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Published in last 1 year |  In last 2 years |  In last 3 years |  All Please wait a minute... For Selected: Download Citations EndNote Ris BibTeX Toggle Thumbnails Select Effects and Colonization of Beauveria bassiana in Maize under Stress of Exserohilum turcicum Infection SUI Li, LU Yang, CHI Ruikai, ZHAO Yu, ZHANG Zhengkun, LI Qiyun Chinese Journal of Biological Control    2023, 39 (4): 804-812.   DOI: 10.16409/j.cnki.2095-039x.2022.05.008 Abstract （249）      PDF（pc） （1371KB）（618）       Knowledge map    Save This study aims to clarify the colonization ability of Beauveria bassiana in maize plants and its influence on disease resistance of host plants. B. bassiana-maize symbiosis was constructed by root drenching method and chlorophyll and nitrogen content, as well as plant height were measured to evaluate the growth promoting effect of B. bassiana colonization in maize. The resistance of B. bassiana colonization against Exserohilum turcicum infection was investigated by inoculation of the disease. The colonization and spread of B. bassiana endophytic in maize plants under E. turcicum infection stress was studied by colony counting and quantitative PCR. The results showed that the colonization of B. bassiana in maize reached 68.18%, positively promoted plant growth and significantly reduced the incidence of B. bassiana by 46.67% at 21 day after inoculation. B. bassiana could be 'recruited' from the rhizosphere soil when maize leaves were infected by E. turcicum, and B. bassiana could accumulate at leaves in turn to improve resistance against plant pathogen infection. This study has further clarified the biocontrol potential of entomogenous fungi against plant diseases as endophyte, and provides a theoretical basis for the study of colonization and induced resistance of entomogenous fungi in plant tissues. Reference | Related Articles | Metrics | Comments（0） Select Mechanism of Chaetomium globosum in Promoting Growth and Preventing Disease and Advance of Its Application and Research FENG Chaohong, LI Lijuan, ZHANG Jiaojiao, WANG Junmei, SONG Yuli, LI Haohai, XU Fei Chinese Journal of Biological Control    2023, 39 (4): 961-969.   DOI: 10.16409/j.cnki.2095-039x.2023.02.043 Abstract （201）      PDF（pc） （432KB）（661）       Knowledge map    Save Chaetomium globosum, one of common plant endophytic fungi, possesses potential biocontrol function against many plant diseases. This paper summarized the research advances of Chaetomium globosum on biocontrol effect, and its biocontrol mechanisms, which include antibiosis, growth-promoting effect, induction of plant resistance, niche and nutrient competition, and hyperparasitism. Chaetomium globosum exhibits great application potential on plant disease control and growth promotion, soil improvement, and straw degradation. Advances on the fermentation technology and genetic engineering were also illuminated. This study will provide theoretical references for the application research of Chaetomium globosum as biocontrol agent. Reference | Related Articles | Metrics | Comments（0） Select The First Report of a New Parasitic Wasp Phaedrotoma sp.2 Parasitizing Liriomyza trifolii in Hainan XING Binglin, GULINUER·Ahamaijiang, WU Shaoying, PENG Zhengqiang, YANG Lei, LÜ Baoqian Chinese Journal of Biological Control    2023, 39 (3): 740-746.   DOI: 10.16409/j.cnki.2095-039x.2022.03.029 Abstract （198）      PDF（pc） （5611KB）（410）       Knowledge map    Save Liriomyza trifoliata is one of the major invasive pests in China and is mainly distributed in tropical and subtropical areas, causing huge losses. Parasitic wasps, a large group of hymenopteran insects, play an important role in the biological control of pests. Based on this point, a general survey of wasps parasitizing L. trifoliata was carried out in Hainan Province from October to December 2021. A new species that has not been reported in China was identified by morphological identification, which belongs to the genus of Phaedrotoma (Hymenoptera: Braconidae), it was named as Phaedrotoma sp.2. Molecular identification based on the mitochondrial COI gene further confirmed the taxonomic status of the wasp, and the phylogenetic tree showed that it was clustered into the genus of Phaedrotoma. This study lays a foundation for the artificial breeding of L. trifoliata parasitoids in the future and contributes to the application of biological control of agricultural pests. Reference | Related Articles | Metrics | Comments（0） Select Research Progress on Ecological Regulation to Crop Pests using Agricultural Biodiversity YAN Xueying, WANG Hui, ZHANG Yanjun, ZHAO Jianning, YANG Dianlin, ZHANG Guilong Chinese Journal of Biological Control    2023, 39 (3): 710-717.   DOI: 10.16409/j.cnki.2095-039x.2023.05.003 Abstract （193）      PDF（pc） （393KB）（540）       Knowledge map    Save At present, in the context of protecting biodiversity, maintaining the sustainable and healthy development of ecosystems and reducing the use of fertilizers and pesticides, using agricultural biodiversity to control pests has become one of the hotspots in domestic and foreign research. Agricultural biodiversity is important for maintaining the function of agricultural ecosystem and the control of crop pests are of great significance. This paper briefly describes the concept and current situation of agricultural biodiversity, and summarizes the scientific principles or theories of pest ecological regulation of biodiversity, starting from the protection of agricultural biodiversity by non-agricultural habitats and its influencing factors on agricultural biodiversity. The methods and specific applications of ecological regulation to crop pests using agricultural biodiversity are analyzed at the three levels of farmland, farmland boundary and farmland landscape pattern, and finally put forward the development direction of ecological regulation to crop pests using agricultural biodiversity, which provides knowledge, principles, methods and technical support for effective pest ecological regulation. Reference | Related Articles | Metrics | Comments（0） Select Mrhox3 Effects on Conidiation and Tolerance in Metarhizium robertsii ZHANG Nan, WU Hao, TONG Youmin, LI Xiaojuan, HUANG Bo Chinese Journal of Biological Control    2023, 39 (3): 523-532.   DOI: 10.16409/j.cnki.2095-039x.2022.03.019 Abstract （189）      PDF（pc） （848KB）（428）       Knowledge map    Save Metarhizium robertsii, as a model entomopathogenic fungus, has been applied in biocontrol programs of insect pests. Homeobox family genes play an important role in fungal asexual reproduction, however, its functions in entomopathogenic fungi remains unclear. Quantitative real-time PCR showed that homeobox family gene Mrhox3 is highly expressed at the conidiation and conidia stages. Gene knock-out and complementation were conducted to study the function of Mrhox3, and the results indicated that deletion of Mrhox3 did not affect the mutant’s vegetative growth and pathogenicity, but significantly weakened the conidiation ability. Further studies showed that loss of Mrhox3 significantly down-regulated the expression levels of multiple conidiation -related genes, such as the centarl regulators abaA and wetA. Loss of Mrhox3 resulted in promoted conidial germination and significantly enhanced conidial tolerance to UV and heat shock stress. This study provides a foundation for further understanding the mechanism of conidiation and conidia stress resistance in Metarhizium robertsii. Reference | Related Articles | Metrics | Comments（0） Select Review and Prospects on Microbiological Pesticides Used on Grain Crops in China ZHUO Fuyan, ZHANG Hongjun, LIU Wancai, ZHANG Jie Chinese Journal of Biological Control    2023, 39 (4): 747-751.   DOI: 10.16409/j.cnki.2095-039x.2023.09.001 Abstract （184）      PDF（pc） （334KB）（335）       Knowledge map    Save Microbial pesticides are an important way to promote the green control of crop pests and diseases, and also an effective way to ensure increase production and reduction loss of grain crops. Based on the application acreage of microbial pesticides on grain crops, this paper reviewed the achievements of microbial pesticide application since 2018 in view of crops types and application regions. This paper also discussed and sorted out the existing problems with microbial pesticide application, and proposed the corresponding promotion ideas. The review serves to provide reference for the development of microbiological pesticide. Reference | Related Articles | Metrics | Comments（0） Select Parasitism Ability and Offspring Development of Sclerodermus alternatusi Yang (Hymenoptera: Bethylidae) in Different Female Oviposition Times TANG Yanlong, WANG Lina, CHEN Yan, KANG Kui, ZENG Boping, YANG Zhongqi, WEI Ke Chinese Journal of Biological Control    2023, 39 (3): 499-506.   DOI: 10.16409/j.cnki.2095-039x.2022.11.004 Abstract （178）      PDF（pc） （484KB）（376）       Knowledge map    Save Sclerodermus alternatusi is a synovigenic parasitoid in which oogenesis takes place after the female is stimulated by feeding on a host, and this is a successive process. Generally, one female parasitoid can complete several times of parasitism and thus parasitize more than one hosts in its life span. In order to compare the differences of adult parasitism ability and offspring development of S. alternatusi in different adult oviposition times, the female parasitism rate, successful parasitism rate, progeny developmental duration, brood size, and male proportion were investigated in the laboratory conditions. The results showed that S. alternatusi female could complete four times of parasitization to the host, Thyestilla gebleri larvae. There were 85% of female parasitoids could complete two times of parasitization, but only 31% and 4% of females could complete three or four times of parasitization. The parasitic ability of the female parasitoid gradually decreases with the increase of the oviposition times. The durations of egg stage showed no significant difference in different oviposition times. However, the larval stage and pupal stage of the offspring were significantly different in different oviposition times, which displaying the longest in first oviposition time and the shortest in fourth oviposition time. The numbers of female and male offspring were significantly different among different oviposition times. The female offspring numbers were 40.2 and 35.1 in the first and second oviposition time, respectively. The numbers of male offspring were also less than 10 in these two oviposition times. The numbers of female offspring were sharply declined in the third oviposition time, over 60% of offsprings were males. Moreover, all of the offspring were males in the fourth oviposition time. The mean number of female offspring of a single S. alternatusi adult was approximately 80. This study provides new references for rearing and using the S. alternatusi to control the longhorn beetles. Reference | Related Articles | Metrics | Comments（0） Select Trapping Synergism of E8, E10-12:OH with Sex Pheromone of Grapholita molesta (Busck) (Lepidoptera: Tortricidae) ZHU Mengya, XIANG Huiming, LI Xianwei, WU Dongsheng, WU Hongjian, MA Ruiyan Chinese Journal of Biological Control    2023, 39 (3): 560-566.   DOI: 10.16409/j.cnki.2095-039x.2022.03.032 Abstract （175）      PDF（pc） （412KB）（444）       Knowledge map    Save The aim of this paper was to clarify the synergistic effect of different doses of E8, E10-12:OH that is the main component of sex pheromone of Cydia pomonella on the sex pheromone of Grapholita molesta, and to provide a reference for biological control and monitoring of G. molesta. Field trapping experiments and electroantennogram (EAG) were used to explore the effect of lures, which contained different dosages of E8, E10-12:OH mixing with the sex pheromone of G. molesta, on trapping efficiency of G. molesta in the field and on the EAG responses of male G. molesta. Field experiment showed that all five doses of E8, E10-12:OH used alone had a capability to trap male G. molesta and had no significant difference in trapping number of male G. molesta. Trapping efficiency of five doses of E8, E10-12:OH mixing with G. molesta sex pheromone were higher than it. The number of G. molesta captured by adding 20 μg of E8, E10-12:OH to the sex pheromone of G. molesta was 2.86 times higher than it. Both E8, E10-12:OH and G. molesta sex pheromone could elicit the EAG responses of male G. molesta, of which the intensity was positively related with the concentration of tested compounds. E8, E10-12:OH could be used as attractant or synergist of the sex pheromone of G. molesta to improve its trapping efficiency and enhance the accuracy of monitoring the population dynamics of G. molesta. Reference | Related Articles | Metrics | Comments（0） Select Visual System and Opsin in Insect TANG Yanhong, BI Siyan, WANG Xiaodi, MA Dongfang, YANG Nianwan, GUO Jianyang, LÜ Zhichuang, LIU Wanxue Chinese Journal of Biological Control    2023, 39 (3): 718-730.   DOI: 10.16409/j.cnki.2095-039x.2023.05.004 Abstract （160）      PDF（pc） （1851KB）（992）       Knowledge map    Save Insects have a well-developed and sensitive visual system, and opsin is a basic component of animal vision, and its structure largely determines the sensitivity of visual pigments to light of different wavelengths. The duplication and differentiation of opsin genes are the main mechanisms for the production of new light pigment sensitivity. Throughout the evolution of animals, many instances of opsin gene duplication and loss have occurred, forming the sensitivity of the various animal visual systems we observe today. In recent years, extensive and in-depth research has been carried out on visual proteins. This article describes the types of photoreceptors, the color vision system of insects, the classification of visual proteins, the phenomenon of co-expression of visual proteins, the duplication and deletion of visual protein genes, and the mechanism and research status of the physiological functions of visual proteins. Reference | Related Articles | Metrics | Comments（0） Select Molecular Mechanism of Azadirachtin Inhibiting Trehalase Activity Leading to Abnormal Molting and Metamorphosis of Helicoverpa armigera Larvae CHANG Yanpeng, ZHANG Bo, GENG Zichen, WEI Jizhen, AN Shiheng, ZHAO Wenli Chinese Journal of Biological Control    2023, 39 (3): 550-559.   DOI: 10.16409/j.cnki.2095-039x.2022.03.015 Abstract （158）      PDF（pc） （1462KB）（661）       Knowledge map    Save Azadirachtin is a botanical pesticide with good control effect on Lepidoptera, such as Helicoverpa armigera. In this study, the molecular mechanism of the death of H. armigera larvae after feeding with Azadirachtin was analyzed by the laboratory virulence assay, real-time quantitative PCR (qRT-PCR), and metabolite measurement. Firstly, the phenotype, body weight, mortality and pupation rate of the 5th and 6th instar larvae which were fed with different concentrations of Azadirachtin were analyzed. The results showed that Azadirachtin resulted in decreased body weight, increased mortality and decreased pupation rate of H. armigera larvae. Furthermore, we proved that Azadirachtin inhibiting the biosynthesis and hydrolysis of trehalose, the transcripts of 20E downstream genes, and reducing the ATP content through by qRT-PCR, enzyme activity and endogenous substance contents measurement. The results of this study indicate that Azadirachtin inhibited the biosynthesis and metabolism of trehalose, decreased the transcripts of 20E pathway genes, and reduced ATP production in H. armigera, so that causing molting and metamorphosis failure, and eventually led to the death of the larvae. This study is of great significance for further exploring the mechanism of Azadirachtin-induced death in H.armigera and control of pests by Azadirachtin. Reference | Related Articles | Metrics | Comments（0） Select Comparison of Anesthesia Methods in RNAi Microinjection of Brown Planthopper ZHANG Pengwen, DUAN Zhirou, LIU Changquan, PENG Yinchuan, ZHANG Wanna, XIAO Haijun, ZHANG Jing Chinese Journal of Biological Control    2023, 39 (5): 1148-1155.   DOI: 10.16409/j.cnki.2095-039x.2022.05.009 Abstract （150）      PDF（pc） （630KB）（409）       Knowledge map    Save Microinjection is an effective and accurate quantitative method to inhibit gene expression, which has a wide range of applications in insect gene function research and pest control. Usually, the tested insects are small and vigorous, and they need to be anesthetized before injection for immobilization. After the anesthesia effect is relieved, further phenotypic determination experiments can be performed. Different anesthesia methods have different effects on the anesthesia effect, physiology, and behavior of insects. Therefore, the determination of insect anesthesia methods is of great significance to reduce errors in microinjection dsRNA interference experiments. In this study, the brown planthopper was used as the experimental subject, and the anesthetic effects of different proportions of ether and ethyl acetate mixed liquid anesthetics and low temperature anesthesia on the brown planthopper were compared, and the effects on the survival rate of brown planthopper were further determined. The results showed that the recovery time of brown planthopper was prolonged with the increase of the concentration of ethyl acetate in anesthetic treatment. The effects of different anesthesia methods on the survival rate of brown planthopper in the RNAi microinjection experiment ranked from high to low were ether:ethyl acetate (1:2), ether:ethyl acetate (1:1), ether, ether:ethyl acetate (2:1), ether:ethyl acetate (3:1), 20 min on ice, and 40 min on ice. The comprehensive evaluation showed that the mixture of ether:ethyl acetate (1:2) could be used as the optimal anesthesia treatment for the RNAi microinjection experiment of brown planthopper. Reference | Related Articles | Metrics | Comments（0） Select Infecting and Toxic Effect of Steinernema carpocapsa N-Yz1 to Fall Armyworm, Spodoptera frugiperda LIU Qin, HUANG Lixin, LU Yurong, HAN Guangjie, LI Chuanming, XIA Yang, QI Jianhang, XU Jian Chinese Journal of Biological Control    2023, 39 (3): 542-549.   DOI: 10.16409/j.cnki.2095-039x.2022.03.027 Abstract （149）      PDF（pc） （1047KB）（423）       Knowledge map    Save Spodoptera frugiperda is an important invasive pest which causing serious damage to various crops. Following the invasion and expansion, the pest makes great threaten to the crops in China. Entomopathogenic nematodes, the natural enemies specialized to the pest, are considered a potential biological control agent for fall armyworm. In the present study, the symbiotic bacterium was isolated from the Steinernema carpocapsae strain N-Yz1, and identified to the genera of Xenorhabdus nematophila. The bacteria were the main toxic factor to the larvae of S. frugiperda, led to rapid larval death by injected 100 to 200 bacterial counts per larva. The infection of nematode N-Yz1 caused rapid lethality and high mortality to juvenile larvae (the second and third instar) of fall armyworm, with infected lethal individuals appearing within 24 h of inoculation and mortalities of 97.0% and 81.94% at the dosage of 30 IJs per larva. For the elder larvae (the fourth to fifth instar), the killing speed was relative slow, with initial lethal individuals appearing at the time of 48 h post inoculation and mortalities of 81.94% and 76.39% at the time of 72 h after nematode inoculation. It was positive correlation between inoculum dosage of nematode N-Yz1 and mortality ration of larvae. All the larvae of 4th instar S. frugiperda were killed when the inoculation dose exceeds 60 IJs per larva 48 h post inoculation. N-Yz1 also had the ability to infecting pupae of S. frugiperda, at the dose of 50 and 100 IJs per pupa, the mortalities of 1st day pupae were 53.57% and 55.38%. The activity of N-Yz1 infection was affect by temperature, with disability of infection at 35 ℃and peak mortality delayed to 120 h at 15 ℃. It was indicated that N-Yz1, a strain of Steinernema, is a potential agent for the controlling to the larvae and pupae of S. frugiperda.. Reference | Related Articles | Metrics | Comments（0） Select Predatory Responses of Cheilomenes sexmaculata to Diaphorina citri HE Wancai, YE Qingqing, WANG Feifeng, HU Yuwei, LIU Jinhua, MAO Xiongxing, ZHANG Ruifeng, WANG Xingmin, QIU Baoli, LIU Yutao, SANG Wen Chinese Journal of Biological Control    2023, 39 (3): 514-522.   DOI: 10.16409/j.cnki.2095-039x.2023.05.001 Abstract （140）      PDF（pc） （449KB）（364）       Knowledge map    Save Diaphorina citri Kuwayama is an important pest of citrus, navel oranges, and other members of Rutaceae family. Cheilomenes sexmaculata (Fabricius) is a common insect natural enemy in southern China. To assess the potential of C. sexmaculata to manage D. citri, the predatory functional response, search efficacy, and self-density interference response of the larvae (1st and 4th instar) and adults against various life stages of D. citri (early, late instar nymphs, and adults) were examined. Our findings demonstrated that the predatory functional response of Holling II is present in C. sexmaculata larvae and adults. The maximum daily predation amounts of the 1st instar larvae of C. sexmaculata against the early and late instar nymphs of D. citri was 55.55 and 66.66 individuals, respectively. The maximum daily predation amounts of the 4thinstar larvae of C. sexmaculata against the early, late instar nymphs and adults of D. citri was 500, 200 and 200 individuals, respectively. The maximum daily predation amount of the C. sexmaculata adults against early, late instar nymphs and adults of D. citri was 333.33, 250 and 125 individuals, respectively. With an increase in prey density, C. sexmaculata's ability to find D. citri reduced. With a rise in interference from the predator’s density at a constant D. citri density, C. sexmaculata’s predation effectiveness fell while its sharing competition intensity increased. This study offers a theoretical foundation for employing C. sexmaculata to control D. citri and Huanglongbing in the field. Reference | Related Articles | Metrics | Comments（0） Select Research Progress on Biology, Ecology and Biological Control of Cheilomenes sexmaculata (Coleoptera: Coccinellidae) TANG Liangde, ZANG Liansheng Chinese Journal of Biological Control    2023, 39 (3): 697-709.   DOI: 10.16409/j.cnki.2095-039x.2023.05.002 Abstract （139）      PDF（pc） （467KB）（796）       Knowledge map    Save The ladybeetle Cheilomenes sexmaculata (Fabricius) (Coleoptea: Coccinellidae) is one of the dominant natural enemies of various insect pests in agroforestry ecosystem due to wide distribution, common occurrence in field, high reproductive rate, wide feeding range, excellent predation ability and strong adaptability, thus there is a bright development prospect to develop this predator. In this article we reviewed the research progress on C. sexmaculata in recent 40 years, mainly focusing on the taxonomy, biological characteristics, biological control, artificial rearing, and insecticide impacts. In addition, the prospects for the future studies and application of C. sexmaculata were also discussed. Reference | Related Articles | Metrics | Comments（0） Select Screening and Identification of Broad-Spectrum Antagonistic Trichoderma against Pathogenic Fungi for Apple and Its Field Control Effect in Orchard ZHAO Yunfu, QIAO Shuqin, WANG Haiyan, XU Haijun, ZHAO Lihua, WU Haibin, SUN Zhiqiang, WANG Jianing, LIANG Chen, b Chinese Journal of Biological Control    2023, 39 (3): 567-574.   DOI: 10.16409/j.cnki.2095-039x.2023.02.019 Abstract （135）      PDF（pc） （1589KB）（494）       Knowledge map    Save In order to screen broad-spectrum fungal antagonist against apple pathogenic fungi for apple, two Trichoderma strains YTLY-13 and YTLY-36, which had obvious antagonistic effects against four common pathogenic fungi for apple, were isolated from orchards in a variety of places in Shandong Province via plate confrontation method and growth rate method. The results indicated that YTLY-13 and YTLY-36 were Trichoderma virens and Trichoderma harzianum respectively, based on their morphological characteristics and sequence homology of translation elongation factor 1 (TEF1)，which had obvious inhibitory effect on the growth of pathogenic fungi, with the inhibition rate of 68.45%, among which the inhibition rate against Cytospora mandshurica was relatively high, reaching 73.92%. The results of field efficacy showed that the control effect of strain YTLY-36 against four fungal diseases of apple was significantly higher than that of strain YTLY-13, which was equivalent to the registered agent metiram and Bacillus subtilis, and had fast growth rate and simple fermentation, and YTLY-36 exhibited the good potential to be developed as a special broad-spectrum microbial fungicide for apple trees. Reference | Related Articles | Metrics | Comments（0） Select Screening and Biocontrol Characteristics of Endophytic Strains against Ginger Bacterial Wilt DANG Keke, PENG Junwei, SUN Yang, XIE Ziyu, DONG Yuanhua, LI Jiangang Chinese Journal of Biological Control    2023, 39 (3): 585-593.   DOI: 10.16409/j.cnki.2095-039x.2023.02.030 Abstract （134）      PDF（pc） （616KB）（531）       Knowledge map    Save Ginger bacterial wilt is a soil borne bacterial disease caused by Ralstonia solanacearum, which seriously restricts the development of ginger industry. In this study, endobacteria from ginger root were isolated, and 7 antagonistic bacteria were screened by co-culture with R. solanacearum. The antagonistic endobacteria were identified as Enterobacter arburiae and Pseudomonas indica, respectively. Antagonistic endobacteria exhibit a strong antagonistic activity against R. solanacearum, with their metabolites have an inhibitory rate of over 90% against R. solanacearum. In addition, antagonistic endobacteria also exhibit a strong iron competitive advantage, with the ferritin content in their metabolites significantly higher than that in R. solanacearum metabolites. Experiment showed that the antagonistic endobacteria could successfully colonize in ginger root, significantly reduced the density of R. solanacearum in ginger rhizosphere, and control the incidence of bacterial wilt from 90.10% to 21.43%, showing a good biological control effect. This study provided valuable microbial resources for biological control of ginger blast in southern red soil area in China. Reference | Related Articles | Metrics | Comments（0） Select Screening, Identification, Biological Characteristics of Yeast Strains and Their Antifungal Activities against Botrytis cinerea LAI Chengchun, LAI Gongti, CHEN Bingxing, Pan Ruo, Que Qiuxia, CHE Jianmei Chinese Journal of Biological Control    2023, 39 (3): 594-606.   DOI: 10.16409/j.cnki.2095-039x.2023.02.025 Abstract （134）      PDF（pc） （4325KB）（379）       Knowledge map    Save Two antagonistic strains G17515 and G31887 were screened out, which showed good biocontrol activities against Botrytis cinerea. The two yeast strains were identified as Starmerella bacillaris and Meyerozyma caribbica by morphological observation and phylogenetic tree analysis. The cultural conditions of G17515 and G31887 were as follows: 20 ℃—30 ℃ (optimum 30 ℃), pH 5—7 (optimum pH 6.0) and 0.1%—5% NaCl (optimum 0.1%). The results of in vitro culture showed that two antagonistic strains G17515 and G31887 could significantly inhibit the mycelial growth of B. cinerea, which inhibit rates were 80.86% and 69.05%, respectively. And the two strains also caused abnormalities in mycelium and spore morphology of B. cinerea. Furthermore, G17515 and G31887 could inhibit the expansion of B. cinerea on grape leaves and fruits. Their inhibitory efficiency on grape leaves reached 64.21% and 57.22%, respectively. And their biocontrol efficiency on grape fruits were 48.56% and 77.13%, respectively. In conclusion, S. bacillaris G17515 and M. caribbica G31887 showed inhibitory effects on B. cinerea, which is expected to be a strain resource for controlling gray mold disease on grapes. Reference | Related Articles | Metrics | Comments（0） Select The Effect of Different Ecological Factors on Trichogramma pintoi (Voegelé) Parasitizing Eggs of Tomato Leafminer, Phthorimaea absoluta (Meyrick) FU Kaiyun, LI Aimei, DING Xinhua, JIA Zunzun, AHMAT·Tuerxun, ROUZI·Arzigul, FENG Hongzu, LI Xiaowei, GUO Wenchao Chinese Journal of Biological Control    2023, 39 (3): 507-513.   DOI: 10.16409/j.cnki.2095-039x.2023.01.011 Abstract （132）      PDF（pc） （366KB）（465）       Knowledge map    Save Trichogramma pintoi is an egg-parasitic natural enemy of Lepidopteran pests. Clearify its ability to parastic eggs of Phthorimaea absoluta provides a sight for controling P. absoluta by T. pintoi in field. In this paper, by setting different levels of egg density of T. absoluta, temperature, humidity, presence or absence of light and the days after egg laid, the main factors that affect T. pintoi parasitizing P. absoluta eggs are evaluated. At the parasitic egg density of 30 eggs/tube, 26 ℃, 75% relative humidity, and darkness environment, the parasitic rate is the highest and reach to 93.33%. The longer the duration of egg laid by P. absoluta is, the weaker the ability of T. pintoi to parasitize it. According to the model fitting test, the parasitism of T. pintoi on P. absoluta eggs is consistent with the disc equation of Holling-Ⅱ functional response, indicating that T. pintoi is one of the potential parasitoids to control P. absoluta. Reference | Related Articles | Metrics | Comments（0） Select Intraspecific Host Discrimination and Superparasitism in Telenomus remus (Nixon), an Egg Parasitoid of Spodoptera frugiperda (J. E. Smith) XUE Taoran, PAN Jing, LIU Lihong, XU Teng, HUANG Jian, HE Yuxian, WANG Zhuhong Chinese Journal of Biological Control    2024, 40 (1): 1-.   DOI: 10.16409/j.cnki.2095-039x.2023.01.026 Abstract （128）      PDF（pc） （471KB）（236）       Knowledge map    Save Telenomus remus (Nixon) is an important egg parasitoid of Spodoptera frugiperda (J. E. Smith), a major invasive pest. In order to clarify the intraspecific host discrimination and the superparasitism of T. remus, the self-recognition (same individual) and conspecific-recognition (different individual) ability to recognize the hosts eggs parasitized at different time intervals were determined, and the superparasitism rate of single and multi-female T. remus under different host density were also investigated. The results showed no superparasitism in T. remus , either self-recognition or conspecific-recognition. After a single female T. remus parasitizing for 24 h at different parasitoid-egg ratios, the percentage of 1st instar larvae of T. remus in S. frugiperda eggs was less than 100%, and only one was found in each host egg. With multi-female T. remus and sufficient hosts, and when parasitoid-egg ratios were 1:100, 2:200, 4:400, 6:600 and 8:800, the percentage of unparasitized host eggs were 52.8%, 67.2%, 63.2%, 53.6% and 51.2%, respectively, and the percentage of host eggs with one 1st instar larva were 47.2%, 32.8%, 36.8%,46.4% and 48.8%, respectively. With multi-female T. remus and insufficient hosts, and when parasitoid-egg ratios were 2:20, 3:21, 4:20, 7:21 and 20:20, the parasitism rates of T. remus were 60.0%, 83.0%, 95.0%, 94.0% and 96.0%, respectively, and the superparasitism rates were 11.0%, 38.0%, 64.0%, 73.0% and 92.0%, respectively, the average number of 1st instar larva of T. remus in a single host egg were 0.4, 0.6, 1.1, 1.3 and 2.1, respectively, and the maximum number were 3.0, 4.0, 5.0, 6.0 and 12.0, respectively. The results show that T. remus has the ability of self and conspecific-recognition, the superparasitism of a single female T. remus does not depend on host density. When the host is sufficient, the superparasitism of multi-female T. remus does not occur, but when the host is insufficient, the parasitism and superparasitism rate, the average and maximum number of 1st instar larvae in a single host egg increase with the increase of parasitoid-egg ratios. The parasitoid-egg ratio of 4:20 is suitable for propagation of the wasp because it produces high parasitism rate, low superparasitism rate, and low internal competition. Related Articles | Metrics | Comments（0） Select A New Parasitic Wasp Closterocerus chamaeleon Founding in China Parasitizing Eucalyptus Leaf Gall Maker Ophelimus maskelli (Hymenoptera:Eulophidae) LIU Huihui, CAO Liangming, WANG Xiaoyi, LI Enjie, WU Yaojun, JIANG Xuejian, LUO Ji, YANG Zhongqi Chinese Journal of Biological Control    2023, 39 (4): 995-1000.   DOI: 10.16409/j.cnki.2095-039x.2023.01.020 Abstract （127）      PDF（pc） （1136KB）（376）       Knowledge map    Save A serious invasive pest, Ophelimus maskelli (Ashmead) (Hymenoptera:Eulophidae), occurred in China in recent years. The pest forms many small galls on the leaves of Eucalyptus trees and caused defoliation. For biological control of the pest, investigation of its natural enemy was carried out. An effective parasitoid species, Closterocerus chamaeleon (Girault) (Hymenoptera:Eulophidae), was discovered by rearing the Eucalyptus leaves with galls. It is the first record of the parasitoid in China and thus was morphologically described in detail in the present paper with color photos provided. Meanwhile, the diagnostic characters that distinguish the new parasitoid from other close species in the genus were provided. The parasitoid species originally distributes in Australia and now has invaded into many countries in the world where the trees are cultivated, where the parasitoid is a superior natural enemy for the gall maker pest. Therefore, the parasitoid species could play an important role in managing the pest in China. Reference | Related Articles | Metrics | Comments（0）