysis revealed that the primary antifungal components were polyketides and coumarins, including isogentisin, bergapten, scopoletin, scopolin, and esculetin, all of which exhibited inhibitory effects against R. solanacearum. The biosynthesis pathway of cofactors was identified as a key mechanism underlying the antibacterial activity of CXL-11. In conclusion, the endophytic fungus Colletotrichum sp. CXL-11 demonstrates significant potential as a biocontrol agent for peanut bacterial wilt. This study provides theoretical support for the development of natural antimicrobial agents and highlights the promising application of CXL-11 in sustainable disease management. Reference | Related Articles | Metrics | Comments（0） Select Isolation and Identification of Antagonistic Bacterial Strain FCR-Y1 against Wheat Crown Rot and Its Effect on Disease Control and Growth Promotion ZHU Wenting, ZHANG Mengning, ZHAO Peiyi, WANG Ziming, SHI Yan, SUN Bingjian, CHEN Linlin, LI Honglian Chinese Journal of Biological Control    2025, 41 (2): 373-383.   DOI: 10.16409/j.cnki.2095-039x.2025.02.027 Abstract （299）      PDF（pc） （3702KB）（783）       Knowledge map    Save Fusarium crown rot (FCR), caused by Fusarium pseudograminearum, is recognized as one of the most destructive wheat diseases in China. Isolating and screening antagonistic bacteria from wheat rhizosphere soil for FCR control represents an environmentally friendly green strategy for disease management. In this study, an antagonistic bacterium, designated FCR-Y1, that efficiently inhibited F. pseudograminearum was isolated from wheat rhizosphere soil by the plate dilution method and confrontation test. The strain FCR-Y1 was identified as Bacillus based on its morphological, physiological, and biochemical characteristics. Furthermore, phylogenetic analysis based on 16S rRNA and gyrA gene sequences placed FCR-Y1 in the same clade as Bacillus subtilis, leading to its identification as B. subtilis. The strain FCR-Y1 also exhibited inhibitory effects against several other plant pathogenic fungi. Indoor pot-culture experiments revealed that FCR-Y1 achieved both preventive and therapeutic efficacy exceeding 60% against FCR, Additionally, the bacterial suspension of FCR-Y1 effectively inhibited the germination of F. pseudograminearum conidia, and the water-soluble extracts of its fermentation filtrate contained abundant antimicrobial components. These findings suggest that B. subtilis FCR-Y1, isolated from wheat rhizosphere soil, holds promising application potential in the prevention and control of FCR. Reference | Related Articles | Metrics | Comments（0） Select Control Effect of Chinese Herbal Medicine and Potassium Phosphite on Apple Leaf Diseases and Its Impact on Leaf Physiological Characteristics LIU Jianwei, WEI Jiangwen, WU Yiting, LI Jinfeng, ZHAO Zhihui, SHI Guangliang, JIANG Yanjun Chinese Journal of Biological Control    2024, 40 (6): 1366-1374.   DOI: 10.16409/j.cnki.2095-039x.2024.02.062 Abstract （299）      PDF（pc） （412KB）（986）       Knowledge map    Save Apple rust and apple scab are leaf diseases that can significantly diminish apple production. This study aimed to develop a biocontrol strategy using a combination of traditional Chinese herbal medicine and potassium phosphite to manage these diseases. The efficacy of this combined treatment was evaluated on disease control and its impact on apple leaf growth. The results showed that the integrated approach provided control efficiencies of 61.65% and 73.00% against apple rust, and 90.91% and 98.85% against apple scab at 14 and 28 days post-final application, respectively. These rates were comparable to the application of difenoconazole 10% WG, while reducing the dosage of the Chinese herbal medicine and potassium phosphite by 10% and 24.92%, respectively, demonstrated a significant synergistic effect. The combined treatment also led to an increase in leaf area, thickness, and both fresh and dry weights, as well as enhanced levels of chlorophyll and amino acids. Additionally, it significantly boosted the activities of defense enzymes, including superoxide dismutase (SOD), polyphenol oxidase (PPO), catalase (CAT), phenylalanine ammonia-lyase (PAL), and lipoxygenase (LOX), with increases ranging from 83.73% to 503.45% over the control. The combination of Chinese herbal medicine and potassium phosphite could control the occurrence of apple rust and apple scab effectively, and improved the disease resistance of apple trees, showing great potential for green control of apple leaf diseases. Reference | Related Articles | Metrics | Comments（0） Select Laboratory Pathogenicity and Control Efficiency of Metarhizium anisopliae and Lecanicillium lecanii Co-culture against Phenacoccus solani HUANG Peng, ZHANG Jie, YAO Jinai, YU Deyi, HOU Xiangyu Chinese Journal of Biological Control    2024, 40 (6): 1267-1274.   DOI: 10.16409/j.cnki.2095-039x.2022.11.013 Abstract （295）      PDF（pc） （2687KB）（522）       Knowledge map    Save To evaluate the biocontrol potential and prospect of Metarhizium anisopliae FM-03 and Lecanicillium lecanii LL-01 co-culture against the quarantine pest Phenacoccus solani, the affinity between these two strains, the sporulation and biomass of co-culture strains, and their laboratory pathogenicity and control efficiency against the mealybug were determined in this study. Results showed that these two strains grew normally and there was no obvious antagonistic effect to each other when they were co-cultured, because the affinity between these two strains was strong. The substance accumulation and insecticidal activity of co-culture strains FM-03 and LL-01 at the inoculation ratios 2:3 were optimal and significantly higher than the single culture strain. The total sporulation and biomass after ten days’ co-culture were 5.53×1010conidia/cm2and 0.72 g, respectively, and the sporulation proportions of strains FM-03 and LL-01 were 49.34% and 50.66%, respectively. The LC50 value against the mealybug at ten day infection was 2.84×104conidia/cm2. The cumulative mortality, co-infection proportion and LT50 values for a dose of 1×108 conidia/mL were 97.73%, 67.01% and 3.63 d, respectively. In addition, the laboratory contro1 efficiency and co-infection proportion of the co-culture strains also enhanced with time, reaching 86.14% and 60.17% at twelve days post treatment with 1×108 conidia/mL. In conclusion, strains FM-03 and LL-01 co-culture at the suitable inoculation proportions has significant synergistic effect on the control of P. solani, suggesting that these two strains have an excellent potential in the development of compound biocontrol agent. Reference | Related Articles | Metrics | Comments（0） Select Control Effect of Wuyiencin and Cyprodinil on Botrytis cinerea ZHANG Xiaocui, XU Chunli, ZHOU Qianying, LIAO Yutong, SHI Liming, GE Beibei Chinese Journal of Biological Control    2025, 41 (1): 156-164.   DOI: 10.16409/j.cnki.2095-039x.2025.02.004 Abstract （290）      PDF（pc） （2563KB）（558）       Knowledge map    Save Biological pesticides have been demonstrated to be both safe and highly effective. The combination of these biopesticides with chemical pesticides allow for a reduction in the application dosage of chemicals while simultaneously increasing control efficacy. In this study, the synergistic combination of wuyiencin and cyprodinil was screened out. The effects on mycelial growth and conidial germination of Botrytis cinerea under optimal combination conditions were determined. Additionally, the efficacy of this combination in controlling Botrytis cinerea on detached leaves of grape was evaluated. The results showed that the synergistic effect was the most significant when the ratio of wuyiencin to cyprodinil was 9:1, and the toxocity ratio was 1.678. The combination caused hyphae expanded irregularly and grew abnormally, significantly inhibited mycelial growth, and impeded conidial germination of Botrytis cinerea. The control efficacy on detached grape leaves and fruits was 75.19% and 66.67%, respectively, exhibiting a significant increase of 16.53%－27.06% and 19.05%－30.16% compared to the single treatment. This study provides a theoretical basis for the enhanced control efficacy of Botrytis cinerea through the combination of wuyiencin and cyprodinil. It also offers technical guidance for the environmentally friendly and efficient management strategies. Reference | Related Articles | Metrics | Comments（0） Select Impacts of Bee Pollens Consumption on Reproductive Fitness and Offspring Population Dynamics in Chrysoperla sinica Adults LI Fulin, SONG Jingrong, GUO Lian, ZHANG Min, ZHANG Pengjiu, HE Lifei, YANG Jing, GAO Yue, LIU Zhongfang Chinese Journal of Biological Control    2024, 40 (6): 1237-1242.   DOI: 10.16409/j.cnki.2095-039x.2024.11.002 Abstract （289）      PDF（pc） （427KB）（787）       Knowledge map    Save This study examined the impact of different bee pollens on reproductive fitness and offspring population dynamics of Chrysoperla sinica. Results showed that feeding on camellia bee pollen extended the pre-oviposition period of C. sinica adults to 6.00 days and significantly increased the total number of eggs laid to 228.30 per female, compared with the control group (5.00 days and 113.90 eggs). Corn bee pollen led to an average of 216.10 eggs per female, not significantly different from camellia bee pollen, but higher than the control group. However, rape bee pollen reduced female adults’ longevity to 12.20 d. All three treatments shortened the larval duration of the offspring significantly. Cocoon weight under camellia and corn bee pollen treatments was significantly higher than that of rape bee pollen treatment. In addition, camellia and corn bee pollen significantly improved the fecundity and longevity of offspring adults. Specifically, the total number of eggs laid per female increased from 116.80 in the control group to 275.70 and 253.40, respectively, while the longevity of female adults was extended from 20.45 d to 23.35 d and 25.70 d, respectively. After being treated with camellia and corn bee pollen, the net reproductive rate (R0) of the offspring population significantly increased from 29.63 in the control group to 63.42 and 61.35, respectively. Meanwhile, intrinsic rate of increase (rm) and finite rate of increase (λ) also exhibited notable enhancements, rising from 0.09 and 1.10 in the control group to 0.12, 1.12, and 0.11, 1.12, respectively. The population doubling time (Dt) was significantly shortened to 5.94 d and 6.08 d, respectively, compared with the control group (7.25 d). In conclusion, feeding C. sinica adults with corn bee pollen and camellia bee pollen can promote their fecundity and offspring population growth. These findings provide scientific basis for improving the indoor breeding efficiency of C. sinica. Reference | Related Articles | Metrics | Comments（0） Select Research Progress on Biological Control of Root Rot Disease in Root- and Rhizome-Derived Traditional Chinese Medicines XIAO Ouli, WANG Jiale, CHEN Jieyin, DAI Xiaofeng, KONG Zhiqiang Chinese Journal of Biological Control    2025, 41 (3): 511-519.   DOI: 10.16409/j.cnki.2095-039x.2025.02.037 Abstract （282）      PDF（pc） （976KB）（864）       Knowledge map    Save With the scarcity of wild medicinal plant resources and the increase in market demand, conventionally cultivated medicinal plants have become the dominant source in the market. However, due to soil degradation, imbalance in the rhizosphere microbial community, and the accumulation of allelopathic and autotoxic substances, root rot has emerged as a major disease in the cultivation of root- and rhizome-derived traditional Chinese medicines (RR-TCMs) in single, intensive, large-scale cultivation. It has a high incidence rate and is difficult to control, severely affecting the yield and quality of traditional Chinese medicines (TCMs). Currently, the application of microbial biocontrol agents in the control of diseases and pests of medicinal plants offers promising prospects for the sustainable development of TCMs. This paper presents a review of the antagonistic microorganisms and biological control mechanisms of root rot disease in RR-TCMs, aiming to provide a reference for the control of root rot. Reference | Related Articles | Metrics | Comments（0） Select Effects of High Temperature on Two Species of Ladybugs and Their Control of Myzus persicae on Pepper in Greenhouse WU Xinglong, ZHANG Jingrong, SONG Zhanfeng, LIU Yushan, YONG Yanping, LI Sihan, YAN Zhenpeng, YANG Minglu, PU Deqiang Chinese Journal of Biological Control    2025, 41 (1): 7-14.   DOI: 10.16409/j.cnki.2095-039x.2024.07.012 Abstract （273）      PDF（pc） （430KB）（505）       Knowledge map    Save Aphids are important pests of greenhouse vegetables, and chemical pesticides are widely used to control them. Release of ladybugs for the control of Myzus persicae can reduce the use of chemical pesticides, but high temperatures in the greenhouse may backfire the ladybugs performance. In this study, the survival rate and fecundity of two species ladybugs exposed to high temperature treatment were measured together with their control of M. persicae on pepper plants in greenhouses. The results showed that Coccinella septempunctata was able to reproduce normally at a variable temperature of 25℃ /37℃, but failed to reproduce at a constant temperature of 37 ℃, whereas Hippodamia variegata was able to reproduce normally at both a constant temperature of 37 ℃ and a variable temperature of 25℃ /37℃ . The occurrence of M. persicae on pepper plants increased since the beginning of June, peaked in late July, and then gradually decreased. The results of field experiments showed: eggs of C. septempunctata pretreated at variable temperature of 25/37 ℃ hatched at 46.8% in contrast to 88.3% at 25 ℃, which afforded a 88.5% control of M. persicae on pepper plants in contrast to 69.0% at 25 ℃ at 30 days post release of the eggs. Eggs of H. Variegata pretreated at variable temperature of 25℃ /37℃ and constant temperature of 37 ℃ hatched at 85.3% and 43.03%, respectively, in contrast to 79.1% at 25 ℃, which afforded 97.8% and 77.6% control of M. persicae on pepper plants, respectively, in contrast to 96.9% at 25 ℃ at 30 days post release of the eggs. This study shows that both species of ladybugs have to ability to adapt to and reproduce at high temperature of 37 ℃, and can be used in the control of M. persicae on pepper plants in greenhouses, although H. Variegata is a better choice than C. septempunctata. Reference | Related Articles | Metrics | Comments（0） Select Comparative Analysis of Microbial Pesticides Registration in China and United States LI Yonghua, WANG Xinfang, ZHANG Wei, LIU Mei, WANG Chenfang, WANG Xuncheng, YAN Jiye Chinese Journal of Biological Control    2024, 40 (6): 1386-1397.   DOI: 10.16409/j.cnki.2095-039x.2024.02.068 Abstract （266）      PDF（pc） （2685KB）（855）       Knowledge map    Save Microbial pesticides, known for their high efficiency, low risk, specificity, and environmental friendliness, have become a global strategic emerging industry. This paper compared the registered active ingredients of microbial pesticides in China and the United States, revealing that China has registered a total of 45 bacterial, fungal, and viral microbial pesticide active ingredients, accounting for 35.43% of those registered in the United States. Specifically, the number of bacterial, fungal and viral ingredients of China accounted for 30.00%, 26.67% and 100.00% of that in the United States, respectively. China's registered active ingredients involved 39 species and subspecies, representing 55.71% of those in the United States, with bacterial ingredients accounting for 59.26%, fungal ingredients for 34.38%, and viral ingredients for 109.09%. Among the species and subspecies registered only in one country, China has 19 unique entries, making up 38.00% of those in the United States, with bacteria accounting for 42.11%, fungi for 8.70%, and viruses for 112.50%. This study aims to provide important references for the development of microbial pesticides in China. Reference | Related Articles | Metrics | Comments（0） Select Effect of Photoperiod on Growth and Development and Reproductive Behaviors of Spodoptera exigua (Hübner) PAN Jilong, SUN Qingming, SU Honghua Chinese Journal of Biological Control    2024, 40 (6): 1302-1309.   DOI: 10.16409/j.cnki.2095-039x.2024.03.010 Abstract （263）      PDF（pc） （442KB）（397）       Knowledge map    Save In order to clarity the effects of photoperiod on the growth, development and reproductive behaviours of Spodoptera exigua, three different photoperiods (12L:12D, 16L:8D, 20L:4D) were set up in the study. The results showed that, at 20 h of light duration, the hatching rate of S. exigua was only 74.0%; the larval during was shortened to 11.5 d, the survival rate of larvae was reduced to 57.8%, the pupation rate was only 57.8%, the pupal weight was reduced to 0.1905 g and the adult life span was shortened to 6.3 d. Under the condition of sex ratio of 1:1, the reproductive behavioral parameters were observed continuously in seven scotophases. In the third scotophase, the length of time between the onset of courtship and the scotophase was four times longer for S. exigua in 12 h treatment (212.4 min) than in 20 h treatment (51.4 min), the number of courtships under 12L:12D, 16L:8D and 20L:4D were 47.7, 37.9 and 25.5, respectively, and all three treatments were significantly different from each other, the mating initiation time was shortened or reduced with the increase of light time. Egg production at 12 h light (855.0) was approximately 2.5 times that at 20 h light (301.6). The results of this study preliminarily revealed the influence of photoperiod on the growth, development and reproductive behavior of S. exigua, which can provide data for indoor artificial breeding or field management of S. exigua. Reference | Related Articles | Metrics | Comments（0） Select Searching and Parasitism Behavior of Trichogramma chilonis Ishii on Vacuum-Packed and Refrigerated Rice Moth Eggs MEI Wenhao, LI Huan, FAN Rui, ZHU Wenya, WANG Yi, MA Ruiyan, ZHANG Ye Chinese Journal of Biological Control    2024, 40 (6): 1422-1429.   DOI: 10.16409/j.cnki.2095-039x.2024.02.065 Abstract （262）      PDF（pc） （778KB）（352）       Knowledge map    Save The eggs of Corcyra cephalonica serve as an ideal intermediate host for Trichogramma chilonis in indoor rearing systems. However, both the storage method and duration of the eggs significantly influence the host preference and parasitism behavior of the parasitoid. This study utilized a stereomicroscope and video recording system to observe and analyze the searching and parasitism behaviors of T. chilonis on C. cephalonica eggs stored under two conditions: regular refrigeration and vacuum-packed refrigeration. The findings revealed that for storage durations of 0, 3, 6, and 9 days, T. chilonis exhibited a decline in entry frequency into the egg areas. The highest total and single parasitism rates were recorded on day 6, reaching 7.65 and 6.44 eggs, respectively. By day 25, despite the highest entry frequency, the total and single parasitism rates decreased to 4.00 and 1.08 eggs, respectively. For storage durations of 15, 20, and 25 days, vacuum-packed refrigerated eggs required longer detection and parasitism times but resulted in higher numbers of parasitized eggs. Specifically, on day 15, the detection time was 20.37 seconds, with a parasitism rate of 2.24 eggs; on day 20, parasitism time increased to 50.27 seconds, with a rate of 1.95 eggs; and by day 25, the parasitism rate declined to 1.70 eggs. While no significant differences in parasitism rates were observed between the two storage methods during the initial 0–11 parasitism events, a clear preference for vacuum-packed refrigerated eggs emerged during the 12th and 13th events. Overall, the searching efficiency of T. chilonis’s decreased with prolonged storage time, and vacuum- packed refrigeration proved to be a superior method for preserving C. cephalonica eggs at later stages. These results highlight vacuum-packed refrigeration as a viable alternative to regular refrigeration for extending the shelf life of C. cephalonica eggs in biological control programs. Reference | Related Articles | Metrics | Comments（0） Select Predation Abilities of Mite Anystis baccarum on Spider Mites Tetranychus turkestani and Tetranychus urticae LI Mengdie, TANG Yang, LI Kun, WANG Jiahua, LI Yisong Chinese Journal of Biological Control    2025, 41 (1): 15-23.   DOI: 10.16409/j.cnki.2095-039x.2024.07.001 Abstract （257）      PDF（pc） （422KB）（534）       Knowledge map    Save Tetranychus turkestani (Ugarov et Nikolski) and Tetranychus urticae (Koch) are the main pests of cotton. Anystis baccarum (Linnaeus) is an important predatory natural enemy of agricultural and forestry pests and mites. In order to clarify the predatory effect and biological control potential of A. baccarum on T. turkestani and T. urticae, the predatory selectivity and functional response of A. baccarum to each stage of the two spider mites and the interference effect of the density of A. baccarum on its predation were determined under laboratory conditions. The results showed that A. baccarum preferred the juvenile and nymph of T. turkestani and T. urticae, with selection coefficients Q of 1.87, 1.04 and 1.79, 1.12, respectively. The functional responses of A. baccarum to T. turkestani and T. urticae fitted well with Holling II type, and the predatory ability of A. baccarum to T. turkestani and T. urticae juveniles was the strongest, with a/Th of 18.751 and 25.391, respectively. The predatory ability on nymphs was the second, with a/Th of 9.182 and 10.919, respectively. The a/Th on T. urticae juveniles and nymphs were higher by 35.4% and 18.9% than those on T. turkestani, respectively. The 1/Th (43.478, 27.029) on T. urticae juveniles and nymphs were higher by 21.7% and 18.9% than those on T. turkestani (35.718, 22.727), respectively. A comprehensive comparison of the a/Th and 1/Th on the two spider mites showed that the predatory ability of A. baccarum on T. urticae was stronger than that of T. urkestani. There was strong self-interference response and competition when A. baccarum preyed on the two spider mites, and the intraspecific interference was greater when T. turkestani was the prey. In summary, A. baccarum has good predatory ability on T. turkestani and T. urticae and is a potential biological control agent worthy of propagation. Reference | Related Articles | Metrics | Comments（0） Select Identification and Biological Characteristics of Bacillus velezensis ZY1 against Bacterial Fruit Blotch YANG Yuwen, LIU Dehua, WANG Chengliang, MENG Yonghong, GUAN Wei, ZHAO Tingchang Chinese Journal of Biological Control    2024, 40 (6): 1430-1438.   DOI: 10.16409/j.cnki.2095-039x.2024.02.064 Abstract （255）      PDF（pc） （1629KB）（418）       Knowledge map    Save The strain ZY1 was isolated from pepper leaves, which has a significant antagonistic effect on bacterial fruit blotch. Based on morphological observation, Biolog analysis, and multi gene phylogenetic analysis, strain ZY1 was identified as Bacillus velezensis. To evaluate the stress resistance of strain ZY1, its growth status was studied under different pH, NaCl concentration, and temperature. The results showed that strain ZY1 growed rapidly and has strong stress resistance. It could grow normally under conditions of pH 5-9, NaCl concentration of 2%-7%, and 25 ℃-40 ℃, and the growth of the strain was significantly inhibited at 55 ℃. The antibacterial experiment results showed that strain ZY1 had strong antibacterial effects on both group I and group II strain Aacs. The enzymatic test results showed that strain ZY1 could produce metabolites such as cellulase, pectinase, protease, and amylase. At the same time, the strain also had the ability to hydrolyze phosphorus and produce ammonia. This article reported for the first time a strain of Bacillus velezensis, which had antibacterial activity against bacterial fruit blotch. This strain ZY1 provided a new microbial resource for the biological control and soil improvement. Reference | Related Articles | Metrics | Comments（0）