Virulence of Entomopathogenic Nematodes to Holotrichia parallela Larvae

doi:10.16409/j.cnki.2095-039x.2022.03.025

Abstract

Abstract: The pathogenicity of seven entomopathogenic nematodes (EPNs) against the late 1^st instar larvae of Holotrichia parallela was tested in laboratory to screen the effective strains of entomopathogenic nematodes for the H. parallela control, and the most suitable environmental conditions of strains were identified. Results showed that pathogenicity of seven EPNS strains were different under laboratory conditions, the Heterorhabditis bacteriophora (D26-6) had the strongest pathogenicity to the larvae of H. parallela. In addition, temperature, soil moisture and dosage of EPNs all take effects on the pathogenicity. The optimum temperature and soil moisture for H. bacteriophora (D26-6) infection were 25 ℃ and 18% respectively, when the ratio of H. parallela larvae to D26-6 nematodes was 1:100, the mortality of H. parallela larvae can reach 100% after 11 days.

Key words: entomopathogenic nematodes, Heterorhabditis bacteriophora, Holotrichia parallela, pathogenicity

CLC Number:

S476

ZHANG Cailing, WANG Zhimin, WANG Siyu, Ibrahim Osman, LI Kebin, ZHANG Shuai, CAO Yazhong, WANG Senshan, YIN Jiao. Virulence of Entomopathogenic Nematodes to Holotrichia parallela Larvae[J]. Chinese Journal of Biological Control, 2024, 40(5): 1009-1014.

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