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Published in last 1 year |  In last 2 years |  In last 3 years |  All Please wait a minute... For Selected: Download Citations EndNote Ris BibTeX Toggle Thumbnails Select Comparison of Anesthesia Methods in RNAi Microinjection of Brown Planthopper ZHANG Pengwen, DUAN Zhirou, LIU Changquan, PENG Yinchuan, ZHANG Wanna, XIAO Haijun, ZHANG Jing Chinese Journal of Biological Control    2023, 39 (5): 1148-1155.   DOI: 10.16409/j.cnki.2095-039x.2022.05.009 Abstract （171）      PDF（pc） （630KB）（497）       Knowledge map    Save Microinjection is an effective and accurate quantitative method to inhibit gene expression, which has a wide range of applications in insect gene function research and pest control. Usually, the tested insects are small and vigorous, and they need to be anesthetized before injection for immobilization. After the anesthesia effect is relieved, further phenotypic determination experiments can be performed. Different anesthesia methods have different effects on the anesthesia effect, physiology, and behavior of insects. Therefore, the determination of insect anesthesia methods is of great significance to reduce errors in microinjection dsRNA interference experiments. In this study, the brown planthopper was used as the experimental subject, and the anesthetic effects of different proportions of ether and ethyl acetate mixed liquid anesthetics and low temperature anesthesia on the brown planthopper were compared, and the effects on the survival rate of brown planthopper were further determined. The results showed that the recovery time of brown planthopper was prolonged with the increase of the concentration of ethyl acetate in anesthetic treatment. The effects of different anesthesia methods on the survival rate of brown planthopper in the RNAi microinjection experiment ranked from high to low were ether:ethyl acetate (1:2), ether:ethyl acetate (1:1), ether, ether:ethyl acetate (2:1), ether:ethyl acetate (3:1), 20 min on ice, and 40 min on ice. The comprehensive evaluation showed that the mixture of ether:ethyl acetate (1:2) could be used as the optimal anesthesia treatment for the RNAi microinjection experiment of brown planthopper. Reference | Related Articles | Metrics | Comments（0） Select Intraspecific Host Discrimination and Superparasitism in Telenomus remus (Nixon), an Egg Parasitoid of Spodoptera frugiperda (J. E. Smith) XUE Taoran, PAN Jing, LIU Lihong, XU Teng, HUANG Jian, HE Yuxian, WANG Zhuhong Chinese Journal of Biological Control    2024, 40 (1): 1-.   DOI: 10.16409/j.cnki.2095-039x.2023.01.026 Abstract （155）      PDF（pc） （471KB）（565）       Knowledge map    Save Telenomus remus (Nixon) is an important egg parasitoid of Spodoptera frugiperda (J. E. Smith), a major invasive pest. In order to clarify the intraspecific host discrimination and the superparasitism of T. remus, the self-recognition (same individual) and conspecific-recognition (different individual) ability to recognize the hosts eggs parasitized at different time intervals were determined, and the superparasitism rate of single and multi-female T. remus under different host density were also investigated. The results showed no superparasitism in T. remus , either self-recognition or conspecific-recognition. After a single female T. remus parasitizing for 24 h at different parasitoid-egg ratios, the percentage of 1st instar larvae of T. remus in S. frugiperda eggs was less than 100%, and only one was found in each host egg. With multi-female T. remus and sufficient hosts, and when parasitoid-egg ratios were 1:100, 2:200, 4:400, 6:600 and 8:800, the percentage of unparasitized host eggs were 52.8%, 67.2%, 63.2%, 53.6% and 51.2%, respectively, and the percentage of host eggs with one 1st instar larva were 47.2%, 32.8%, 36.8%,46.4% and 48.8%, respectively. With multi-female T. remus and insufficient hosts, and when parasitoid-egg ratios were 2:20, 3:21, 4:20, 7:21 and 20:20, the parasitism rates of T. remus were 60.0%, 83.0%, 95.0%, 94.0% and 96.0%, respectively, and the superparasitism rates were 11.0%, 38.0%, 64.0%, 73.0% and 92.0%, respectively, the average number of 1st instar larva of T. remus in a single host egg were 0.4, 0.6, 1.1, 1.3 and 2.1, respectively, and the maximum number were 3.0, 4.0, 5.0, 6.0 and 12.0, respectively. The results show that T. remus has the ability of self and conspecific-recognition, the superparasitism of a single female T. remus does not depend on host density. When the host is sufficient, the superparasitism of multi-female T. remus does not occur, but when the host is insufficient, the parasitism and superparasitism rate, the average and maximum number of 1st instar larvae in a single host egg increase with the increase of parasitoid-egg ratios. The parasitoid-egg ratio of 4:20 is suitable for propagation of the wasp because it produces high parasitism rate, low superparasitism rate, and low internal competition. Related Articles | Metrics | Comments（0） Select Control of Ostrinia furnacalis Differed between Field Release Methods of Trichogramma dendrolimi DU Wenmei, PANG Jiayao, WANG Lin, ZHANG Junjie, RUAN Changchun Chinese Journal of Biological Control    2023, 39 (6): 1275-1281.   DOI: 10.16409/j.cnki.2095-039x.2023.01.044 Abstract （135）      PDF（pc） （1101KB）（260）       Knowledge map    Save To improve the control of Ostrinia furnacalis showing new situation of occurrence and damage in Jilin Province, we conducted tests for optimal field release methods for Trichogramma dendrolimi from 2018 to 2020. The field tests showed significant differences in the control afforded by the Trichogramma released by different methods. Either increasing the number or the amount of Trichogramma release improved the control efficiency. When T. dendrolimi was used continuously to control the first and second generations of O. furnacalis, the control efficiency reached the highest of 79.32%. It is recommended that for control of the first generation of O. furnacalis, 15000 T. dendrolimi wasps be released in three times at quota of 3000, 7000, and 5000 wasps, respectively, at 2 release points each time per 667 m2. For the second generation of O. furnacalis, 10000 T. dendrolimi wasps are released once at 2 release points per 667 m2. In our practice, T. dendrolimi was released by plant protection UAV in an area of 1334 hm2 for control of O. furnacalis, the control efficiency reached 76.36%―81.23%. Reference | Related Articles | Metrics | Comments（0） Select Effect of the Global Regulator MtrAsbh on the Biosynthesis of Milbemycins ZHU Yajie, WANG Jiabin, LI Shanshan, XIANG Wensheng, ZHANG Yanyan Chinese Journal of Biological Control    2023, 39 (5): 1083-1093.   DOI: 10.16409/j.cnki.2095-039x.2022.03.030 Abstract （130）      PDF（pc） （694KB）（439）       Knowledge map    Save MtrA is a global response regulatory protein that is highly conserved in Streptomyces. The function of MtrA from Streptomyces bingchenggensis (MtrAsbh) in the biosynthesis of biopesticide milbemycins was studied by by gene deletion, complementation, overexpression, transcriptional analysis and gel retardation assay. Results showed that the mtrAsbh deletion mutant displayed a complete loss of milbemycin production, while overexpression of mtrAsbh significantly promoted the production of milbemycin, indicating that MtrAsbh is a key activator of milbemycin biosynthesis. Our results further show that MtrAsbh regulates milbemycin production by affecting the expression of milbemycin biosynthetic gene clusters and genes related to precursor supply. In addition, MtrAsbhcan act as an activator or a repressor to differentially control the expression of many other natural product biosynthesis genes presented in the genome of Streptomyces bingchenggensis. Reference | Related Articles | Metrics | Comments（0） Select Identification of Parasitic Wasp Species of Nipaecoccus vastator (Maskell) DONG Zhigang, HE Yanhuai, LUO Ye, HUANG Jian, WANG Zhuhong Chinese Journal of Biological Control    2023, 39 (5): 1001-1014.   DOI: 10.16409/j.cnki.2095-039x.2023.01.024 Abstract （125）      PDF（pc） （3486KB）（226）       Knowledge map    Save Nipaecoccus vastator (Maskell) [=Nipaecoccus viridis (Newstead)] is an important pest infesting citrus and other fruit trees and ornamental garden plants. This study investigated the parasitic wasp resources of N. vastator in Fujian, China. The parasitic wasp species of the mealybug in the world were reorganized to include the primary parasitoids with 8 genera and 25 species of Encyrtidae, the first hyperparasitoids with 4 genera and 13 species of Encyrtidae and 1 genus and 1 species of Aphelinidae, and the second hyperparasitoids with 1 genus and 1 species of both Eulophidae and Signiphoridae. Moreover, the parasitic wasp population and the food chain relationship between the parasitoids and the mealybug were analyzed, and the first and the second hyperparasitoids were identified for the first time. At the same time, the known 8 species of parasitoids associated with N. vastator from China were clarified, a key to these parasitoid species was provided, and the morphological characteristics of the main parasitoid species were described with photos, including 1 newly recorded species for China, Anagyrus mirzai Agarwal et Alam. Reference | Related Articles | Metrics | Comments（0） Select Isolation, Identification, and Pathogenicity of a Beauveria bassiana Strain Isolated from Tea Weevils Myllocerinus aurolineatus Larvae FU Nanxia, LUO Zongxiu, LI Zhaoqun, BIAN Lei, XIU Chunli, CHEN Zongmao, CAI Xiaoming Chinese Journal of Biological Control    2023, 39 (5): 1104-1112.   DOI: 10.16409/j.cnki.2095-039x.2023.01.041 Abstract （119）      PDF（pc） （1236KB）（250）       Knowledge map    Save Tea weevil is an important tea insect pest in Chinese tea plantations, currently chemical pesticides are the main control method and environmentally friendly control strategies are needed urgently. To screen potential pathogenic strains for biological control of tea weevils, an entomopathogenic fungi strain isolated from field-collected infected dead Myllocerinus aurolineatus larvae was studied. Morphological characteristics and phylogenetic tree analysis revealed that the isolated strain was Beauveria bassiana (Bals.) Vuill, herein named as Maure 1.1. Under the natural pupating and hatching temperature (22℃), the laboratory pathogenicity increased with the prolonged post-inoculation observation period when M. aurolineatus larvae were exposed to B. bassiana Maure 1.1 at a concentration of 1×105―1×108 conidia/mL. When applied at the highest concentration, the Maure 1.1 strain led to 76.67% and 73.33% of cumulative corrected mortalities with immersion and soil mixing methods, respectively, at 11 days post-treatment. The LC50were 2.00×103and 1.11×103 conidia/mL, and the LT50 were 7.87 and 7.84 days for the two methods, respectively. These results indicate that the isolated B. bassiana Maure 1.1 strain is biologically pathogenic to tea weevils’ larvae and a potential candidate strain for M. aurolineatus biocontrol. Reference | Related Articles | Metrics | Comments（0） Select Optimization of Solid State Fermentation Conditions for Trichoderma harzianum BWT1.221 XIE Xiaolin, WANG Yong, CHEN Meng, ZHOU Lian, LI Chengjiang, LIU Yumin, ZHU Honghui Chinese Journal of Biological Control    2023, 39 (5): 1224-1234.   DOI: 10.16409/j.cnki.2095-039x.2023.02.049 Abstract （112）      PDF（pc） （523KB）（168）       Knowledge map    Save Trichoderma harzianum has a strong biological control effect on plant diseases. However, there are significant differences in the level of solid state fermentation among different microbial strains. In order to obtain the best optimization conditions for solid state fermentation of T. harzianum BWT1.221 which has the biocontrol function against plant pathogens and will be applied in agricultural production, single factor and orthogonal experiments were used to optimize different material composition and fermentation conditions of T. harzianum BWT1.221 solid state fermentation by measuring number of cells and the proportion of T. harzianum before and after drying in this study. The results showed that the optimum fermentation material composition of T. harzianum BWT1.221 with solid fermentation was that the mixture of bran and corn meal which were used as fermentation substrate. The optimum material propotion was about bran: corn meal = 0.25:1. The addition of sucrose as a available carbon source was 5%. The content of available nitrogen source urea and ammonium sulfate was 0.15% and 0.8%, respectively, and the ratio of material to water was 1:0.7. The results of optimal fermentation conditions for solid state fermentation showed that the fermentation temperature was 28℃, the fermentation time was 7 d, the liquid inoculum volume was 5%, and the microbial inoculum was dried at 28 ℃ for 24 h. Microbial agent was prepared by the above method, the number of cells were 10.78×109 CFU/g and the pollution rate of other microorganisms was less than 0.01%. This study provided technical support for the large-scale fermentation production of T. harzianum, which has important significance for promoting the application of biocontrol agents to control plant diseases and reduce environmental pollution. Reference | Related Articles | Metrics | Comments（0） Select Effects of Short-term Low Temperature Stress on Survival, Development and Reproduction of the Coccophagus japonicus Compere YANG Meijuan, CHEN Junyu, YE Zhengpei, FU Yueguan, WANG Jianyun, ZHU Junhong, ZHANG Fangping Chinese Journal of Biological Control    2023, 39 (5): 1029-1037.   DOI: 10.16409/j.cnki.2095-039x.2022.11.025 Abstract （110）      PDF（pc） （471KB）（224）       Knowledge map    Save This study aims to investigate the effects of short-term low-temperature stress on the survival, development and reproduction of Coccophagus japonicus Compere. Larvae and pupae of the wasps were stressed at three temperature levels, 2, 4 and 6 ℃ for 0.5, 1 and 2 d, respectively. The survival rate, developmental duration, body length, initial egg load, and female longevity of these stressed C. japonicus were assessed. Additionally, the resulting adults were exposed to 2, 4 and 6 ℃ for survival analysis. The results showed that the survival rates of larvae and pupae, the body length, the initial egg load, and the female longevity of C. japonicus were positively correlated with the temperature and negatively correlated with the duration of stress, when the wasps experienced at their larva- or pupa-stage. Also, the developmental duration was negatively correlated with the temperature and positively correlated with the duration of stress. With the decrease of temperature and the prolongation of stress time, the survival rate of larvae and pupae of C. japonicus decreased, the developmental period was prolonged, the body length was shortened, the initial egg load and the female longevity were reduced. When the adults experienced the low temperatures (2, 4 and 6 ℃), the LT50was significantly shorter than that at 27 ℃. The LT50of adults decreased with the decrease of temperature, being 4.88, 3.39 and 1.64 d at 6, 4 and 2 ℃, respectively. In conclusion, the short-term low temperature stress is detrimental to the population growth of the C. japonicus. Therefore, the annual low temperature and duration should be considered when applying this wasp in the field for control of Parasaissetia nigra Nietner. Reference | Related Articles | Metrics | Comments（0） Select Development of Compound Microbial Seed Coating Agent for the Control of Soil-borne Diseases and Underground Pest Protaetia brevitarsis in Maize Fields WANG Jiangping, WANG Yana, ZHANG Feiyan, YIN Jialu, LIU Hongwei, DONG Yaokun, LIU Qiuyue, ZHANG Liping Chinese Journal of Biological Control    2023, 39 (5): 1124-1132.   DOI: 10.16409/j.cnki.2095-039x.2023.01.009 Abstract （101）      PDF（pc） （637KB）（196）       Knowledge map    Save Microbial insecticidal seed coating agents are not available. This study aims to develop an insecticidal and disease-preventing compound microbial seed coating agent with three strains of bacillus with insecticidal and disease-preventing functions and their metabolites as the main components. The type and dosage of the auxiliaries for the seed coating agent were determined through analyses of the physicochemical properties of various additives and their influence on the growth of the 3 strains of Bacillus, which was formulated as: B. velezensis ZLP-101 (≥2×109cfu/mL), B. amyloliquefaciens ZLP-01(≥2×108cfu/mL), and B. subtilis ZLP-121 (1×108cfu/mL) mixed in a volume ratio of 1:1:1, supplemented by the film-forming agent sodium alginate 3%, the chemical synergist high efficiency cypermethrin 0.01%, the colorant acid magenta 0.2%, the thickener xanthan gum 0.1%, and the dispersant sodium lignosulfonate 2%. The agent was tested using potted maize plants in the laboratory, which showed that the efficacy of the biological seed coating agent against underground pest grubs was 70.71%, and the control efficacy of maize blight was 83.64%. The relevant indicators of this coating agent comply with the national standards for suspension seed coating agents and microbial fungal agents, which can be used for corn coating, control of soil-borne diseases and underground insect pests, and has great application potential in seed coating of other crops. Reference | Related Articles | Metrics | Comments（0） Select Effects of Pollen-added Artificial Diets on the Development of Harmonia axyridis Larvae SUN Yuanxing, CHEN Mingjuan, LI Mingling, QIN Yalong, ZHONG Mingjuan Chinese Journal of Biological Control    2023, 39 (5): 1058-1065.   DOI: 10.16409/j.cnki.2095-039x.2022.11.019 Abstract （100）      PDF（pc） （384KB）（173）       Knowledge map    Save Effective artificial diet is important for mass-rearing of predatory ladybird beetles. However, most insect-free artificial diets generally result in inferior rearing efficiency. Plant pollens are rich in nutritive and non-nutritive components and are important alternative diets for the predatory ladybird beetles under natural conditions. Until now, there are scarcely reports that pollen can be applied as a component of artificial diet. In this study, maize pollen and rose pollen were separately supplemented at three concentrations into the basic artificial diet (Control) of Harmonia axyridis, and then used to rear the 1st instar larvae the predator. The aim of this study is to evaluate the potential of pollen in improving diet quality and the impact of pollen sources and concentrations. The results showed that, when fed with pollen-added artificial diets, the H. axyridis 1st instars survived to adults at 68.33%―83.33%, which was significantly higher than that obtained with control diet (36.67%); however the total developmental duration and body weights at adult emergence were not significantly different. Pollen sources and concentrations greatly affected the development of H. axyridis larvae. Feeding on high concentration rose pollen (RS-H) and median concentration maize pollen-amended artificial diets (MZ-M) resulted in the highest survival rates of 1st instar-to-adult (83.33% and 80.00%, respectively). Our results indicate that supplementation of pollen can greatly improve the diet quality, and RS-H and MZ-M have great potential being used in mass-rearing of H. axyridis larvae. Reference | Related Articles | Metrics | Comments（0） Select The Study on Screening, Identification and Its Potential Application of Biocontrol Agents against Fusarium oxysporum f. sp. niveum XU Peiling, HAN Wenxin, WANG Gang, CHEN Wen, GAO Hongshan, Lü Minghui, YANG Xiangming, ZHU Yanze, JIANG Chunhao Chinese Journal of Biological Control    2023, 39 (5): 1253-1265.   DOI: 10.16409/j.cnki.2095-039x.2023.02.053 Abstract （100）      PDF（pc） （5693KB）（227）       Knowledge map    Save To obtain biocontrol strains with high control efficacy against watermelon Fusarium wilt, 32 strains with high chitinase activity were screened in this study, by using colloidal chitin as the only carbon source, from more than 1000 strains isolated from mangrove rhizosphere soil in Zhuhai, Guangdong province. Furthermore, 10 strains with high efficacy were obtained by using plate antagonism assay. The greenhouse results showed that the 10 strains had various growth promotion and disease prevention effects, among which the strain PL-29 treatment showed the best control efficacy of watermelon Fusarium wilt compared with the control. The control efficacy of strain PL-29 was 63.11%, and its growth promotion effect was also significant. The strain PL-29 was identified as Streptomyces flavotricini by morphological, physiological and biochemical characteristics and 16S rDNA phylogenetic analysis. In addition, the result of antagonism spectrum assay showed that the strain had antagonistic ability against Phytophthora capsici, Botrytis cinerea, Rhizoctonia solani and other pathogens. Further study on the inhibitory effect of strain PL-29 on Fusarium oxysporum showed that strain PL-29 could inhibit the formation and germination of F. oxysporum spores, and thus inhibit the growth of the pathogen. In addition, it can impair the integrity of the plasma membrane of F. oxysporum and induce ROS accumulation. These results indicate that S. flavotricini PL-29 has high potential application value in the control of watermelon Fusarium wilt. Reference | Related Articles | Metrics | Comments（0） Select Development and Population Parameters of Picromerus lewisi Scott (Hemiptera: Pentatomidae) at Different Temperatures TANG Yiting, WANG Mengqing, ZHANG Lisheng Chinese Journal of Biological Control    2023, 39 (5): 1076-1082.   DOI: 10.16409/j.cnki.2095-039x.2023.11.001 Abstract （95）      PDF（pc） （381KB）（265）       Knowledge map    Save Picromerus lewisi Scott is a predatory insect. The effects of temperature on growth and development and population parameters of P. lewisi were investigated for the purpose of improving the rearing efficiency. At the six temperatures of 18 ℃, 21 ℃, 25 ℃, 28 ℃, 32 ℃ and 36 ℃, P. lewisi could develop successfully between 18 and 32℃, while the eggs did not hatch at 36℃. The development rate of P. lewisi was linearly and positively correlated with the temperature between 18 and 32℃, which yielded an equation of Y＝0.002533X―0.03648. The degree-day requirements was 394.7 DD, and the developmental threshold temperature was 14.40 ℃. At 32 ℃, the developmental period from egg to adult was the shortest (22.40 d). The temperatures exerted no influence on weight of P. lewisi That was larger in females than in males. The results of population life table showed that the net reproductive rate was the highest at 28℃ (179.50), and the generation time was the shortest (57.50 d), which was seconded by those at 25℃ (125.64 and 64.59 d, respectively). Reference | Related Articles | Metrics | Comments（0） Select Functional Analysis of ganA in the Nematocidal of Enterobacter ludwigii against Bursaphelenchus xylophilus WANG Haoyu, ZHANG Hongtao, ZHANG Ying, ZHAO Yu, WANG Chuanzhen, ZHANG Lixia, WANG Qi, WU Di, YU Cuifang, LI Haiyong, WANG Shuang, NIU Ben Chinese Journal of Biological Control    2023, 39 (5): 1094-1103.   DOI: 10.16409/j.cnki.2095-039x.2022.11.020 Abstract （93）      PDF（pc） （1355KB）（304）       Knowledge map    Save Biocontrol microorganisms have been used to control Bursaphelenchus xylophilus disease, but the molecular mechanism of nematicidal is not clear. This study found that the corrected mortality of B. xylophilus treated with E. ludwigii AA4 was more than 85%, and strain AA4 could significantly reduce B. xylophilus infection of pine seedlings. In order to explore the molecular mechanism of AA4 against B. xylophilus, the DNA homologous recombination technique was used to construct the β-galactosidase gene ganA, and the function of ganA in AA4 nematode inhibition was analyzed. The results showed that the B. xylophilus killing activity and B. xylophilus disease prevention in a ganA knockout mutant strain decreased significantly, and the mobility and ability to colonize nematodes of the mutant strain decreased significantly. In addition, compared with the wild-type strain, the ability of the mutant strain to restrict the movement of nematodes was significantly reduced. Therefore, ganA may participate in the process of AA4 killing B. xylophilus by regulating AA4’s ability to colonize B. xylophilus and restrict nematode movement. Reference | Related Articles | Metrics | Comments（0） Select Analysis of Inhibitiory Active Substances of Penicillium bilaiae Strain 47M-1 Based on Non-Targeted Metabolomics ZHAO Xinbei, NI Yunxia, LIU Xintao, ZHAO Hui, YAN Wenqing, HE Bipo, LIU Hongyan Chinese Journal of Biological Control    2023, 39 (5): 1156-1171.   DOI: 10.16409/j.cnki.2095-039x.2023.02.055 Abstract （93）      PDF（pc） （1605KB）（585）       Knowledge map    Save Strain 47M-1 of Penicillium bilaiae showed significant inhibitory effect on a variety of sesame pathogenic fungi. In order to analyze the inhibitiory active substances produced by this strain, liquid chromatography tandem mass spectrometry was used to detect the metabolites of strain cultured for 48 h, 96 h and 144 h. Then, classification of up-regulated differential metabolites in the HMDB database and enrichment of KEGG metabolic pathways were analyzed in order to identify the active metabolites. A total of 105 up-regulated differential metabolites were detected, which were mainly enriched in the superclasses of phenylpropanoids and polyketides. The enrichment analysis of the KEGG metabolic pathways showed that the up-regulated differential metabolites were mainly enriched in metabolic pathways, biosynthesis of cofactors, purine metabolism, biosynthesis of amino acids and ABC transporters. The inhibitiory activities of some up-regulated differential metabolites were verified using medicated plate method. These compounds naringenin, protocatechuic acid, D-(-)-quinic acid, hesperetin, 3-(3-Hydroxyphenyl) propanoic acid, 3, 4-dihydroxyhydrocinnamic acid, 5, 7-dihydroxy-2-(4-hydroxyphenyl)-3, 4-dihydro-2H-1-benzopyran-4-one, rosmarinic acid, bergapten and eriodictyol all showed excellent inhibition activities against sesame pathogenic fungi. In this study, the major groups and key metabolic pathways of potential inhibition metabolites produced by strain 47M-1 were identified, and the inhibitiory activity of some up-regulated differential metabolites was verified. This study will provide data support for the further development and utilization of strain 47M-1 and the research and development of microbial pesticide. Reference | Related Articles | Metrics | Comments（0） Select Prevention and Control Effect of Two Bacillus Strains on Soil-borne Phytophthora capsici in Seedling Stage ZHU Wenhui, QIN Pu, ZHAO Yan, YIN Hui, REN Lu, ZHOU Jianbo Chinese Journal of Biological Control    2023, 39 (5): 1235-1243.   DOI: 10.16409/j.cnki.2095-039x.2023.02.051 Abstract （93）      PDF（pc） （1162KB）（171）       Knowledge map    Save Transplanting seedlings with pathogen results in the spread of pepper blight. In order to effectively use biocontrol bacterium to prevent and control pepper blight at seedling stage,the efficacy of Bacillus velezensis LYK10 and Bacillus cereus QTK2 against pepper blight of seedling by soaking seed were tested by point plate seedling with biocontrol bacteria. The synergies of mixed biocontrol bacterium and biocontrol bacteria combined chemical fungicide were mensurated. The results showed that LYK10 and QTK2 could delay the occurrence time of pepper seedling disease, and the control efficiency of pepper seedling disease was respectively 30.55% and 33.33%, and the emergence rate was increased by 1.39% and 2.78% in turn. The both bacterium had antagonistic effects on Phytophthora capsici., and also induced the changes of CAT and PPO enzyme activity in pepper plants. The efficiency of the two mixed bacterium at equal concentration improved from 27.78% to 42.45% compared with the two single bacteria treatments. The efficiency of the mixture of LYK10 and dienomorph with the consistence of 5.0×107cfu/mL+300 µg/mL and 5.0×106cfu/mL+150 µg/mL respectively were 48.15% and 38.89%. The control effect of high concentration bacterial-drug treatment was higher than the treatments of 108 cfu/mL LYK10 and 300 g/mL dienomorph. The control effect of low concentration bacterial-drug treatment was higher than LYK10 and comparable to dienomorph. The results can provide data to support for the research on biological control to pepper blight and effective utilization of biocontrol bacterium. Reference | Related Articles | Metrics | Comments（0） Select Preparation of Biocontrol Agent for Bacillus velezensis ZJ1 Oil Suspension and their Plant Growth-promoting and Biocontrol Properties in Field ZOU Xiaolu, ZHAO Yu, MA Dongli, ZHAO Xiaojun, YIN hui, REN Lu Chinese Journal of Biological Control    2023, 39 (5): 1204-1214.   DOI: 10.16409/j.cnki.2095-039x.2023.02.047 Abstract （92）      PDF（pc） （1516KB）（196）       Knowledge map    Save A strain of Bacillus velezensis ZJ1 with good inhibitory efficiency against tomato early blight and gray mold caused by Alternaria solani and Botrytis cinerea was accquired by using the dual culture. The freeze drying method and biocompatibility test were used to select the optimal formula of bacterial agent, the field efficacy and the growth-promoting effect of ZJ1 oil suspension was determined. The optimum formulation of ZJ1 strain oil suspension was 10% bacteria powder (6.0×1010cfu/g), 7% (v/v) Tween80, 50 μg/mL amphotericin, 2% CaCl2, 1% SDS, 1% CaCO3, 0.5% calcium lentisulfonate, 5% dextrin, peanut oil to complement to 100%, the spores content is 5.75×109cfu/mL. All indexes were in line with national standards. The B.velezensis ZJ1 oil suspension with the dilution of 200× had the best biocontrol effect on tomato early blight and gray mold caused by A.solani and B.cinerea, the protective and therapeutic effects can reach more than 90%, and has obvious growth-promoting effect, and could significantly increase the stem diameter. The preparation of this biocontrol agent is environmentally friendly and highly-efficient, and has stable effect, which lays a foundation for its production and application in fields. Reference | Related Articles | Metrics | Comments（0） Select Biological Activities of Seven Plant Essential Oils against Panonychus ulmi and GC-MS Analysis of Peppermint Essential Oil Components ZHANG Kun, WANG Kaile, ZHANG Pengjiu, LIU Zhongfang, YANG Jing, FAN Jianbin, ZHAO Jinyu, FAN Renjun, GAO Yue Chinese Journal of Biological Control    2023, 39 (5): 1133-1142.   DOI: 10.16409/j.cnki.2095-039x.2023.01.037 Abstract （92）      PDF（pc） （437KB）（217）       Knowledge map    Save In order to screen new and green plant-derived acaricides, seven plant essential oils were extracted by steam distillation. The contact killing, fumigation and repellent activities of the essential oils against Panonychus ulmi were determined by Potter spray method, fumigation method and medicine film method. The peppermint essential oil with strong biological activity was screened out, and the chemical components of the peppermint essential oil were identified and analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that the LC50 of the peppermint essential oil at 12, 24, and 48 h post spray were 2.13, 0.83, and 0.77 g/L, respectively. The LC50 at 12, 24, and 48 h post fumigation were 382.86, 73.89, and 16.08 μL/L, respectively. The results indicate that the essential oil has strong contact killing and fumigation activities against P. ulmi. Finally, at 24 h post repellent treatment, the peppermint essential oil repelled 77.82% of P. ulmi, which was at grade IV. The peppermint essential oil was analyzed by GC-MS, and a total of 34 chemical components were identified. The detected chemical components accounted for 99.67% of the total essential oil content. The main components were menthol (32.16%), limonene (16.44%), 4-isopropyltoluene (11.32%), 3-carene (10.27%), diethyl malonate (9.57%) and menthone (3.91%). These results show that the peppermint essential oil has strong biological activity against P. ulmi, which provides a scientific basis for the further development of plant-derived acaricides. Reference | Related Articles | Metrics | Comments（0） Select Research of Biocontrol Actinomycete ZZ-9 Granules and Its effect on cucumber Rhizoctonia Rot ZHANG Yang, WEI Lanjing, CHEN Junhong, HEI Yaya, XUE Yingyu Chinese Journal of Biological Control    2023, 39 (5): 1194-1203.   DOI: 10.16409/j.cnki.2095-039x.2023.02.044 Abstract （91）      PDF（pc） （446KB）（177）       Knowledge map    Save Streptomyces rochei ZZ-9 is a biocontrol strain that with broad-spectrum antifungal activity. The mycelium growth rate method was utilized in this work to screen carriers, stabilizers, disintegrants, adhesives, and wetting agents. The optimal formula of Streptomyces rochei ZZ-9 granules were: the ratio of bacterial solution and attapulgite was 2:5, calcium carbonate 1%, magnesium chloride 2%, polyvinyl alcohol 1%, sodium dodecylbenzene sulfonate 0.5%. The effective viable number of ZZ-9, particle size, moisture content, disintegration time in water, pH value and hardness were 1.19×108cfu/g, 93.5%, 2.5%, 96 s, 6.35 and 10%. The results of pot experiment showed that the optimal controlling effect of cucumber Rhizoctonia rot was 76.92% after application of ZZ-9 granule with 3 g/pot. In addition，the Streptomyces rochei ZZ-9 granules presented significant promoting effects in pot experiment. Compared with the control, the germination rate, plant height, root length and fresh weight of cucumber seedlings were increased by 9.34%, 29.52%, 27.54% and 22.96% after application of Streptomyces rochei ZZ-9 granules, respectively. Reference | Related Articles | Metrics | Comments（0） Select Screening and Identification of Biocontrol Potential Myxobacteria Strains against Fire Blight and Pear Canker Diseases and Evaluation of Indoor Control Efficacy BAI Xueying, HAN Jian, SUN Boyuan, SONG Shumin, LUO Ming, SUN Liying Chinese Journal of Biological Control    2023, 39 (6): 1384-1397.   DOI: 10.16409/j.cnki.2095-039x.2023.02.059 Abstract （90）      PDF（pc） （3040KB）（311）       Knowledge map    Save Fire blight causted by Erwinia amylovora and pear canker causted b Valsa pyri are two major diseases affecting the production of Pyrus sinkiangensis. This study aimed to develop biological control agent for combating fire blight and pear canker diseases by utilizing myxobacteria strains isolated from soil samples collected from different prefectures in Xinjiang. The predation efficiency of myxobacteria strains on E. amylovora was compared by using bacterial lawn co-culture, while the inhibitory effect of myxobacteria strains on hyphal growth and conidial germination of V. pyri was assessed by the pair-culuturing method. Myxobacteria strains with significant predation efficacy and antagonistic activities against E. amylovora and V. pyri were screened, and the control effects of the strains on these two diseases were further evaluted by detached leaves, branches and greenhouse potted experiments. The myxobacteria strains with biocontrol potential were identified based on morphological characteristics, 16S rRNA and lep A gene sequence homology analysis. The results showed that the six selected myxobacteria strains exhibited substantial predation effects on E. amylovora, demonstrated by significant reduction of the bacterial numbers from 109 to 102～105 cfu/mL. Additionally, these strains demonstrated strong antagonistic activity against V. pyri, with inhibition rates of mycelial growth and conidial germination over 70% and 87%, respectively. Strains NST12 and NST47 showed protective and therapeutic effects of over 75% against fire blight on detached P. sinkiangensis leaves and P. betulifolia seedlings, while strains NST47 and NST49 exhibited protective and therapeutic effects of over 72% and 54% against pear canker on detached twigs, respectively. Notably, strain NST47 displayed remarkable biocontrol potential against both fire blight and pear canker. Based on the morphological and molecular identification results, strains NST49 and NST12 were identjfied as Myxococcus fulvus and NST47 was identjfied as Archangium violaceum. Reference | Related Articles | Metrics | Comments（0） Select Effects of Plant Secondary Metabolite DIMBOA on Therioaphis trifolii Monel (Homptera: Aphididae) PAN Fan, ZHU Kaihui, TU Xiongbing, ZHANG Zehua Chinese Journal of Biological Control    2023, 39 (5): 1143-1147.   DOI: 10.16409/j.cnki.2095-039x.2023.01.014 Abstract （88）      PDF（pc） （396KB）（265）       Knowledge map    Save The effects of plant secondary metabolite DIMBOA on the growth, development and reproduction of Therioaphis trifolii Monel were investigated by mixingdifferent concentrations of DIMBOA with artificial diet. The results showed that the survival rate, reproductive rate and protease activity of T. trifolii were significantly reduced by high concentration of DIMBOA. When the T. trifolii adults were fed 800 μg/mL DIMBOA for 72 h, compared with the control group, the survival rate was reduced by 53.06% and the reproductive rate, by 53.82%. The survival rate of the T. trifolii larva was only 35.8%, significantly lower than that of the control group. The activities of aminopeptidases (APs), chymotrypsin (CTP), trypsin (Tr), and total protease (Pr) in T. trifolii adults were significantly inhibited by different concentrations of DIMBOA, especially by high concentration of DIMBOA. The activities of Aps, CTP, Tr and Pr were reduced by 61.5%, 67%, 65.1% and 55.9%, respectively, in the insects fed with 800 μg/mL DIMBOA for 72 h. These results indicate that DIMBOA can significantly reduce the survival rate and reproductive rate of T. trifolii, showing a potential for the development of plant-derived pesticides. Reference | Related Articles | Metrics | Comments（0）