Identification of Radiation-resistant Bacillus velezensis JK23 and Its Biocontrol Activity

doi:10.16409/j.cnki.2095-039x.2021.06.011

Abstract

Abstract: Strain JK23 is a radiation-resistant Bacillus sp. stored in our radiation-resistant microbial resource bank. Through systematic morphological, physiological and biochemical experiments and molecular identification, strain JK23 was confirmed as Bacillus velezensis. Plate confrontation experiment showed that strain JK23 had significant inhibitory effect on a variety of plant pathogens, and the inhibitory rates were as follows:Alternaria alternaria (39.68%), Fusarium oxysporum SCC-F5 (37.5%), Fusarium moniliforme SCC-F11 (50.0%), Fusarium oxysporum (53.85%), Alternaria solani (73.3%), Didymella bryoniae (100%) and Cytcospora sp. (100%). Meanwhile, using microscopic observation, strain JK23 demonstrated inhibiting effect on the growth of mycelia of pathogenic fungi, causing mycelia malformation, enlargement and pynosis. Genetic analysis and MALDI-TOF MS further discovered that strain JK23 could synthesize a large number of lipopeptide antibiotics, which had strong hemolysis and oil discharge characteristics. In view of the lipopeptide activity and inhibitory effect of B. velesiensis JK23, it has broad prospect for development and application in future.

Key words: Bacillus velezensis, lipopeptide, antifungal activity

CLC Number:

S476

ZHANG Lijuan, HUANG Wei, WANG Ning, SONG Bo, ZHU Jing, ABDUKEYIM Rayhangul, ABBAS Bujannat, WANG Wei. Identification of Radiation-resistant Bacillus velezensis JK23 and Its Biocontrol Activity[J]. Chinese Journal of Biological Control, 2021, 37(5): 997-1006.

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