防风根腐病拮抗真菌的筛选鉴定及生防作用研究

doi:10.16409/j.cnki.2095-039x.2022.06.001

中国生物防治学报 ›› 2022, Vol. 38 ›› Issue (5): 1288-1295.DOI: 10.16409/j.cnki.2095-039x.2022.06.001

防风根腐病拮抗真菌的筛选鉴定及生防作用研究

韩忠明¹, 孙卓¹, 王妍¹, 张福军², 马峰敏³, 罗秋菊¹, 杨利民¹

1. 吉林农业大学中药材学院/省部共建生态恢复和生态系统管理国家重点实验室培育基地, 长春 130118;
2. 集安市太王镇林业工作站, 集安 134200;
3. 吉林查干湖国家级自然保护区管理局, 松原 131111

收稿日期:2021-12-27 发布日期:2022-10-20
通讯作者: 杨利民,教授,博士,E-mail:ylmh777@126.com
作者简介:韩忠明,教授,博士,E-mail:hanzm2008@126.com
基金资助:
国家重点研发计划(2019YFC1710700,2019YFC1710702);吉林省科技发展计划项目(20200404010YY,20210204011YY);中央财政林业科技推广示范项目(JLT2020-22,JLT2020-37,JLT2021-03,JLT2021-21);财政部和农业农村部国家现代农业产业技术体系资助(CARS-21)

Screening,Identification and Biological Control of Antagonistic Fungus against Root Rot of Saposhnikovia divaricata

HAN Zhongming¹, SUN Zhuo¹, WANG Yan¹, ZHANG Fujun², MA Fengmin³, LUO Qiuju¹, YANG Limin¹

1. College of Chinese Medicinal Materials, Jilin Agricultural University, Key Laboratory for Ecological Restoration and Ecosystem Management of Jilin Province, Changchun 130118, China;
2. Forestry Working Station of Taiwang Town in Ji'an, Ji'an 134200, China;
3. Chagan Lake National Nature Reserve Administration in Jilin, Songyuan 131111, China

Received:2021-12-27 Published:2022-10-20

摘要/Abstract

摘要： 从防风根际土壤中筛选得到1株对根腐病具有良好防治效果的拮抗菌株MR-47，基于形态学、ITS序列分析方法对菌株MR-47进行鉴定，确定该菌株为桃色顶孢霉Acremonium persicinum（GenBank No. OK287149.1）。通过对峙培养、显微镜观察，探究了菌株MR-47对木贼镰刀菌Fusarium equiseti的抑菌机理；基于盆栽试验，明确菌株MR-47的定殖能力及防病促生效果。结果表明，菌株MR-47对木贼镰刀菌抑菌率达69.26%，病原菌菌丝出现缢缩、膨大、扭曲、畸形等现象；对尖孢镰刀菌、腐皮镰刀菌等多种病原真菌表现出了良好的广谱抑菌活性；在土壤中定殖35 d内，最大定殖量为8.38×10⁶ CFU/g，最低为5.57×10⁶ CFU/g；MR-47对防风根腐病防效达75.05%。因此，桃色顶孢霉MR-47在防风根腐病的病害管理方面具有较好的开发价值及应用潜力。

关键词: 防风, 桃色顶孢霉, 木贼镰刀菌, 生物防治, 促生作用

Abstract: The strain MR-47 isolated from rhizospheric soil of Saposhnikovia divaricata plants was screened and selected as a potential stock for biocontrol due to the significantly antibiotic activity against root rot disease caused by Fusarium equiseti.Based on the analysis of morphological traits and rDNA internal transcribed spacers (ITS),strain MR-47 was identified as Acremonium persicinum(GenBank No.OK287149.1).The antifungal mechanism of strain MR-47 against F.equiseti were determined by using dual culture assays and microscopic observation.To assess the antifungal efficacy of MR-47 against root rot disease of S.divaricata as well as its colonization ability and growth promotion ability,pot experiments were performed under natural outdoor conditions.The results indicated that MR-47 had the strongest inhibition activity against F.equiseti,with the inhibition rate up to 69.26%.The fermentation liquor of MR-47 could significantly induce constriction,enlargement,distortion and deformity in mycelia morphology of F.equiseti.Strain MR-47 had the broad-spectrum antibiotic activity against pathogens,such as F.oxysporum and F.solani.The population of MR-47 in soil in which S.divaricata was grown,were from 5.57×10⁶ CFU/g to 8.38×10⁶ CFU/g.Strain MR-47 spore suspension delayed the occurrence of S.divaricata root rot disease and had control efficacy of 75.05%.A.persicinum MR-47 had the potential to be developed into biocontrol agent for comtrol of S.divaricata root rot disease.

Key words: Saposhnikovia divaricata, Acremonium persicinum, Fusarium equiseti, biological control, growth promotion

中图分类号:

S476

韩忠明, 孙卓, 王妍, 张福军, 马峰敏, 罗秋菊, 杨利民. 防风根腐病拮抗真菌的筛选鉴定及生防作用研究[J]. 中国生物防治学报, 2022, 38(5): 1288-1295.

HAN Zhongming, SUN Zhuo, WANG Yan, ZHANG Fujun, MA Fengmin, LUO Qiuju, YANG Limin. Screening,Identification and Biological Control of Antagonistic Fungus against Root Rot of Saposhnikovia divaricata[J]. Chinese Journal of Biological Control, 2022, 38(5): 1288-1295.

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防风根腐病拮抗真菌的筛选鉴定及生防作用研究

Screening,Identification and Biological Control of Antagonistic Fungus against Root Rot of Saposhnikovia divaricata

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