Biocontrol Efficiency and Mechanism of Bacillus tequilensis KXF 6501 against Grey Mold in Postharvest Kiwifruit

doi:10.16409/j.cnki.2095-039x.2023.02.042

Abstract

Abstract: Kiwifruit gray mold disease caused by Botrytis cinerea is one of the main postharvest diseases. In order to better control postharvest gray mold in kiwifruit, this study used Bacillus tequilensis KXF 6501 isolated from healthy strawberry fruit surfaces as the test strain, its antagonistic effect against gray mold on plate and kiwifruit fruits was investigated, then its effect on kiwifruit fruits resistance substances, resistance defense enzyme activities and fruit quality were studied. The results showed that the 24 h- and 96 h-fermentation broths of KXF 6501 inhibited gray mold by 75.96% and 73.72%, respectively, at the 5th day on plate, and the fruit incidence of gray mold disease on kiwifruit was 65.00% and 70.00%, respectively, at the 4th day of storage, which significantly lower than the control fruits. The lesion diameters on the kiwifruit at 11d were 2.78 and 4.25 cm, respectively, which were smaller than those of the control fruits. The 24 h fermentation broth showed a better control effect. Compared with the control, 24 h-fermentation broth of KXF 6501 induced a faster increase of resistance substance content and enzyme activity in kiwifruit. The content of total phenols, flavonoids, and anthocyanins reached their peak at 7 d, which were 1.96, 4.07, and 5.29 times higher than those of the control group, respectively. The peak PAL, GLU and CHI enzyme activities were 95.04, 240.19 and 8.41 U/g, respectively, which were 2.40, 2.03 and 1.15 times higher than those of the control group, and the PPO enzyme activity was significantly higher than that of the control group at 28 d, which was 116.58 U/g, 3.35 times higher than that of the control group. In addition, 24 h-fermentation broth of KXF 6501 treatment reduced fruit soluble solids content and weight loss, maintained the nutritional quality of kiwifruit fruits. The study showed that Bacillus tequilensis KXF 6501 had a good inhibitory effect on the kiwifruit gray mold pathogen, reduced the incidence of the disease, and could increase the content and activities of disease resistance-related substances and enzymes in kiwifruit fruits, then induced the systemic resistance in kiwifruit fruits.

Key words: Bacillus tequilensis, kiwifruit, postharvest disease, biological control

CLC Number:

S476

LI Raoyong, DONG Kuikui, YANG Kuo, SHI Xiumei, WANG Meilin, DENG Jia, WANG Fang. Biocontrol Efficiency and Mechanism of Bacillus tequilensis KXF 6501 against Grey Mold in Postharvest Kiwifruit[J]. Chinese Journal of Biological Control, 2023, 39(4): 933-941.

References

[1] 张维, 黄余年, 张群, 等. 猕猴桃采后贮藏保鲜技术研究进展[J]. 保鲜与加工, 2021, 21(5):139-145.
[2] 刘霞, 袁海英, 任云, 等. 灰霉菌侵染对采后猕猴桃果实生理生化指标的影响[J]. 经济林研究, 2019, 37(2):41-48.
[3] Li X, Wang T, Zhou B, et al. Chemical composition and antioxidant and anti-inflammatory potential of peels and flesh from 10 different pear varieties (Pyrus spp.)[J]. Food chemistry, 2014, 152:531-538.
[4] 朱克永, 隋明. 猕猴桃加工工艺及开发利用趋势[J]. 食品研究与开发, 2018, 39(22):220-224.
[5] 高磊, 罗轩, 张蕾, 等. 猕猴桃采后真菌腐烂病害发生与防治技术研究进展[J]. 中国果树, 2018(3):72-76.
[6] Michailides T J, Elmer P A G. Botrytis gray mold of kiwifruit caused by Botrytis cinerea in the United States and New Zealand[J]. Plant Disease, 2000, 84(3):208-223.
[7] Di Francesco A, Mari M, Ugolini L, et al. Effect of Aureobasidium pullulans strains against Botrytis cinerea on kiwifruit during storage and on fruit nutritional composition[J]. Food Microbiology, 2018, 72:67-72.
[8] 邹曼, 陈雨诗, 许春艳, 等. 生防芽胞杆菌对果蔬采后病害防治的研究进展[J]. 食品工业科技, 2023, 44(4):488-495.
[9] 奉夏平, 宁焕焱, 黄飞, 等. 高效液相色谱-串联质谱法同时测定5种鲜水果保鲜剂的残留量[J]. 分析测试学报, 2013, 32(12):1482-1486.
[10] Alvarez A, Gelezoglo R, Garmendia G, et al. Role of Antarctic yeast in biocontrol of Penicillium expansum and patulin reduction of apples[J]. Environmental Sustainability, 2019, 2:277-283.
[11] Chen K, Tian Z, He H, et al. Bacillus species as potential biocontrol agents against citrus diseases[J]. Biological control, 2020, 151:104419.
[12] Singh A K, Sharma P. Disinfectant-like activity of lipopeptide biosurfactant produced by Bacillus tequilensis strain SDS21[J]. Colloids and Surfaces B:Biointerfaces, 2020, 185:110514.
[13] 何明川, 王志江, 谢永辉, 等. 烟草黑胫病拮抗菌的筛选、鉴定及发酵条件优化[J]. 中国生物防治学报, 2022, 38(2):428-439.
[14] 郗良卿, 张士凯, 陈雨诗, 等. "美早"甜樱桃褐腐病病原菌的分离及内生拮抗细菌的筛选鉴定[J]. 食品与发酵工业, 2020, 46(8):85-91.
[15] 申建芳, 李子桀, 蒙春燕, 等. 马铃薯黄萎病生防细菌的筛选与鉴定[J]. 北方农业学报, 2018, 46(1):81-84.
[16] 谢洁, 任慧爽, 唐翠明, 等. 一株桑树内生细菌的鉴定和对桑椹核地杖菌的拮抗作用[J]. 蚕业科学, 2015, 41(5):815-824.
[17] Shafi J, Tian H, Ji M. Bacillus species as versatile weapons for plant pathogens:a review[J]. Biotechnology & Biotechnological Equipment, 2017, 31(3):446-459.
[18] 唐文, 梁艳琼, 许沛冬, 等. 枯草芽胞杆菌Czk1诱导橡胶树抗病性相关防御酶系研究[J]. 南方农业学报, 2016, 47(4):576-582.
[19] Bartwal A, Mall R, Lohani P, et al. Role of secondary metabolites and brassinosteroids in plant defense against environmental stresses[J]. Journal of Plant Growth Regulation, 2013, 32:216-232.
[20] Abdelkhalek A, Al-Askar A A, Behiry S I. Bacillus licheniformis strain POT1 mediated polyphenol biosynthetic pathways genes activation and systemic resistance in potato plants against Alfalfa mosaic virus[J]. Scientific Reports, 2020, 10(1):1-16.
[21] Zhou H, Zhu H, Ren Z, et al. Efficacy of Bacillus tequilensis strain JN-369 to biocontrol of rice blast and enhance rice growth[J]. Biological Control, 2021, 160:104652.
[22] Karthika S, Remya M, Varghese S, et al. Bacillus tequilensis PKDN31 and Bacillus licheniformis PKDL10-As double headed swords to combat Fusarium oxysporum f. sp. lycopersici induced tomato wilt[J]. Microbial Pathogenesis, 2022, 172:105784.
[23] 马电通, 吕鹤飞, 常雪苗, 等. 羧甲基纤维素诱导提高罗伦隐球酵母对采后砂糖橘抗青霉、绿霉病的生防效果[J]. 食品科学, 2022, 43(21):241-247.
[24] 曹建康, 姜微波, 赵玉梅. 果蔬采后生理生化实验指导[M]. 北京:中国轻工业出版社, 2007.
[25] Lastochkina O, Seifikalhor M, Aliniaeifard S, et al. Bacillus spp.:efficient biotic strategy to control postharvest diseases of fruits and vegetables[J]. Plants, 2019, 8(4):97.
[26] 赵焕兰, 曹嘉灿, 刘奎, 等. 贝莱斯芽胞杆菌发酵液对樱桃番茄贮藏品质的影响[J]. 食品研究与开发, 2022, 43(20):12-18.
[27] Jinal N H, Amaresan N. Evaluation of biocontrol Bacillus species on plant growth promotion and systemic-induced resistant potential against bacterial and fungal wilt-causing pathogens[J]. Archives of microbiology, 2020, 202(7):1785-1794.
[28] Zhang X, Kong W, Wu X, et al. Bacillus velezensis JK-XZ8 prevents and controls crown gall disease on Prunus subhirtella by colonizing and inducing resistance[J]. Journal of Forestry Research, 2022, 33(3):1019-1031.
[29] 田登娟, 孔珊珊, 马电通, 等. 羧甲基壳聚糖诱导培养罗伦隐球酵母处理调控采后葡萄柚果实苯丙烷代谢及其抗青霉病作用[J/OL]. 食品科学, 2023:1-9.
[30] 陈刘军, 俞仪阳, 王超, 等. 蜡质芽胞杆菌AR156防治水稻纹枯病机理初探[J]. 中国生物防治学报, 2014, 30(1):107-112.
[31] Lu Y, Ma D, He X, et al. Bacillus subtilis KLBC BS6 induces resistance and defence-related response against Botrytis cinerea in blueberry fruit[J]. Physiological and Molecular Plant Pathology, 2021, 114:101599.
[32] 蔡孟轩, 邓丽莉, 姚世响, 等. CaCl₂增强橄榄假丝酵母对苹果果实采后青霉病的生防效力[J]. 食品科学, 2019, 40(11):220-226.
[33] Wu Y, Lin H, Lin Y, et al. Effects of biocontrol bacteria Bacillus amyloliquefaciens LY-1 culture broth on quality attributes and storability of harvested litchi fruit[J]. Postharvest Biology and Technology, 2017, 132:81-87.
[34] Romanazzi G, Feliziani E, Baños S B, et al. Shelf life extension of fresh fruit and vegetables by chitosan treatment[J]. Critical Reviews in Food Science and Nutrition, 2017, 57(3):579-601.
[35] 柴庆凯, 张斌, 常若葵, 等. 解淀粉芽胞杆菌LJ02对黄瓜抗灰霉病菌的生防效果及其诱导抗性机理的初步研究[J]. 植物病理学报, 2019, 49(6):828-835.