Metabolite of Symbiotic Bacteria from Steinernema feltiae SRP 18-91 and Their Antibacterial Activity

doi:10.16409/j.cnki.2095-039x.2024.02.032

Abstract

Abstract: This study aimed to determine the inhibitory effect of the fermentation broth of symbiotic bacteria from Steinernema feltiae SRP 18-91 on 5 plantpathogenic fungi by mycelial growth. The results suggested that the Colletotrichum fragariae Brooks and Phytophthora sojae Kaufmann & Gedemann had high sensitivity to broth, with inhibitory rates of 96.8% and 90.8%, respectively. Maximum effectiveness of fermentation broth against Heterodera glycines Ichinohe was 61.47% in soybean pot culture experiment. The symbiotic bacteria of S. feltiae SRP 18-91 was identified to be a Xenrohabdus bovineii SRP 18-91 by 16S rRNA sequence analysis. Further study showed that the major compounds of the liquid extract from symbiotic bacteria were triterpenoid, valerate metabolites, pentamycin, hydroxyquinoline, and amino acid compound by LC/MS. The abundance of 8-hydroxyquinoline was the highest in all the components.

Key words: entomopathogenic nematode, symbiotic bacterium, metabolic product, antifungal activity

CLC Number:

S476

ZHANG Jinhua, TIAN Chengli, SU Lanqi, SUN Hui, DING Yan, LI Maohai, LI Li. Metabolite of Symbiotic Bacteria from Steinernema feltiae SRP 18-91 and Their Antibacterial Activity[J]. Chinese Journal of Biological Control, 2024, 40(5): 1128-1134.

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