Evaluation of Biocontrol Potential of the Antagonistic Actinomycetes against Phytophthora parasitica var. nicotianae

doi:10.16409/j.cnki.2095-039x.2023.02.024

Abstract

Abstract: Tobacco black shank caused by P. parasitica var. nicotianae was one of the important soil-borne diseases in continuous cropping tobacco fields. When the disease occured seriously, it often causes huge economic losses to tobacco farmers. 179 strains of actinomycetes were isolated from healthy tobacco rhizosphere soil in tobacco black shank serious disease area in Zunyi City, Guizhou Province. Fifteen strains with obvious inhibitory effects on the mycelial growth of P. parasitica var. nicotianae were screened by dual culture method. The inhibitory effect of the metabolic fluid of these 15 strains on the mycelial growth of P. parasitica var. nicotianae was determined. The results showed that the metabolic fluid of the strain H-3 had the highest inhibitory rate on the mycelial growth, and the inhibition rate was 85.68%. The inhibitory effect of the metabolic fluid strain H-3 on zoospore germination of P. parasitica var. nicotianae was studied. The results showed that the inhibition rates of zoospore germination for 6 h, 12 h and 24 h were 72.75%, 65.20% and 63.45%, respectively. The strain H-3 was identified as Streptomyces sp. by morphological characteristics and 16S rDNA gene sequence analysis. Pot experiment and field experiment showed that H-3 had a certain control effect on tobacco black shank. The potted control effect was 55.99%. When H-3 solid fermentation was added at a dosage of 22.5 kg/hm², the field control effect was the best, which could reached 70.42%. The copy numbers of P. parasitica var. nicotianae in tobacco rhizosphere soil was detected by the method of fluorescence quantitative PCR after application of the strain H-3 solid fermentation. The fluorescence quantitative PCR results showed that the copy numbers of P. parasitica var. nicotianae in tobacco rhizosphere soil decreased with the increasing of H-3 dosage, which indicated that the strain H-3 could effectively reduce the number of P. parasitica var. nicotianae in tobacco rhizosphere soil.

Key words: Phytophthora parasitica var. nicotiana, actinomycetes, inhibition rate of mycelial growth, antagonistic effect, field efficacy, fluorescence quantitative PCR

CLC Number:

S476

ZHANG Ge, PENG Yulong, GOU Jianyu, HE Kai, LI Zhanghai, WANG Zhangxun, QI Yongxia, teshuzifu. Evaluation of Biocontrol Potential of the Antagonistic Actinomycetes against Phytophthora parasitica var. nicotianae[J]. Chinese Journal of Biological Control, 2023, 39(3): 667-675.

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