Screening and Biocontrol Characteristics of Endophytic Strains against Ginger Bacterial Wilt

doi:10.16409/j.cnki.2095-039x.2023.02.030

Abstract

Abstract: Ginger bacterial wilt is a soil borne bacterial disease caused by Ralstonia solanacearum, which seriously restricts the development of ginger industry. In this study, endobacteria from ginger root were isolated, and 7 antagonistic bacteria were screened by co-culture with R. solanacearum. The antagonistic endobacteria were identified as Enterobacter arburiae and Pseudomonas indica, respectively. Antagonistic endobacteria exhibit a strong antagonistic activity against R. solanacearum, with their metabolites have an inhibitory rate of over 90% against R. solanacearum. In addition, antagonistic endobacteria also exhibit a strong iron competitive advantage, with the ferritin content in their metabolites significantly higher than that in R. solanacearum metabolites. Experiment showed that the antagonistic endobacteria could successfully colonize in ginger root, significantly reduced the density of R. solanacearum in ginger rhizosphere, and control the incidence of bacterial wilt from 90.10% to 21.43%, showing a good biological control effect. This study provided valuable microbial resources for biological control of ginger blast in southern red soil area in China.

Key words: ginger bacterial wilt, antagonistic endobacteria, Enterobacter asburiae, Pseudomonas indica, biological control

CLC Number:

S476

DANG Keke, PENG Junwei, SUN Yang, XIE Ziyu, DONG Yuanhua, LI Jiangang. Screening and Biocontrol Characteristics of Endophytic Strains against Ginger Bacterial Wilt[J]. Chinese Journal of Biological Control, 2023, 39(3): 585-593.

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