Entomogenous Fungus Diversity of Four Habitats in Jiulong Mountain Nature Reserve

doi:10.16409/j.cnki.2095-039x.2024.02.057

Abstract

Abstract: In this study, soil samples from four different habitats (tea garden, sweet potato field, plantation and primary forest) in Jiulongshan National Nature Reserve of ZheJiang Province were used as research materials. Effective data were obtained by illumina NovaSeq sequencing platform for analysis of the fungal diversity and community structure of entomogenous fungi in 20 soil samples under four different habitats, and the correlation between entomogenous fungus and soil environmental factors, The main purpose was to clarify the diversity and distribution characteristics of soil entomogenous fungus in different habitats and provide scientific basis for the development and utilization of entomogenous fungus and species diversity in Jiulongshan National Nature Reserve, Zhejiang Province. The results showed that the relative abundance of entomogenous fungus in forests (primary forest, plantation) was higher than that in agricultural land (tea garden, sweet potato field) under four habitats. There was a significant difference in the entomogenous fungus communities in the soils of the four habitats. The dominant genera in the primary forest and plantation were Tolypocladiumi, while those in the tea garden and sweet potato field were Fusarium and Metarhizium respectively. The ratio of carbon to nitrogen and total nitrogen in soils were significant environmental factors affecting the entomogenous fungus in the region. In forest soils, entomogenous fungus floras was positively correlated with soil carbon to nitrogen ratio and total nitrogen level, while in agricultural soils, the contents of total phosphorus and available phosphorus were positively correlated with entomogenous fungus communities. Among the top ten genera in the relative abundances of soil entomogenous fungi in four habitats, Metarhizium, Isaria and Cordyceps were significantly positively correlated with soil carbon to nitrogen ratio and total nitrogen level, while Fusarium was significantly negatively correlated with soil carbon to nitrogen ratio. The results showed that there were rich entomogenous fungi species in Jiulongshan Nature Reserve, habitat type influenced the abundance of entomogenous fungi, and the relative abundance of entomogenous fungi in the soils of forest was higher than that in the four different habitats. The dominant entomogenous fungi mainly concentrated in three genera, such as Metarhizium, Tolypocladium and Fusarium.

Key words: Jiulongshan nature reserve, entomogenous fungus, different habitat, NovaSeq, diversity

CLC Number:

S476

ZHAO Yuxia, CHEN Lihua, JIA Xiaoqi, SHAO Zhaoyang, SHU Jinping, ZHANG Yabo. Entomogenous Fungus Diversity of Four Habitats in Jiulong Mountain Nature Reserve[J]. Chinese Journal of Biological Control, 2024, 40(5): 1135-1148.

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