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Chinese Journal of Biological Control ›› 2021, Vol. 37 ›› Issue (2): 340-348.DOI: 10.16409/j.cnki.2095-039x.2021.03.012

• SCIENTIFIC NOTES • Previous Articles    

Cloning of Sodium Channel Genes of Drosophila simulans and Its Bioinformatics Analysis

DENG Denghui1, DUAN Wenbo1, WANG Hao1, LI Fen2, WU Shaoying1,2   

  1. 1. College of Plant Protection, Henan Agricultural University, Zhengzhou 450000, China;
    2. College of Plant Protection, Hainan University, Haikou 570228, China
  • Received:2020-04-07 Published:2021-04-14

Abstract: Voltage-gated sodium channel gene of Drosophila simulans was cloned using RT-PCR and typical characteristics and phylogenetic relationship of the gene were clarified. The open reading frame of the gene (GenBank number of MT113357) was 6270 bp in length and encoded 2090 amino acids. Bioinformatics analysis showed a 96.86% similarity of the sodium channel genes of D. simulans and Drosophila melanogaster, a 88.75% similarity of the sodium channel genes of D. simulans and Musca domestica, and a very high degree of similarity in the sodium channel genes of D. simulans and Diptera insects. The cloned sequence was of the basic characteristics of insect sodium channels, with a DEKA module and a hydrophobic inactivation gate MFM. Three alternative splicing exons i, a, and b and 17 RNA editings were obtained. The present cloning and analysis of the sodium channel gene of D. simulans serve to provide a foundation for resistance mechanism study and new ideas for the development of new agricultural pesticides.

Key words: Drosophila simulans, insect sodium channel, cloning, alternative splicing, bioinformatics analysis

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