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Published in last 1 year |  In last 2 years |  In last 3 years |  All Please wait a minute... For Selected: Download Citations EndNote Ris BibTeX Toggle Thumbnails Select Terminologies and Definitions of Microbial Pesticides WANG Yiyan, YUAN Shankui, NONG Xiangqun, LI Mei Chinese Journal of Biological Control    2022, 38 (2): 283-288.   DOI: 10.16409/j.cnki.2095-039x.2021.07.010 Abstract （240）      PDF （330KB）（695）       Knowledge map    In this paper, the common terminologies and definitions of microbial pesticides were summarized with reference to the microbial pesticide related guidelines and standards of FAO, WHO and China and in combination with the research progress of microbial classification and identification. The paper provides a reference for scientific cognition of microbial pesticides and promotion the standardized development of the microbial pesticide industry. Reference | Related Articles | Metrics Select Occurrence of Cicadella viridis and the Biology of Its Egg Parasitoids in Xinjiang ZHAO Qian, LIN Siyu, ZHULIDEZI Aishan, ZHONG Wen, HU Hongying Chinese Journal of Biological Control    2022, 38 (1): 29-41.   DOI: 10.16409/j.cnki.2095-039x.2021.03.009 Abstract （184）      PDF （2885KB）（666）       Knowledge map    Cicadella viridis (Linnaeus, 1758) (Hemiptera:Cicadellidae) is an insect pest with wide host range and attacks various crops including maize Zea mays L.. As important biocontrol agents, parasitoids usually provide efficient natural control of insect pests. This study aimed to clarify the occurrence of C. viridis as well as the biological characteristics of its egg parasitoids in Xinjiang, which may provide basic information that can benefit the conservation of native parasitic natural enemies and the biological control of C. viridis via mass-rearing and release of parasitoids. During 2014-2015, the life history of C. viridis was investigated in Manas National Wetland Park. C. viridis had two generations annually and overwintered as eggs in this region. Two egg parasitoid species of C. viridis were identified, Paracentrobia exilimaculata Hu & Lin was a worldwide first report parasitizing C. viridis and Gonatocerus longicornis Nees was a first report in China. The natural parasitism rate by the dominant egg parasitoid G. longicornis peaked in early September (74.96%) and averaged 49.70%. The eclosion rate and the sex ratio increased and then decreased with the increase of temperature from 15℃ to 40℃. The immature stage (from egg to adult emergence) took 9 to 16 days, averaging 14. 25 days. The lifespan of adult wasps fed with 15% honey solution ranged from 6 to12 days (Female:9.38 d; Male:8.50 d), and the females lived 1-2 days longer than the males. The optimal rearing conditions for G. longicornis were 30℃, 45% RH and photoperiod 14L:10D, and these conditions favored its parasitization of C. viridis. The parasitoid has high potential for the biological control against C. viridis. Reference | Related Articles | Metrics Select The Landmark Achievements of Biological Pesticides in Comprehensive Technology Development for Reduction and Efficiency Enhancement of Chemical Fertilizers and Pesticides ZHANG Hui, XU Ning, CAO Liru, ZHANG Kai Chinese Journal of Biological Control    2022, 38 (1): 1-8.   DOI: 10.16409/j.cnki.2095-039x.2022.01.006 Abstract （181）      PDF （583KB）（514）       Knowledge map    Chemical pesticides, as a necessary input in agricultural production, have greatly promoted crop yield and ensured food security since their invention. However, the abuse of chemical pesticides has caused serious damage to ecological environment and pollution to agricultural products. Biopesticides, as a category of environment-friendly pesticides, are emerging to gradually replace the chemical pesticides in recent years. From 2016 to 2018, a total of 49 projects in 12 tasks were launched with the "Comprehensive Technology Research and Development for Reduction and Efficiency Improvement of Chemical Fertilizers and Pesticide" project, which was aimed to solve the problems of excessive application of chemical fertilizers and pesticides and backward product development. The implementation of this project greatly promoted green pesticide research and application in China. The product development, patent application, standard formulation, monograph publication, and application of biological pesticide related with the project were introduced in this paper, hoping to provide references for relevant researchers, production and business entities, administrative departments and users. Reference | Related Articles | Metrics Select Review on Registration and Management of Bio-pesticide in China ZHANG Hongjun, TAO Lingmei, LIU Xue, HUANG Xiuzhu Chinese Journal of Biological Control    2022, 38 (1): 9-17.   DOI: 10.16409/j.cnki.2095-039x.2022.01.012 Abstract （181）      PDF （417KB）（564）       Knowledge map    The bio-pesticides include biochemical pesticides, microbial pesticides, and botanical pesticides. Even though the percentage of bio-pesticides in the market is not very high, it is hopeful for the development of bio-pesticides. The bio-pesticides could help to reduce the usage of chemical pesticides, confirm the safety of agricultural products, ecological environment and pest control in the specific crops. The recent status of bio-pesticides registration management, the achievements of the bio-pesticides industry, some problems were summarized and discussed in this paper. Some helpful suggestions for the development of bio-pesticides in the future were also pointed out for the reference of the relative stakeholders for bio-pesticides. Reference | Related Articles | Metrics Select Progress and Prospect of Microbial Metabolite Pesticides Research, Development and Application in China HE Ya-Wen, LI Guangyue, TAN Hong, KANG Qianjin, GE Beibei, ZHAO Yangyang, ZHANG Kecheng, JIANG Xiliang, LIU Fengquan, LI Yaning, ZHANG Hongyan, BAI Lingquan, XIANG Wensheng, QIU Dewen, YANG Ziwen, DENG Zixin Chinese Journal of Biological Control    2022, 38 (3): 537-548.   DOI: 10.16409/j.cnki.2095-039x.2022.02.028 Abstract （160）      PDF （1923KB）（375）       Knowledge map    Microbial metabolite pesticide (MMP) contains the microbial fermentation metabolite as the main active component and is effective in the prevention of both plant disease and weed problems or in the promotion of plant growth and development. MMPs mainly include the agricultural antibiotics, plant immune elicitor and plant growth regulator, they are the most widely used biopesticides in China. MMP has both preventive and therapeutic effects on plant disease, therefore, it is one of the important areas for the future development of green pesticides. In this review we summarize the major MMPs, their characteristics, and latest research and development progress. We then discuss the problems, challenges and prospects of MMP research, development and application in China, and provide a reference for the future research and development of new MMP. Reference | Related Articles | Metrics Select Development and Application of Insect Sex Pheromone Technology in China LIU Wancai, LIU Zhendong, ZHU Xiaoming, DU Yongjun Chinese Journal of Biological Control    2022, 38 (4): 803-811.   DOI: 10.16409/j.cnki.2095-039x.2022.05.010 Abstract （159）      PDF （479KB）（317）       Knowledge map    Insect sex pheromones are trace and volatile chemicals emitted by pheromone glands during calling after sexual maturation,which attract conspecific individuals to mate and are species specific.It has been widely applied in the monitoring and control of insect pests.In China,a series of great progresses have been made in the industrialization of chemical synthesis and purification,improvement of the techniques for stabilization and controlled release of pheromone compounds,the development of various traps and smart pheromone trapping and forecasting technology,and the practical application of pheromone trapping and mating disruption for various pests in the field,which meets the needs of monitoring and green control of major pests.Here,we comprehensively introduced and summarized the principle,types and application methods of pheromone lures and traps of mass trapping and dispensers of mating disruption developed in China,and their application in the field.The relationship between calling and mating,the attractiveness and specificity of sex pheromone,and the effects of biotic and abiotic factors on pheromone trapping and mating disruption were systematically summarized.The case analysis of application in important pests of vegetables,fruit trees,cotton and other crops was presented in detail.The problems found in the research and development,extension and application of pheromone technology were discussed and analyzed,and countermeasures and suggestions for improving the work were put forward. Reference | Related Articles | Metrics Select The Development of Biological Pesticides Based on RNA Interference GAO Liwen, CHEN Shiguo, ZHANG Yu, QIANG Sheng Chinese Journal of Biological Control    2022, 38 (3): 700-715.   DOI: 10.16409/j.cnki.2095-039x.2021.11.015 Abstract （153）      PDF （510KB）（521）       Knowledge map    RNA interference, also known as post transcriptional gene silencing, is an emerging new genetic engineering technique that can effectively silence or inhibits the expression of target genes. Biopesticides based on RNA interference are considered as a revolutionary technique in the field of plant protection in the future, which will completely change the ideas and strategies of controlling agricultural diseases, insects, weeds and other pests. Here, the basic principle and development process of RNA interference were briefly reviewed. The current status of research and application of RNAi biopesticides was summarized. The opportunities and challenges to develop RNAi biopesticides as well as its development prospect were deeply analyzed. This review serves to provide some valuable references for the development of RNAi biopesticides in China. Reference | Related Articles | Metrics Select Review on the Occurrence and Management Technology of Ceracris kiangsu in China LI Hongmei, WANG Junya, ZHUO Fuyan, ZHU Jingquan, TU Xiongbing, ZHANG Guocai, BELINDA Luke Chinese Journal of Biological Control    2022, 38 (2): 531-536.   DOI: 10.16409/j.cnki.2095-039x.2021.05.001 Abstract （133）      PDF （396KB）（553）       Knowledge map    The yellow-spined bamboo locust, Ceracris kiangsu, usually damages bamboo forests historically. C. kiangsu invaded Yunnan Province of China from Laos and Vietnam in June, 2020 and has since caused serious damage to agricultural crops there. This paper reviewed the history and current situation of occurrence of C. kiangsu, and then analyzed the causes of serious occurrence and the main control measures against different development stages of C. kiangsu. In the end, the research progresses of the natural enemies and biopesticides for control of C. kiangsu were specifically summarized, with the aim to aid the management and control of C. kiangsu in agricultural areas in the future. Reference | Related Articles | Metrics Select Predation Capacity and Preferences of Orius similis Zheng for Three Aphid Species DUAN Pan, WU Daohui, HU Changxiong, HE Menglan, SHEN Zixian, CHEN Guohua, YIN Honghui, XU Tianyang, YANG Jinbo, ZHANG Xiaoming Chinese Journal of Biological Control    2022, 38 (1): 149-158.   DOI: 10.16409/j.cnki.2095-039x.2021.05.006 Abstract （128）      PDF （460KB）（532）       Knowledge map    Orius similis Zheng (Hemiptera:Anthocoridae) is an important predatory natural enemy against tiny pest insects in agro-ecosystems in south China. The predation capacity and preference of O. similis to main aphid species on winter crops in Yunnan province were determined in 5 th instar nymphs, male adults, and female adults of O. similis using different mixed aphids groups under laboratory conditions. Three main aphid species Myzus persicae (Hemiptera:Aphididae), Brevicoryne brassicae (Hemiptera:Aphididae) and Acyrthosiphon pisum (Hemiptera:Aphididae) on winter crops in Yunnan province were selected as mixed prey A (hereinafter called MA), M. persicae parasitized by Aphidius gifuensis (Hymenoptera:Aphidiidae) in three different parasitic status were selected as mixed prey B (MB), and young nymphs, old nymphs and adults of M. persicae were used as mixed prey C (MC). The O. similis predated more preys of M. persicae and B. brassicae (MA), mummified aphids and healthy aphids M. persicae (MB), and low-instar nymphs and high-instar nymphs of M. persicae (MC) than those of the other aphids in the same group, showing a positive preference ( C i>0); while showed a negative preference for A. pisum (MA), low-instar parasitic aphids M. persicae (MB) and adults M. persicae (MC) ( C i<0). The total daily predation ranged between 6.71-15.08 and ranked as MA>MC>MB. O. similis had great predation capacity to aphids and can be used to control aphids on winter crops. However, O. similis stands a high risk to predate mummified M. persicae, therefore O. similis should be released after peak formation period of mummified and eclosion of offspring A. gifuensis. Meanwhile, under the coexistence of mixed preys, the total daily predation of O. similis is affected by the complexity of aphid species and types of mixed prey. Reference | Related Articles | Metrics Select Contact Toxicity of Two Essential Oils Extracted from Ajania against Ditylenchus destructor XIE Ziyi, LU Peiyu, NING Anqi, LIU Xinying, WANG Junlong, ZHANG Ji, LIANG Junyu Chinese Journal of Biological Control    2022, 38 (3): 549-554.   DOI: 10.16409/j.cnki.2095-039x.2021.07.012 Abstract （122）      PDF （393KB）（454）       Knowledge map    The control effect of the essential oils extracted from Ajania against Ditylenchus destructor was investigated through testing the nematocidal activity of the essential oils of A. nematoloba and A. nitida and their main components against D. destructor. The two essential oils, eucalyptus and verbenenol, and their mixture were applied separately by the drug immersion method to D. destructor. Results showed that the essential oils of A. nematoloba and A. nitida had certain insecticidal activity against D. destructor, with LC 50 of 1.50 mg/mL and 1.23 mg/mL, respectively, at 24 h post treatment, which were significantly lower than that of the positive control carbofuran. At 48 h post treatment, LC 50 of the essential oils were 1.06 mg/mL and 0.70 mg/mL, respectively. At 24 h post treatment, the nematocidal activity of eucalyptus oil (LC 50=0.49 mg/mL) and verbenenol (LC 50=1.09 mg/mL) were stronger than those of the two essential oils. When eucalyptus and verbenenol were mixed at the volume ratios of 2:6, 4:4 and 3:5, the mixtures showed LC 50 values of 0.18, 0.17 and 0.20 mg/mL, respectively, indicating a significant synergistic effect. These results indicate that the essential oils and main components extracted from Ajania are of great potential for the green prevention and control of D. destructor. Reference | Related Articles | Metrics Select Repellency of Two Volatile Pyrethroids to Aedes ZHOU Qiaoling, XU Zhanyi, YAN Ru, ZHU Guonian, CHEN Mengli Chinese Journal of Biological Control    2022, 38 (1): 215-221.   DOI: 10.16409/j.cnki.2095-039x.2021.05.021 Abstract （119）      PDF （665KB）（518）       Knowledge map    Pyrethroids, with both insecticidal and repellent activities, have been widely used in mosquito coils and other mosquito-repellent products. However, the repellent mechanism of pyrethroid remains unknown. In this study, two volatile pyrethroids, dimefluthrin and meperfluthrin, induced spatial (non-contact) repellency in Aedes aegypti and Aedes albopictus in a hand-in-cage assay. Electroantennography (EAG) recordings showed that, except dimefluthrin, other pyrethroids did not elicit EAG responses from adult antennae. Furthermore, the repellency of dimefluthrin and meperfluthrin was weaker against pyrethroid-resistant population of Aedes aegypti (Puerto Rican) carrying kdr mutations on sodium channels than against the susceptible population. Our results suggest that activation of olfactory system is not responsible for pyrethroid repellency in mosquitoes, maybe other factors, such as sodium channels, contribute to pyrethroid repellency. Reference | Related Articles | Metrics Select Chinese Journal of Biological Control    2022, 38 (2): 289-291.   DOI: 10.16409/j.cnki.2095-039x.2021.07.018 Abstract （118）      PDF （278KB）（482）       Knowledge map    Reference | Related Articles | Metrics Select Preliminary Study on the List Management of Registered Varieties of Biopesticides in China YANG Jun, HOU Yanhua, LIN Ronghua, JIANG Hui, LIU Yinchi, QIU Lihong, LIU Xue Chinese Journal of Biological Control    2022, 38 (4): 812-820.   DOI: 10.16409/j.cnki.2095-039x.2022.05.011 Abstract （111）      PDF （455KB）（241）       Knowledge map    In recent years,new varieties of biopesticides have emerged thick and fast in China,the registration heat of biopesticides has continued to rise,and the registration management of it has been constantly standardized.This paper discusses the list management of registered varieties of biopesticides in China,puts forward the scope and establishment principles of the list,combs and forms the list of registered varieties of biopesticides in China.In addition,this paper puts forward suggestions on improving the list management of registered varieties of biopesticides. Reference | Related Articles | Metrics Select Effect of Cold Storage on the Biological Characteristics of Fopius vandenboschi (Fullaway) LIN Jia, Yang Deqing, HAO Xuxing, JIA Pingfan, CAI Pumo, LIU Changming, JI Qing'e Chinese Journal of Biological Control    2022, 38 (2): 292-299.   DOI: 10.16409/j.cnki.2095-039x.2021.05.016 Abstract （109）      PDF （384KB）（573）       Knowledge map    Fopius vandenboschi (Fullaway) is an important parasitic wasp of Bactrocera dorsalis and several other tephritid pests. To meet the large demand of biocontrol agents for biological control program, it is necessary to store F. vandenboschi at low temperatures. In this study, we determined the impacts of cold temperature and storage duration on the emergence parameters of F. vandenboschi pupae at different ages. Then, four optimum pupal storage protocols were developed and evaluated for their impacts on the biological characteristics of post-storage adults. Our results showed that temperature, storage duration, pupal age and their interactions all significantly affected the emergence rate of F. vandenboschi. Emergence rate was not negatively affected only when 9-day old pupae were stored at 13 ℃ for 10 d or 15 d, which were 73.10% and 68.31%, respectively. The two cold storage protocols showed no negative effects on the dry weight, flight capacity, longevity, fecundity and life table parameters of post-storage adults. This study serves as a reference for optimizing the cold storage technique of F. vandenboschi. Reference | Related Articles | Metrics Select Research Progress on Biological Ecology, Conservation and Utilization of Hippodamia variegata (Goeze) JIANG Yan, XIU Chunli, WANG Dongmei, LIU Jian, PAN Hongsheng, LIU Xiaoning Chinese Journal of Biological Control    2022, 38 (1): 50-62.   DOI: 10.16409/j.cnki.2095-039x.2021.07.020 Abstract （108）      PDF （517KB）（508）       Knowledge map    Hippodamia variegata (Goeze), one of the most important predatory natural enemies against aphids and many other pests, is characterized by early occurrence, huge population size, polyphagy and voracity. A comprehensive understanding of the biological and ecological characteristics of H. variegata is an important prerequisite for scientific utilization of its biological control potential. This paper summarizes the research progresses of H. variegata, mainly focusing on the ontogeny, bio-ecological characteristics, and predation capability, as well as conservation measures and their release application. This information may provide useful knowledge for conservation and application of H. variegata in the biological control. Reference | Related Articles | Metrics Select Influence of Plant Growth Regulators on Sporulation and Conidia Characteristics of the Entomopathogenic Fungus Beauveria bassiana CAO Weiping, ZHEN Wei, CHEN Dan, FENG Shuo, SONG Jian Chinese Journal of Biological Control    2022, 38 (3): 555-564.   DOI: 10.16409/j.cnki.2095-039x.2021.05.022 Abstract （105）      PDF （2853KB）（517）       Knowledge map    Entomopathogenic fungus Beauveria bassiana plays an important role in biological control of agricultural and forest pests. Fermentation period and sporulation quantity were regarded as the main evaluation indexes of B. bassiana fermentation. Influence of plant growth regulators on the conidial production of B. bassiana strain JG-17 was investigated. The results showed that the conidial production was significantly increased by spraying ethephon solution on the fungal colony at the vegetative growth stage. The sporulation in the fungal colony treated with 100 ng/cm 2 of ethephon on the 8 th day of cultivation was 6.28 times of that in the control. No significant differences were observed in heat resistance, UV resistance and virulence against target pests of the conidia between the ethephon treatment and the control. A reasonable application of ethephon to the culture of B. bassiana can effectively improve the sporulation efficiency of B. bassiana. The results provide a new idea to improve mass production efficiency of fungal pesticides Reference | Related Articles | Metrics Select The Relationship between Symbiotic Bacteria and Wing Dymorphism in Rhopalosiphum padi XU Chao, WANG Lisha, ZHU Xiangzhen, WANG Li, LI Dongyang, ZHANG Kaixin, JI Jichao, LUO Junyu Chinese Journal of Biological Control    2022, 38 (1): 205-214.   DOI: 10.16409/j.cnki.2095-039x.2021.05.005 Abstract （105）      PDF （2348KB）（528）       Knowledge map    The purpose of this study was to explore the relationship between symbiotic bacteria and the wing dimorphism in Rhopalosiphum padi (Linnaeus) and to enrich the understanding of the mechanism of insect phenotypic plasticity. This study used Illumina MiSeq high-throughput sequencing platform to sequence the V3-V4 regions of the bacterial 16S rDNA gene in the alate and apterous morphs of R. padi, respectively. One hundred and seventy-four operational taxonomic units (OTUs) belonging to 10 phyla, 18 classes, 42 orders, 66 families and 98 genera were identified from all samples. At the genus level, Buchnera, as the dominant bacteria, had the highest abundance (83.21%), followed by Staphylococcus (5.25%) and Arthrobacter (2.08%). The diversity of symbionts varied between the two morphs. Apterous morph had more bacterial diversity (174 OTUs belonging to 10 phyla, 18 classes, and 66 families) than alate morphs (151 OTUs belonging to 9 phyla, 14 classes, and56 families). The abundance of various OTUs were significantly different between these two morphs. Among these OTUs, only Buchnera represented a high proportion (95.57%) in alate morphs but were present in low abundance (70.84%) in apterous morphs. The survey showed that there were differences in the diversity and abundance of symbionts between winged and wingless R. padi. These results are helpful to further understand the microorganisms that may be involved in insect wing dimorphism, and contribute to control the dispersal of this pest by reinfection of symbionts or targeting symbiosis-related host genes by RNA interference in future. Reference | Related Articles | Metrics Select Species Identification and Diversity Analysis of Pathogenic Fungi Infecting Overwintering Ostrinia furnacalis in Xinjiang Oasis JIANG Xudong, DING Xinhua, WANG Xiaowu, FU Kaiyun, YUAN Zihan, TOULXUN Ahemaiti, HE Jiang, JIA Zunzun, GUO Wenchao, WANG Zhifang, YANG Xinping, DAI Jinping, XIE Yuqing, ZHOU Liuyan, FENG Lei Chinese Journal of Biological Control    2022, 38 (1): 18-28.   DOI: 10.16409/j.cnki.2095-039x.2021.09.020 Abstract （100）      PDF （510KB）（480）       Knowledge map    The diversity of pathogenic fungi resources of overwintering Ostrinia furnacalis in Xinjiang oasis was investigated from 2019 to 2020 at 185 sampling sites in 6 counties/cities from 5 prefectures in Xinjiang. A total of 136 entomopathogenic pathogens were isolated and identified from 534 collected specimens, which are from 7 orders, 7 families, 9 genus and 14 species. Among them, Aspergillus nomius (22.1%), Beauveria bassiana (19.9%), and Fusarium sp. (13.2%) were dominant species; Fusarium anthophilum (9.6%), Penicillium polonicum (2.9%), Penicillium raistrickii (1.5%), Lecanicillium attenuatum (6.6%), Fusarium equiseti (3.7%), Fusarium proliferatum (2.2%), Mortierella polycephala (5.9%), Alternaria alternata (5.2%), Acremonium strictum (2.9%) were common species; Fusarium solani (0.7%) was a rare species. The relative abundance index of the fungi was the most in District of Xinshi in Urumqi city (41.4%), followed by Changji City in Changji Prefecture (21.4%), Horgos City in Ili Prefecture (15.0%), and Shule County in Kashgar Prefecture (11.4%). The richness index ranked in order of Horgos City, Yili Prefecture (10)>Shule County, Kashgar Prefecture (6)>District of Xinshi in Urumqi City (5)>Changji City in Changji Prefecture (4). Both diversity and evenness index were high in Khorgos, whereas those of Changji City in Changji Prefecture and the District of Xinshi in Urumqi, Xinyuan County in Ili Prefecture and Changji Prefecture in Changji Prefecture, and Huoerguosi City in Yili Prefecture and Shule County in Kashgar Prefecture were similar. In the two years 2019 and 2020, the highest mortalities (28% and 14%, respectively) resulted from the fungi were recorded in Xinshi District in Urumqi. In 2019, the mortalities from dominant pathogenic fungi differed significantly among the locations; in 2020, the mortalities with Beauveria were significantly different among the locations, while those with Aspergillus and Fusarium did not differ significantly. These results are significant for developing microbial control agents with local pathogenic fungi for the sustainable control of O. furnacali in Xinjiang. Reference | Related Articles | Metrics Select Preliminary Analysis of Stability and Antimicrobial Substances in Fermentation Broth of Bacillus velezensis JK19 HUANG Wei, ZHANG Lijuan, QIN Xinzheng, WANG Ning, WANG Wei Chinese Journal of Biological Control    2022, 38 (1): 73-80.   DOI: 10.16409/j.cnki.2095-039x.2021.06.010 Abstract （99）      PDF （627KB）（420）       Knowledge map    In this study, a strain of Bacillus velezensis JK19 with independent intellectual property rights was taken as the research object, which has good antibacterial effect on tomato bacterial wilt, The antibacterial effect and stability of fermentation supernatant of strain JK19 under different temperatures, pH conditions, enzyme treatments and UV irradiation were determined by TTC double-layer plate confrontation, and the antagonistic substances were preliminarily analyzed. The results showed that the diameter of the inhibition zone in the supernatant of strain JK19 decreased significantly when the temperature exceeded 80℃. The antimicrobial substances were not resistant to strong acid and alkali, and the appropriate pH was 4.0~10.0. They were not affected by pepsin, trypsin, protease K and lipase treatment. There was no significant difference between strain JK1 and control under UV irradiation for 4h, indicating that strain JK19 had strong antibacterial stability and antibacterial activity. Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) and GC-MS/MS were used to analyze the antimicrobial substances. It was speculated that strain JK19 might inhibit the growth of bacterial wilt by producing bacitromycin D, cyclodipeptide and other antagonistic substances, which laid a theoretical foundation for the development of new biological pesticides. Reference | Related Articles | Metrics Select Biocontrol Effect of Strain CSR-2 on Maize Stalk Rot and Complete Genome Sequence Analysis ZHAO Meixi, BAI Zhongyi, ZHANG Xianghui, LIU Jinliang, PAN Hongyu, ZHANG Yanhua Chinese Journal of Biological Control    2022, 38 (1): 242-249.   DOI: 10.16409/j.cnki.2095-039x.2021.08.006 Abstract （97）      PDF （2165KB）（410）       Knowledge map    A potential biocontrol strain, CRS-2, was isolated from maize rhizosphere soil. The strain showed antagonism against Fusarium graminearum, Magnaporthe oryzae, Botrytis cinerea, Sclerotinia sclerotiorum, Cercospora zeae, Helminthosporium maydis, and Rhizoctonia solani. The strain CRS-2 could inhibit the mycelial growth, mycelial morphology and spore germination of F. graminearum. The experiment of combined CRS-2 and water and fertilizer under film drip irrigation showed that the field control efficacy of CRS-2 against maize stalk rot was 52.3%. The complete genome sequence of CRS-2 were obtained by the third-generation PacBio sequencing technology. The genome length was 3202366 bp, encoding 3007 genes with average gene length gene was 943.76 bp. A total number of 198692 high-quality long reading fragments were obtained, the circle genome map was constructed, and the annotated coding genes showed that the strain was Asaia bogorensis (GenBank accession number:CP081941). This research provides a theoretical basis for further discovering microbial resources and their metabolic gene clusters for agricultural biological control. Reference | Related Articles | Metrics