Screening, Identification, Biological Characteristics of Yeast Strains and Their Antifungal Activities against Botrytis cinerea

doi:10.16409/j.cnki.2095-039x.2023.02.025

Abstract

Abstract: Two antagonistic strains G17515 and G31887 were screened out, which showed good biocontrol activities against Botrytis cinerea. The two yeast strains were identified as Starmerella bacillaris and Meyerozyma caribbica by morphological observation and phylogenetic tree analysis. The cultural conditions of G17515 and G31887 were as follows: 20 ℃—30 ℃ (optimum 30 ℃), pH 5—7 (optimum pH 6.0) and 0.1%—5% NaCl (optimum 0.1%). The results of in vitro culture showed that two antagonistic strains G17515 and G31887 could significantly inhibit the mycelial growth of B. cinerea, which inhibit rates were 80.86% and 69.05%, respectively. And the two strains also caused abnormalities in mycelium and spore morphology of B. cinerea. Furthermore, G17515 and G31887 could inhibit the expansion of B. cinerea on grape leaves and fruits. Their inhibitory efficiency on grape leaves reached 64.21% and 57.22%, respectively. And their biocontrol efficiency on grape fruits were 48.56% and 77.13%, respectively. In conclusion, S. bacillaris G17515 and M. caribbica G31887 showed inhibitory effects on B. cinerea, which is expected to be a strain resource for controlling gray mold disease on grapes.

Key words: Botrytis cinerea, yeast, biological characteristic, mycelium, antagonism

CLC Number:

S476

LAI Chengchun, LAI Gongti, CHEN Bingxing, Pan Ruo, Que Qiuxia, CHE Jianmei. Screening, Identification, Biological Characteristics of Yeast Strains and Their Antifungal Activities against Botrytis cinerea[J]. Chinese Journal of Biological Control, 2023, 39(3): 594-606.

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