Functional Study of the Gene Cluster xtlABC Producing Cyclic Lipopeptide in Pseudomonas mosselii PtA1

doi:10.16409/j.cnki.2095-039x.2024.02.023

Abstract

Abstract: Previous studies have demonstrated that Pseudomonas mosseliiPtA1 displays high levels antibacterial activities against various strains of Ralstonia solanacearum. Its genome encompasses a complete xtlABC gene cluster, which is responsible for the production of cyclic lipopeptide. In order to explore the function of xtlABC gene cluster in P. mosseliiPtA1, we employed homologous recombination to create deletion mutants ΔxtlA, ΔxtlBC and ΔxtlABC, verified through Southern blot analysis, and assessed the phenotypic changes in growth rate, swimming motility, biofilm formation and antagonistic activity. The results revealed that compared with the wild-type strain PtA1, mutants ΔxtlA,ΔxtlBC and ΔxtlABCdisplayed a significant reduction in growth rate. The diameters of swimming rings were diminished by 12.6 mm, 12.5 mm and 13.5 mm, respectively, the amount of biofilm formation decreased by 30.41%, 29.16% and 30.70%, respectively, and the diameters of the antibacterial zone against R. solanacearum were also notably reduced. Pot experiments demonstrated that the control efficacy of treatment groups PtA1+GMI1000, ΔxtlA+GMI1000,ΔxtlBC+GMI1000 and ΔxtlABC+GMI1000 on tomato bacterial wilt were 44.93%, 9.49%, 10.46% and 7.66% 4 days post-inoculation with R. solanacearum GMI1000, respectively. The control efficacies were 42.43%, 19.56%, 31.40% and 24.79% 10 days post-inoculation, respectively. The treatment group PtA1+GMI1000 had the best control effect on tomato bacterial wilt, significantly different compared to the xtlABC mutant treatment groups. These findings underscore the impact of the xtlABC gene cluster on the growth and biocontrol-related characteristics of P. mosselii PtA1, and its secondary metabolite played an important role in inhibiting R. solanacearum.

Key words: Pseudomonas mosselii, cyclic lipopeptide, <xtlABC, Ralstonia solanacearum, antibacterial activity

CLC Number:

S476

ZHENG Hanwei, XIE Wensong, NIU Shilin, ZHANG Xu, ZHUO Tao, QIU Sixin, FAN Xiaojing. Functional Study of the Gene Cluster xtlABC Producing Cyclic Lipopeptide in Pseudomonas mosselii PtA1[J]. Chinese Journal of Biological Control, 2024, 40(3): 620-629.

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