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Published in last 1 year |  In last 2 years |  In last 3 years |  All Please wait a minute... For Selected: Download Citations EndNote Ris BibTeX Toggle Thumbnails Select Mechanism of Chaetomium globosum in Promoting Growth and Preventing Disease and Advance of Its Application and Research FENG Chaohong, LI Lijuan, ZHANG Jiaojiao, WANG Junmei, SONG Yuli, LI Haohai, XU Fei Chinese Journal of Biological Control    2023, 39 (4): 961-969.   DOI: 10.16409/j.cnki.2095-039x.2023.02.043 Abstract （452）      PDF（pc） （432KB）（6056）       Knowledge map    Save Chaetomium globosum, one of common plant endophytic fungi, possesses potential biocontrol function against many plant diseases. This paper summarized the research advances of Chaetomium globosum on biocontrol effect, and its biocontrol mechanisms, which include antibiosis, growth-promoting effect, induction of plant resistance, niche and nutrient competition, and hyperparasitism. Chaetomium globosum exhibits great application potential on plant disease control and growth promotion, soil improvement, and straw degradation. Advances on the fermentation technology and genetic engineering were also illuminated. This study will provide theoretical references for the application research of Chaetomium globosum as biocontrol agent. Reference | Related Articles | Metrics | Comments（0） Select Effects and Colonization of Beauveria bassiana in Maize under Stress of Exserohilum turcicum Infection SUI Li, LU Yang, CHI Ruikai, ZHAO Yu, ZHANG Zhengkun, LI Qiyun Chinese Journal of Biological Control    2023, 39 (4): 804-812.   DOI: 10.16409/j.cnki.2095-039x.2022.05.008 Abstract （358）      PDF（pc） （1371KB）（1406）       Knowledge map    Save This study aims to clarify the colonization ability of Beauveria bassiana in maize plants and its influence on disease resistance of host plants. B. bassiana-maize symbiosis was constructed by root drenching method and chlorophyll and nitrogen content, as well as plant height were measured to evaluate the growth promoting effect of B. bassiana colonization in maize. The resistance of B. bassiana colonization against Exserohilum turcicum infection was investigated by inoculation of the disease. The colonization and spread of B. bassiana endophytic in maize plants under E. turcicum infection stress was studied by colony counting and quantitative PCR. The results showed that the colonization of B. bassiana in maize reached 68.18%, positively promoted plant growth and significantly reduced the incidence of B. bassiana by 46.67% at 21 day after inoculation. B. bassiana could be 'recruited' from the rhizosphere soil when maize leaves were infected by E. turcicum, and B. bassiana could accumulate at leaves in turn to improve resistance against plant pathogen infection. This study has further clarified the biocontrol potential of entomogenous fungi against plant diseases as endophyte, and provides a theoretical basis for the study of colonization and induced resistance of entomogenous fungi in plant tissues. Reference | Related Articles | Metrics | Comments（0） Select Visual System and Opsin in Insect TANG Yanhong, BI Siyan, WANG Xiaodi, MA Dongfang, YANG Nianwan, GUO Jianyang, LÜ Zhichuang, LIU Wanxue Chinese Journal of Biological Control    2023, 39 (3): 718-730.   DOI: 10.16409/j.cnki.2095-039x.2023.05.004 Abstract （357）      PDF（pc） （1851KB）（6327）       Knowledge map    Save Insects have a well-developed and sensitive visual system, and opsin is a basic component of animal vision, and its structure largely determines the sensitivity of visual pigments to light of different wavelengths. The duplication and differentiation of opsin genes are the main mechanisms for the production of new light pigment sensitivity. Throughout the evolution of animals, many instances of opsin gene duplication and loss have occurred, forming the sensitivity of the various animal visual systems we observe today. In recent years, extensive and in-depth research has been carried out on visual proteins. This article describes the types of photoreceptors, the color vision system of insects, the classification of visual proteins, the phenomenon of co-expression of visual proteins, the duplication and deletion of visual protein genes, and the mechanism and research status of the physiological functions of visual proteins. Reference | Related Articles | Metrics | Comments（0） Select Research Progress on Ecological Regulation to Crop Pests using Agricultural Biodiversity YAN Xueying, WANG Hui, ZHANG Yanjun, ZHAO Jianning, YANG Dianlin, ZHANG Guilong Chinese Journal of Biological Control    2023, 39 (3): 710-717.   DOI: 10.16409/j.cnki.2095-039x.2023.05.003 Abstract （347）      PDF（pc） （393KB）（5646）       Knowledge map    Save At present, in the context of protecting biodiversity, maintaining the sustainable and healthy development of ecosystems and reducing the use of fertilizers and pesticides, using agricultural biodiversity to control pests has become one of the hotspots in domestic and foreign research. Agricultural biodiversity is important for maintaining the function of agricultural ecosystem and the control of crop pests are of great significance. This paper briefly describes the concept and current situation of agricultural biodiversity, and summarizes the scientific principles or theories of pest ecological regulation of biodiversity, starting from the protection of agricultural biodiversity by non-agricultural habitats and its influencing factors on agricultural biodiversity. The methods and specific applications of ecological regulation to crop pests using agricultural biodiversity are analyzed at the three levels of farmland, farmland boundary and farmland landscape pattern, and finally put forward the development direction of ecological regulation to crop pests using agricultural biodiversity, which provides knowledge, principles, methods and technical support for effective pest ecological regulation. Reference | Related Articles | Metrics | Comments（0） Select The Characteristic of Plant Diseases and Insect Pests in Greenhouse Horticulture and Its Green Control Strategy of China WU Shengyong, ZHANG Mengdi, XU Jin, WANG Endong, CUI Li, LEI Zhongren, SHI Xiaobin, XIE Xuewen, WANG Shaoli, WANG Su, WEI Shujun, MA Zhongzheng, HE Limei, GAO Yulin Chinese Journal of Biological Control    2024, 40 (5): 1169-1180.   DOI: 10.16409/j.cnki.2095-039x.2022.01.022 Abstract （338）      PDF（pc） （476KB）（1501）       Knowledge map    Save Greenhouse horticultures has been rapid development in China, playing an important role in promotion agricultural modernization and rural revitalization. Based on the current trajectory of the horticulture industry in China, we first summarized the characteristic of plant diseases and insect pests and its control strategies, including the agricultural control, physical control, biological control, ecological regulation, biotechnology and science-based application of chemical pesticides. Finally, we put provide suggestions on the control of horticultural diseases and insect pests under protected conditions, providing baseline references for the sustainable development of horticultural industry. Reference | Related Articles | Metrics | Comments（0） Select Virulence of Entomopathogenic Nematodes to Holotrichia parallela Larvae ZHANG Cailing, WANG Zhimin, WANG Siyu, Ibrahim Osman, LI Kebin, ZHANG Shuai, CAO Yazhong, WANG Senshan, YIN Jiao Chinese Journal of Biological Control    2024, 40 (5): 1009-1014.   DOI: 10.16409/j.cnki.2095-039x.2022.03.025 Abstract （301）      PDF（pc） （361KB）（575）       Knowledge map    Save The pathogenicity of seven entomopathogenic nematodes (EPNs) against the late 1st instar larvae of Holotrichia parallela was tested in laboratory to screen the effective strains of entomopathogenic nematodes for the H. parallela control, and the most suitable environmental conditions of strains were identified. Results showed that pathogenicity of seven EPNS strains were different under laboratory conditions, the Heterorhabditis bacteriophora (D26-6) had the strongest pathogenicity to the larvae of H. parallela. In addition, temperature, soil moisture and dosage of EPNs all take effects on the pathogenicity. The optimum temperature and soil moisture for H. bacteriophora (D26-6) infection were 25 ℃ and 18% respectively, when the ratio of H. parallela larvae to D26-6 nematodes was 1:100, the mortality of H. parallela larvae can reach 100% after 11 days. Reference | Related Articles | Metrics | Comments（0） Select Research Progress on Biology, Ecology and Biological Control of Cheilomenes sexmaculata (Coleoptera: Coccinellidae) TANG Liangde, ZANG Liansheng Chinese Journal of Biological Control    2023, 39 (3): 697-709.   DOI: 10.16409/j.cnki.2095-039x.2023.05.002 Abstract （298）      PDF（pc） （467KB）（3282）       Knowledge map    Save The ladybeetle Cheilomenes sexmaculata (Fabricius) (Coleoptea: Coccinellidae) is one of the dominant natural enemies of various insect pests in agroforestry ecosystem due to wide distribution, common occurrence in field, high reproductive rate, wide feeding range, excellent predation ability and strong adaptability, thus there is a bright development prospect to develop this predator. In this article we reviewed the research progress on C. sexmaculata in recent 40 years, mainly focusing on the taxonomy, biological characteristics, biological control, artificial rearing, and insecticide impacts. In addition, the prospects for the future studies and application of C. sexmaculata were also discussed. Reference | Related Articles | Metrics | Comments（0） Select Review and Prospects on Microbiological Pesticides Used on Grain Crops in China ZHUO Fuyan, ZHANG Hongjun, LIU Wancai, ZHANG Jie Chinese Journal of Biological Control    2023, 39 (4): 747-751.   DOI: 10.16409/j.cnki.2095-039x.2023.09.001 Abstract （287）      PDF（pc） （334KB）（638）       Knowledge map    Save Microbial pesticides are an important way to promote the green control of crop pests and diseases, and also an effective way to ensure increase production and reduction loss of grain crops. Based on the application acreage of microbial pesticides on grain crops, this paper reviewed the achievements of microbial pesticide application since 2018 in view of crops types and application regions. This paper also discussed and sorted out the existing problems with microbial pesticide application, and proposed the corresponding promotion ideas. The review serves to provide reference for the development of microbiological pesticide. Reference | Related Articles | Metrics | Comments（0） Select The First Report of a New Parasitic Wasp Phaedrotoma sp.2 Parasitizing Liriomyza trifolii in Hainan XING Binglin, GULINUER·Ahamaijiang, WU Shaoying, PENG Zhengqiang, YANG Lei, LÜ Baoqian Chinese Journal of Biological Control    2023, 39 (3): 740-746.   DOI: 10.16409/j.cnki.2095-039x.2022.03.029 Abstract （286）      PDF（pc） （5611KB）（1009）       Knowledge map    Save Liriomyza trifoliata is one of the major invasive pests in China and is mainly distributed in tropical and subtropical areas, causing huge losses. Parasitic wasps, a large group of hymenopteran insects, play an important role in the biological control of pests. Based on this point, a general survey of wasps parasitizing L. trifoliata was carried out in Hainan Province from October to December 2021. A new species that has not been reported in China was identified by morphological identification, which belongs to the genus of Phaedrotoma (Hymenoptera: Braconidae), it was named as Phaedrotoma sp.2. Molecular identification based on the mitochondrial COI gene further confirmed the taxonomic status of the wasp, and the phylogenetic tree showed that it was clustered into the genus of Phaedrotoma. This study lays a foundation for the artificial breeding of L. trifoliata parasitoids in the future and contributes to the application of biological control of agricultural pests. Reference | Related Articles | Metrics | Comments（0） Select Parasitism Ability and Offspring Development of Sclerodermus alternatusi Yang (Hymenoptera: Bethylidae) in Different Female Oviposition Times TANG Yanlong, WANG Lina, CHEN Yan, KANG Kui, ZENG Boping, YANG Zhongqi, WEI Ke Chinese Journal of Biological Control    2023, 39 (3): 499-506.   DOI: 10.16409/j.cnki.2095-039x.2022.11.004 Abstract （283）      PDF（pc） （484KB）（869）       Knowledge map    Save Sclerodermus alternatusi is a synovigenic parasitoid in which oogenesis takes place after the female is stimulated by feeding on a host, and this is a successive process. Generally, one female parasitoid can complete several times of parasitism and thus parasitize more than one hosts in its life span. In order to compare the differences of adult parasitism ability and offspring development of S. alternatusi in different adult oviposition times, the female parasitism rate, successful parasitism rate, progeny developmental duration, brood size, and male proportion were investigated in the laboratory conditions. The results showed that S. alternatusi female could complete four times of parasitization to the host, Thyestilla gebleri larvae. There were 85% of female parasitoids could complete two times of parasitization, but only 31% and 4% of females could complete three or four times of parasitization. The parasitic ability of the female parasitoid gradually decreases with the increase of the oviposition times. The durations of egg stage showed no significant difference in different oviposition times. However, the larval stage and pupal stage of the offspring were significantly different in different oviposition times, which displaying the longest in first oviposition time and the shortest in fourth oviposition time. The numbers of female and male offspring were significantly different among different oviposition times. The female offspring numbers were 40.2 and 35.1 in the first and second oviposition time, respectively. The numbers of male offspring were also less than 10 in these two oviposition times. The numbers of female offspring were sharply declined in the third oviposition time, over 60% of offsprings were males. Moreover, all of the offspring were males in the fourth oviposition time. The mean number of female offspring of a single S. alternatusi adult was approximately 80. This study provides new references for rearing and using the S. alternatusi to control the longhorn beetles. Reference | Related Articles | Metrics | Comments（0） Select Trapping Synergism of E8, E10-12:OH with Sex Pheromone of Grapholita molesta (Busck) (Lepidoptera: Tortricidae) ZHU Mengya, XIANG Huiming, LI Xianwei, WU Dongsheng, WU Hongjian, MA Ruiyan Chinese Journal of Biological Control    2023, 39 (3): 560-566.   DOI: 10.16409/j.cnki.2095-039x.2022.03.032 Abstract （275）      PDF（pc） （412KB）（770）       Knowledge map    Save The aim of this paper was to clarify the synergistic effect of different doses of E8, E10-12:OH that is the main component of sex pheromone of Cydia pomonella on the sex pheromone of Grapholita molesta, and to provide a reference for biological control and monitoring of G. molesta. Field trapping experiments and electroantennogram (EAG) were used to explore the effect of lures, which contained different dosages of E8, E10-12:OH mixing with the sex pheromone of G. molesta, on trapping efficiency of G. molesta in the field and on the EAG responses of male G. molesta. Field experiment showed that all five doses of E8, E10-12:OH used alone had a capability to trap male G. molesta and had no significant difference in trapping number of male G. molesta. Trapping efficiency of five doses of E8, E10-12:OH mixing with G. molesta sex pheromone were higher than it. The number of G. molesta captured by adding 20 μg of E8, E10-12:OH to the sex pheromone of G. molesta was 2.86 times higher than it. Both E8, E10-12:OH and G. molesta sex pheromone could elicit the EAG responses of male G. molesta, of which the intensity was positively related with the concentration of tested compounds. E8, E10-12:OH could be used as attractant or synergist of the sex pheromone of G. molesta to improve its trapping efficiency and enhance the accuracy of monitoring the population dynamics of G. molesta. Reference | Related Articles | Metrics | Comments（0） Select Mrhox3 Effects on Conidiation and Tolerance in Metarhizium robertsii ZHANG Nan, WU Hao, TONG Youmin, LI Xiaojuan, HUANG Bo Chinese Journal of Biological Control    2023, 39 (3): 523-532.   DOI: 10.16409/j.cnki.2095-039x.2022.03.019 Abstract （271）      PDF（pc） （848KB）（613）       Knowledge map    Save Metarhizium robertsii, as a model entomopathogenic fungus, has been applied in biocontrol programs of insect pests. Homeobox family genes play an important role in fungal asexual reproduction, however, its functions in entomopathogenic fungi remains unclear. Quantitative real-time PCR showed that homeobox family gene Mrhox3 is highly expressed at the conidiation and conidia stages. Gene knock-out and complementation were conducted to study the function of Mrhox3, and the results indicated that deletion of Mrhox3 did not affect the mutant’s vegetative growth and pathogenicity, but significantly weakened the conidiation ability. Further studies showed that loss of Mrhox3 significantly down-regulated the expression levels of multiple conidiation -related genes, such as the centarl regulators abaA and wetA. Loss of Mrhox3 resulted in promoted conidial germination and significantly enhanced conidial tolerance to UV and heat shock stress. This study provides a foundation for further understanding the mechanism of conidiation and conidia stress resistance in Metarhizium robertsii. Reference | Related Articles | Metrics | Comments（0） Select Intraspecific Host Discrimination and Superparasitism in Telenomus remus (Nixon), an Egg Parasitoid of Spodoptera frugiperda (J. E. Smith) XUE Taoran, PAN Jing, LIU Lihong, XU Teng, HUANG Jian, HE Yuxian, WANG Zhuhong Chinese Journal of Biological Control    2024, 40 (1): 1-.   DOI: 10.16409/j.cnki.2095-039x.2023.01.026 Abstract （271）      PDF（pc） （471KB）（973）       Knowledge map    Save Telenomus remus (Nixon) is an important egg parasitoid of Spodoptera frugiperda (J. E. Smith), a major invasive pest. In order to clarify the intraspecific host discrimination and the superparasitism of T. remus, the self-recognition (same individual) and conspecific-recognition (different individual) ability to recognize the hosts eggs parasitized at different time intervals were determined, and the superparasitism rate of single and multi-female T. remus under different host density were also investigated. The results showed no superparasitism in T. remus , either self-recognition or conspecific-recognition. After a single female T. remus parasitizing for 24 h at different parasitoid-egg ratios, the percentage of 1st instar larvae of T. remus in S. frugiperda eggs was less than 100%, and only one was found in each host egg. With multi-female T. remus and sufficient hosts, and when parasitoid-egg ratios were 1:100, 2:200, 4:400, 6:600 and 8:800, the percentage of unparasitized host eggs were 52.8%, 67.2%, 63.2%, 53.6% and 51.2%, respectively, and the percentage of host eggs with one 1st instar larva were 47.2%, 32.8%, 36.8%,46.4% and 48.8%, respectively. With multi-female T. remus and insufficient hosts, and when parasitoid-egg ratios were 2:20, 3:21, 4:20, 7:21 and 20:20, the parasitism rates of T. remus were 60.0%, 83.0%, 95.0%, 94.0% and 96.0%, respectively, and the superparasitism rates were 11.0%, 38.0%, 64.0%, 73.0% and 92.0%, respectively, the average number of 1st instar larva of T. remus in a single host egg were 0.4, 0.6, 1.1, 1.3 and 2.1, respectively, and the maximum number were 3.0, 4.0, 5.0, 6.0 and 12.0, respectively. The results show that T. remus has the ability of self and conspecific-recognition, the superparasitism of a single female T. remus does not depend on host density. When the host is sufficient, the superparasitism of multi-female T. remus does not occur, but when the host is insufficient, the parasitism and superparasitism rate, the average and maximum number of 1st instar larvae in a single host egg increase with the increase of parasitoid-egg ratios. The parasitoid-egg ratio of 4:20 is suitable for propagation of the wasp because it produces high parasitism rate, low superparasitism rate, and low internal competition. Related Articles | Metrics | Comments（0） Select Advances in Biological Control of Tuta absoluta (Meyrick) Based on Predatory Natural Enemy Resources LUO Taotao, YAN Shuyan, CAO Mengyu, ZHANG Jinghang, ZHANG Guifen, WAN Fanghao, ZHANG Ya, LI Tao, LIU Shuangqing, ZHANG Yibo Chinese Journal of Biological Control    2024, 40 (4): 727-738.   DOI: 10.16409/j.cnki.2095-039x.2024.02.039 Abstract （266）      PDF（pc） （432KB）（2178）       Knowledge map    Save Tuta absoluta (Meyrick) is a major invasive alien species worldwide, which mainly damages tomato crops by mining leaves and fruits. Since its invasion in Yili, Xinjiang in 2017, it has spread to many provinces (municipalities and autonomous regions) in China, with outbreaks in some places, seriously threatening the healthy production of China's tomato industry. At present, biological control based on predatory natural enemies is one of the main measures for integrated management of T. absoluta. So far, few review papers have been published on the predatory natural enemy resources of tomato leaf miner. Therefore, this paper systematically sorted out the species of predatory natural enemies of T. absoluta worldwide, summarized the predatory ability of predatory natural enemies of tomato leaf miner, the life parameters of predatory natural enemies of T. absoluta, and the effects of chemical pesticides on the prevention and control of T. absoluta by predatory natural enemies. The existing problems and development direction of T. absoluta control by predatory natural enemies were analyzed in order to provide reference for the biological control of T. absoluta in China. Reference | Related Articles | Metrics | Comments（0） Select Molecular Mechanism of Azadirachtin Inhibiting Trehalase Activity Leading to Abnormal Molting and Metamorphosis of Helicoverpa armigera Larvae CHANG Yanpeng, ZHANG Bo, GENG Zichen, WEI Jizhen, AN Shiheng, ZHAO Wenli Chinese Journal of Biological Control    2023, 39 (3): 550-559.   DOI: 10.16409/j.cnki.2095-039x.2022.03.015 Abstract （256）      PDF（pc） （1462KB）（978）       Knowledge map    Save Azadirachtin is a botanical pesticide with good control effect on Lepidoptera, such as Helicoverpa armigera. In this study, the molecular mechanism of the death of H. armigera larvae after feeding with Azadirachtin was analyzed by the laboratory virulence assay, real-time quantitative PCR (qRT-PCR), and metabolite measurement. Firstly, the phenotype, body weight, mortality and pupation rate of the 5th and 6th instar larvae which were fed with different concentrations of Azadirachtin were analyzed. The results showed that Azadirachtin resulted in decreased body weight, increased mortality and decreased pupation rate of H. armigera larvae. Furthermore, we proved that Azadirachtin inhibiting the biosynthesis and hydrolysis of trehalose, the transcripts of 20E downstream genes, and reducing the ATP content through by qRT-PCR, enzyme activity and endogenous substance contents measurement. The results of this study indicate that Azadirachtin inhibited the biosynthesis and metabolism of trehalose, decreased the transcripts of 20E pathway genes, and reduced ATP production in H. armigera, so that causing molting and metamorphosis failure, and eventually led to the death of the larvae. This study is of great significance for further exploring the mechanism of Azadirachtin-induced death in H.armigera and control of pests by Azadirachtin. Reference | Related Articles | Metrics | Comments（0） Select Predatory Responses of Cheilomenes sexmaculata to Diaphorina citri HE Wancai, YE Qingqing, WANG Feifeng, HU Yuwei, LIU Jinhua, MAO Xiongxing, ZHANG Ruifeng, WANG Xingmin, QIU Baoli, LIU Yutao, SANG Wen Chinese Journal of Biological Control    2023, 39 (3): 514-522.   DOI: 10.16409/j.cnki.2095-039x.2023.05.001 Abstract （256）      PDF（pc） （449KB）（818）       Knowledge map    Save Diaphorina citri Kuwayama is an important pest of citrus, navel oranges, and other members of Rutaceae family. Cheilomenes sexmaculata (Fabricius) is a common insect natural enemy in southern China. To assess the potential of C. sexmaculata to manage D. citri, the predatory functional response, search efficacy, and self-density interference response of the larvae (1st and 4th instar) and adults against various life stages of D. citri (early, late instar nymphs, and adults) were examined. Our findings demonstrated that the predatory functional response of Holling II is present in C. sexmaculata larvae and adults. The maximum daily predation amounts of the 1st instar larvae of C. sexmaculata against the early and late instar nymphs of D. citri was 55.55 and 66.66 individuals, respectively. The maximum daily predation amounts of the 4thinstar larvae of C. sexmaculata against the early, late instar nymphs and adults of D. citri was 500, 200 and 200 individuals, respectively. The maximum daily predation amount of the C. sexmaculata adults against early, late instar nymphs and adults of D. citri was 333.33, 250 and 125 individuals, respectively. With an increase in prey density, C. sexmaculata's ability to find D. citri reduced. With a rise in interference from the predator’s density at a constant D. citri density, C. sexmaculata’s predation effectiveness fell while its sharing competition intensity increased. This study offers a theoretical foundation for employing C. sexmaculata to control D. citri and Huanglongbing in the field. Reference | Related Articles | Metrics | Comments（0） Select Screening and Biocontrol Characteristics of Endophytic Strains against Ginger Bacterial Wilt DANG Keke, PENG Junwei, SUN Yang, XIE Ziyu, DONG Yuanhua, LI Jiangang Chinese Journal of Biological Control    2023, 39 (3): 585-593.   DOI: 10.16409/j.cnki.2095-039x.2023.02.030 Abstract （252）      PDF（pc） （616KB）（1925）       Knowledge map    Save Ginger bacterial wilt is a soil borne bacterial disease caused by Ralstonia solanacearum, which seriously restricts the development of ginger industry. In this study, endobacteria from ginger root were isolated, and 7 antagonistic bacteria were screened by co-culture with R. solanacearum. The antagonistic endobacteria were identified as Enterobacter arburiae and Pseudomonas indica, respectively. Antagonistic endobacteria exhibit a strong antagonistic activity against R. solanacearum, with their metabolites have an inhibitory rate of over 90% against R. solanacearum. In addition, antagonistic endobacteria also exhibit a strong iron competitive advantage, with the ferritin content in their metabolites significantly higher than that in R. solanacearum metabolites. Experiment showed that the antagonistic endobacteria could successfully colonize in ginger root, significantly reduced the density of R. solanacearum in ginger rhizosphere, and control the incidence of bacterial wilt from 90.10% to 21.43%, showing a good biological control effect. This study provided valuable microbial resources for biological control of ginger blast in southern red soil area in China. Reference | Related Articles | Metrics | Comments（0） Select Development of Wettable Powder of Bacillus velezensis SH-1471 and Its Control Effect on Tomato Fusarium Wilt SHEN Yunxin, LI Minggang, SHI Zhufeng, ZHAO Jiangyuan, WANG Nan, LI Zhefen, YANG Mingying, CHEN Qibin, YANG Peiwen Chinese Journal of Biological Control    2023, 39 (4): 904-914.   DOI: 10.16409/j.cnki.2095-039x.2023.02.040 Abstract （252）      PDF（pc） （461KB）（715）       Knowledge map    Save Bacillus velezensis has great potential in the development of microbial pesticides. The research and development of its wettable powder and the optimization of its production process are one of the measures to promote its industrialization and commercialization. In addition, it is of great significance to promote the green development of agriculture and realize green prevention and control. B.velezensis SH-1471 was used as the research object. Single factor test was used to select the carrier and additive that had the least effect on its activity and its compound proportion. The control effect of B.Velesiensis SH-1471 WP on tomato Fusarium wilt was determined in a pot experiment. The formulation and ratio of B.velezensis SH-1471 WP were established as follows:kaolin as carrier parent powder 87%, sodium lignosulfonate 2%, NNO 8%, xanthan gum 2%, ascorbic acid (VC) 1%; The viable count of the preparation was 2.5×1010 CFU/g, fineness 97%, wetting time 12 s, suspension rate 88.6%, drying reduction 1%, pH 7.1, and impurity rate 0. All the indicators were in line with the national standards. The continuous storage experiments showed that the survival rate of spores reached 87.65% at 4℃ for 180 d and 82.45% at 25℃. Laboratory experiments showed that it had a good control effect and could significantly reduce the incidence of tomato Fusarium wilt, with a control effect of 93.7%. B.Velesiensis SH-1471 WP has the characteristics of good stability and high control efficiency, and has a good application prospect in the biological control of crop diseases. Reference | Related Articles | Metrics | Comments（0） Select Antifungal Mechanism Analysis and Verification of Bacillus velezensis Ba-0321 Based on Whole Genome Sequencing LI Xiaojie, QIU Rui, LIU Chang, YAO Chenxiao, BAI Jingke, CHEN Yuguo, LI Shujun Chinese Journal of Biological Control    2023, 39 (4): 885-894.   DOI: 10.16409/j.cnki.2095-039x.2023.02.035 Abstract （246）      PDF（pc） （2615KB）（3418）       Knowledge map    Save Bacillus velezensis Ba-0321, isolated from the rhizosphere soil of healthy tobacco plants, is a plant growth promoting rhizobacteria with strong antifungal activity. It has a good potential in biocontrol application. In the study, the inhibitory effects of strain Ba-0321 sterile filtrate on Fusarium oxysporum and Phytophthora nicotianae were determined by methods of co-culture. The whole genome of strain Ba-0321 was sequenced using the second-generation Illumina and the third-generation Nanopore platform, then analyzed for genome assembly, gene functional annotation, prediction of secondary metabolite synthetic gene clusters. The results showed that the sterile filtrate of strain Ba-0321 has inhibitory effects on both F. oxysporum and P. nicotianae. The culture medium containing 10% sterile filtrate has a higher inhibitory rate on the mycelial growth of the two pathogens, reaching 57.38% and 34.30%, respectively. The results of whole genome sequencing showed that the genome size of strain Ba-0321 was 4099109 bp, including 3897 coding genes, and the sequencing data are available in the GenBank database (accession No. CP101904). There were a large number of genes encoding enzymes, terpene, polyketone metabolic pathways, and genes involved in defense mechanisms have been annotated in the GO, COG and KEGG databases. Using anti-SMASH software, 13 secondary metabolite synthesis gene clusters were predicted encoding antibacterial substances such as Surfactin、Fengycin、difficidin、Bacillaene、Bacillibactin、Macrolactin H、Bacilysin. The existence of biosynthetic gene cluster for antibacterial substances and defence machainsm related genes in Ba-0321 strain genome were verified by PCR amplification and sequencing. The enzyme activity test results showed that the strain has resistant enzyme activities such as protease, chitinase, and cellulase.. This study provides a basis for analyzing the antimicrobial mechanism of strain Ba-0321 and mining antifungal related gene resources at the genomic level, which is of a great significance for research and application of the Ba-0321 strain in future. Reference | Related Articles | Metrics | Comments（0） Select Screening, Identification, Biological Characteristics of Yeast Strains and Their Antifungal Activities against Botrytis cinerea LAI Chengchun, LAI Gongti, CHEN Bingxing, Pan Ruo, Que Qiuxia, CHE Jianmei Chinese Journal of Biological Control    2023, 39 (3): 594-606.   DOI: 10.16409/j.cnki.2095-039x.2023.02.025 Abstract （243）      PDF（pc） （4325KB）（2140）       Knowledge map    Save Two antagonistic strains G17515 and G31887 were screened out, which showed good biocontrol activities against Botrytis cinerea. The two yeast strains were identified as Starmerella bacillaris and Meyerozyma caribbica by morphological observation and phylogenetic tree analysis. The cultural conditions of G17515 and G31887 were as follows: 20 ℃—30 ℃ (optimum 30 ℃), pH 5—7 (optimum pH 6.0) and 0.1%—5% NaCl (optimum 0.1%). The results of in vitro culture showed that two antagonistic strains G17515 and G31887 could significantly inhibit the mycelial growth of B. cinerea, which inhibit rates were 80.86% and 69.05%, respectively. And the two strains also caused abnormalities in mycelium and spore morphology of B. cinerea. Furthermore, G17515 and G31887 could inhibit the expansion of B. cinerea on grape leaves and fruits. Their inhibitory efficiency on grape leaves reached 64.21% and 57.22%, respectively. And their biocontrol efficiency on grape fruits were 48.56% and 77.13%, respectively. In conclusion, S. bacillaris G17515 and M. caribbica G31887 showed inhibitory effects on B. cinerea, which is expected to be a strain resource for controlling gray mold disease on grapes. Reference | Related Articles | Metrics | Comments（0）