Screening and Identification of Biocontrol Potential Myxobacteria Strains against Fire Blight and Pear Canker Diseases and Evaluation of Indoor Control Efficacy

doi:10.16409/j.cnki.2095-039x.2023.02.059

Abstract

Abstract: Fire blight causted by Erwinia amylovora and pear canker causted b Valsa pyri are two major diseases affecting the production of Pyrus sinkiangensis. This study aimed to develop biological control agent for combating fire blight and pear canker diseases by utilizing myxobacteria strains isolated from soil samples collected from different prefectures in Xinjiang. The predation efficiency of myxobacteria strains on E. amylovora was compared by using bacterial lawn co-culture, while the inhibitory effect of myxobacteria strains on hyphal growth and conidial germination of V. pyri was assessed by the pair-culuturing method. Myxobacteria strains with significant predation efficacy and antagonistic activities against E. amylovora and V. pyri were screened, and the control effects of the strains on these two diseases were further evaluted by detached leaves, branches and greenhouse potted experiments. The myxobacteria strains with biocontrol potential were identified based on morphological characteristics, 16S rRNA and lep A gene sequence homology analysis. The results showed that the six selected myxobacteria strains exhibited substantial predation effects on E. amylovora, demonstrated by significant reduction of the bacterial numbers from 10⁹ to 10²～10⁵ cfu/mL. Additionally, these strains demonstrated strong antagonistic activity against V. pyri, with inhibition rates of mycelial growth and conidial germination over 70% and 87%, respectively. Strains NST12 and NST47 showed protective and therapeutic effects of over 75% against fire blight on detached P. sinkiangensis leaves and P. betulifolia seedlings, while strains NST47 and NST49 exhibited protective and therapeutic effects of over 72% and 54% against pear canker on detached twigs, respectively. Notably, strain NST47 displayed remarkable biocontrol potential against both fire blight and pear canker. Based on the morphological and molecular identification results, strains NST49 and NST12 were identjfied as Myxococcus fulvus and NST47 was identjfied as Archangium violaceum.

Key words: fire blight, pear cankeri, biological control, myxobacteria

CLC Number:

S476

BAI Xueying, HAN Jian, SUN Boyuan, SONG Shumin, LUO Ming, SUN Liying. Screening and Identification of Biocontrol Potential Myxobacteria Strains against Fire Blight and Pear Canker Diseases and Evaluation of Indoor Control Efficacy[J]. Chinese Journal of Biological Control, 2023, 39(6): 1384-1397.

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